Table of Content

    05 June 2010, Volume 30 Issue 6
    Effects of different conditions on rat bone marrow mesenchymal stem cells differentiating into cardiomyocytes in vitro
    Yan-mei ZHAO; Guo-qiang ZHONG; Hong-hong KE; Jin-yi LI
    2010, 30(6):  561-565. 
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    Objective After inducing and differentiating by different ways, we investigated the ability that mesenchymal stem cells( MSCs) differentiating into cardiomyocytes(CMs) and studied the differences that MSCs express cardiac cell-specific markers.Methods MSCs and CMs were isolated and cultivated, then MSCs were induced by 5-Aza-2'-deoxycytidine(5-aza), cocultured with CMs and cultured alone after labeled by 4 ,6-dimidino-2-phenylindone( DAPI) at the third passage. On the 1,2,3 and 4 weeks , DAPI-MSCs were observed the cell morphology and identified troponinT (cTnT) and connexin(Cx)43 by immunohistochemistry.Results DAPI-MSCs was't found shrinkage and the expression of cTnT and Cx43 in the control group. In the induction group and the co-culture group, the cells were found to be orientating with one accord, arranging into a muscular at 4 week. In the induction group ,the expression of cTnT and Cx43 was not observed at 1 week. In the co-culture group, the expressions of cTnT and Cx43 were detected at 5th day and increased with time .In the same weeks, the positive rate of cTnT and Cx43 in the co-culture group was higher than the rate of induction group(P<0.01).Conclusion After induced by 5-aza and co-cultured with CMs,the MSCs could transformed into cardiomyocyte-like cells and expressed cTnT and Cx43. The ability of MSCs differentiated into CMs in the co-culture group was stronger than that of the induction group.
    Involvement of caspase-3 activation and PARP-1 inhibition in DMSO induced apoptosis in A549 cells
    Xiao-qin LIU; Feng GUO; Yan-li ZHANG; Hao-lin LIU
    2010, 30(6):  566-569. 
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    Objective To detect whether Poly(ADP-ribose) polymerase-1(PARP-1) is cleaved and therefore inhibited. Methods TUNEL assay and MTT assay of Caspase-3 activity were used to detect apoptosis; and PARP-1 cleavage was detected by western blotting. Results DMSO induced apoptosis in A549 cells and caspase-3 was activated in induced apoptosis, whereas PARP-1 was cleaved into two fragments. Conclusion During DMSO-induced apoptosis in A549 cells, we found that as caspase-3 was activated, PARP-1 was inhibited.
    The effects of oxidative stress on the expression of NKG2D ligands in mouse model
    Cheng-hong LI; Lian-xian CUI; Wei HE; Chi MA
    2010, 30(6):  570-575. 
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    Objective To analyze the effect of oxidative stress on the expression of NKG2D ligands MULT1 and Rae1in murin immune organs and tissues, and study the effect on immune system. Methods We established an oxidative stress mice model through quantitative ozone inhalation. Then we detected the expression of MULT1 and Rae1 in mice thymus, spleen, lymph node and intestine by Real-time PCR and immunohistochemistry after oxidative stress. The cell surface expressions on thymocytes, splenic lymphocytes and intestine intraepithelial lymphocytes (iIELs) were further examined by Flow Cytometry. Results In model mice the expression of MULT1 in thymus was decreased and Rae1 increased; The expression of MULT1 in spleen had no obvious change and Rae1 was increased; The expression of MULT1 in lymph node had no obvious change and Rae1 was decreased; In intestine both the MULT1 and Rae1 expression were up-regulated in epithelial layer. Conclusion There is more complicated regulated mechanism to regulate the expression of MULT1 and Rae1. MULT1 and Rae1 can be used as an biological marker of stress.
    Human AB-serum is a suitable alternative of fetal calf serum for effective expansion of bone marrow mesenchymal stem cells in vitro
    Jian-li HU; Jing LI; Bin CHEN; Chun-jing BIAN; Jie MA; Chun-hua ZHAO
    2010, 30(6):  576-581. 
