Abstract: Objective We analyzed the prostate biopsy data obtained with a transperineal ultrasound and thereafter navigated the 11-region prostate biopsy template in a sample population to evaluate prostate cancer spatial distribution characteristics. Methods From May 2004 to December 2007, 215 patients with a median prostate-specific antigen (PSA) level of 21.0 ng/ml (range 1.2 to 100) were diagnosed with prostate cancer through the transperineal ultrasound guided 11-region template prostate biopsy at Peking Union Medical College Hospital. The characteristics of our sample cancer spatial distribution were assessed in relation with different PSA levels. Results The mean positives for the cancer of regions 1-10 and region 11 (the apical region) were 61.2% vs. 66.4% in patients whose PSA > 20ng/ml , and 35.7% vs. 47.6% in patients with PSA ≤ 20ng/ml (P<0.001). The positives for cancer contained within the anterior and posterior areas were 96.5% vs. 90.9% in patients with PSA > 20ng/ml and 75.2% vs. 75.2% in patients with PSA ≤ 20ng/ml. Conclusions Our findings suggest that there is no difference in cancer distribution between anterior and posterior parts of prostate. Prostate cancers in PSA level less than 20ng/ml patients are more frequently found in the apical region, but no different rate is found in these regions in higher PSA level patients.