Abstract: Objective This paper aims to generate a cell line overexpressing SOCS1 protein and investigate the protective effect of SOCS-1 on apoptosis of pancreatic β cell. Methods The rat SOCS-1 coding sequence was amplified and subcloned into pEGFP-C1 vector. The recombinant plasmid pEGFP-C1-SOCS1 was transfected into RINm5F cell line (insulin-secreting tumor cell line). We generated a rat RINm5F cell line overexpressing recombinant plasmid pEGFP-C1-SOCS1. Cells were treated by several cytokines, including TNF-α, IL-1β, IFN-r, IL-1β+IFN-r, IL-1β+TNF-α+IFN-r. The cellular apoptosis were measured by RT-PCR and Western blot. Results We have generated a rat RINm5F cell line overexpressing SOCS1 protein successfully. The expression level of the iNOS mRNA was significantly increased in RINm5F cells after treated by IFN-r, IL-1β+IFN-r, IL-1β+TNF-α+IFN-r, but not SOCS1-RINm5F cells. Western blot analysis demonstrated that the expression and activity of caspase-3 was significantly increased in RINm5F cells after treated by all cytokines. However, the caspase-3 expression and activity was not significantly different in SOCS1-RINm5F cells after treated by cytokines. Conlusion Overexpression of SOCS-1 can reduced cytokine-induced pro-apoptotic gene expression in pancreatic β cells.