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    Objective To find a suitable substitute for fetal bovine serum so as to efficient expand mesenchymal stem cells. Methods The biological characteristics of bone marrow-derived mesenchymal stem cells cultured in adult type AB serum,AB-type cord serum and fetal calf serum were compared, including the cell morphology, immunophenotype, cell cycle, growth curve. Results There is no obvious difference between the three. Conclusion These date suggest that type AB serum of healthy adults can be as an alternative of fetal bovine serum for mesenchymal stem cells culture.
    Relationship between Central and Peripheral Obesity and Ventricular Repolarization Inconsistencies of Adults from Heilongjiang Province
    Cheng XU; Xiao-lin YANG; Guang-jin ZHU; Zheng-guo ZHANG
    2010, 30(6):  582-586. 
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    Objective Investigate the relationship between central obesity, which is evaluated by waist circumference (WC) and waist-to-hip ratio (WHR), peripheral obesity, which is measured by body mass index (BMI), and ventricular repolarization inconsistencies. Methods Standard 12-lead ECGs of 2045 adults from Heilongjiang Province were measured with Philips Trim II electrocardiograph. QTc were calculated and corrected by Bazett formula. We used the criterion that QTc>440ms was abnormal. Results QTc and abnormal rates increased with age or obesity indices in both male and female. Females' QTc and abnormal rates were significantly higher than the males'. Logistic regression analysis showed that age, gender, heart rate and systolic blood pressure (SBP) had significant effect on QTc abnormal. BMI, WC and WHR also had significant effect on QTc abnormal. WC was the most significant one. Conclusion Obesity is a significant and independent factor to QTc and abnormal rates. Obesity is significantly correlated with ventricular repolarization inconsistencies. Central obesity has more advantages in weighing adults' ventricular repolarization inconsistencies than peripheral obesity.
    Investigation of the mechanism of PGC-1β regulating transcription of rat ALAS-1
    Yang LIU; Di SHAO; Fu-de FANG; Yong-sheng CHANG
    2010, 30(6):  587-592. 
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    Objective we're trying to determine the cis-element in the regulation of rat ALAS-1 promoter by PGC-1β. Methods Transient transfection with PGC-1β was done in Hep G2, then dual-luciferase reporter assay was performed to investigate fold activity of rat ALAS-1 promoter when over-expressing PGC-1β. Reconstructing ALAS-1 promoter reporters including a series of 5'-deletions and cis-element mutations, dual-luciferase reporter assay was performed to assay NRF-1 binding sites in rat ALAS-1 promoter. We construct adenoviral PGC-1β and adenoviral siRNA of NRF1 and isolate rat primary hepatocytes and assay the effects of PGC-1β on the rat ALAS-1 transcription. Results Overexpression of PGC-1β stimulates the transcription of ALAS-1 in Hep G2 cells, while over-expressing NRF-1 alone had no stimulation. We constructed NRF-1 binding site mutated ALAS-1 promoter, the promoter activity was obviously diminished by overexpression of PGC-1β. PGC-1β promotes the transcription of ALAS-1 in the rat primary hepatocytes. When we use the siRNA to interfere the expression of NRF-1, the stimulating effect of PGC-1β on the ALAS-1 was attenuated. Conclusion PGC-1β promoting the transcription of ALAS-1 is mainly by NRF-1 in Hep G2 cells and rat primary hepatocytes.
    PGC-1α synergizes ERRα to regulate transcription of mouse SHP in human hepatoma cells
    Liu-luan ZHU; Ying CUI; Fu-de FANG; Yong-sheng CHANG
    2010, 30(6):  593-597. 
