Abstract: Objective To analyze the gene and protein sequence of a novel gene GP1 with bioinformatics and explore its action in process of the gastric carcinogenesis and development. Methods Based on the human genome resource, Megablast and SAGE were used for the analysis of chromosome location and tissue expression of GP1; BLAST procedure was used to analyze the gene sequence and the similarity gene; ORF finder, ProParam, ProtScale and so on have been performed on GP1, sequence of the GP1 encoding protein, the physical and chemical characteristics, subcellular localization, functional domain and so on are predicted. Results The full length of the GP1 is 1362bp, the longest ORF is 801bp and encoded 267 amino with a low homologous eIF5C domain.GP1 gene is expressed in different tissues. Conclusion Bioinformatic analysis of GP1 showed that GP1 is a gastric cancer associated gene and may be involved in the process of the gastric carcinogenesis and development.