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    Objective To detect the regulation of PGC-1α and ERRα on mouse SHP transcription in human hepatoma HepG2 cells, and to determine the cis-element involved in the regulation of ERRα on SHP promoter. Methods Transient transfection with ERRα and/or PGC-1α in HepG2 and human embryonic kiney 293A cells, then dual-luciferase reporter assay was performed to investigate fold activity of mouse SHP promoter reporter when overexpressing ERRα and PGC-1α. Reconstructing SHP promoter reporters including a series of 5′-deletions and cis-element mutations, dual-luciferase reporter assay was performed to screen ERRα binding sites in HepG2 cells. Results Coexpression of ERRα and PGC-1α stimulates the transcription of SHP in HepG2 and 293A cells. Five potential ERRα binding sites were identified by analyzing the sequence of SHP promoter. In the dual-luciferase reporter assay with 5′-deletions of promoter, three of five potential elements were demonstrated to be concerned with transcriptional regulation of ERRα. Furthermore, it was demonstrated that two sites of them involve in regulating SHP transcription by ERRα and PGC-1α using mutational promoter luciferase reporter assay, the other one may participate in the basal level transcription of SHP. Conclusion ERRα is a novel pathway that regulates SHP gene transcription in HepG2 cells besides FXR and ERRγ, only when the coactivator PGC-1α is in presence.
    Expression of hCD74 truncated fragment in baculovirus vector system
    Wen-juan SHI; Ding-ding SHAO; Xiang ZHONG; Heng WANG
    2010, 30(6):  598-602. 
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    Objective To establish an expression system of human II CD74 extracellular fragment by insect baculovirus vector expression system in insect cells. Methods hCD74 full extracellular fragment (73-232 aa) and the core area combined with MIF (109-192 aa) were amplified by PCR, and inserted into pFastBacDual vector placed promoter of PPH. Recombinant plasmid was transformed into E. coli DH10Bac and obtained recombinant virus bacmid by site specific transposition. Recombinant bacmid was transfected Sf-21 cell line. The target mRNA transcription were tested by RT-PCR and recombinant protein expression were analyzed by SDS-PAGE and Western-blot. Results The full extracellular fragment (73-232 aa) of hCD74 could be expressed in insect cells and the target protein can be detected using antibodies of His tag and CD74. In addition, it can achieve the maximum expression in cells after 72h infection and inoculation using the virus titer MOI 4. The core area of 109-192 aa fragment detected the mRNA transcription, but not achieve the protein expression. Conclusion hCD74 extracellular fragment was successfully expressed in Sf-21 cell, and optimized the best protein expression conditions.
    Cultivation of endothelial progenitor cells
    Qin WANG; Ke MA; Hong-gang ZHANG; Qiu-ju ZHANG; Rui-jua XIU
    2010, 30(6):  603-608. 
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    Objective To establish a culture system of endothelial progenitor cells from umbilical cord blood. Methods Using density gradient centrifugation to obtain Mononuclear cells,and then cultured them with our labs'culture system.In this study, immunocytochemistry and flow cytometric analysis of CD144,CD146,vWF,CD34 and CD133 were performed. Results The first five days were latent period, cells became adherent, but the number of cells did not increase obviously. At the 6th day, the number of cells came up to 287+45(the average number under 10X10 visual field),and the 9th day up to 282+46(P>0.05).After cultured for 12 days, the cells came into logarithmic phase ,and the number of cells was 805.33+66.61(P<0.05),and continued to incease at day 19, the number of cells was up to 1115+182(P<0.05).Apoptosis took place at day 23, the number of cells decreased to 265+615(P<0.05). Immunocytochemistric analysis indicated that the cells were weakly positive for vWF,CD144 and CD146, and the percentage of CD34 in cobblestone-shaped cells was 88.98%+5.15%(P<0.05), and that of CD133 was 1.20%+1.44%(P<0.05) with flow cytometric analysis. Conclusions The method established in our lab for EPCs culture is effective. The first five days is a latent period, cells become adherent,but do not increase in number; and from day 12 ,the cells come into logarithmic phase. The number of EPCs is increasing at day 19,and then the cells undergo apoptosis at day 23.
    Mutation detection in Coffin-Lowry Syndrome
    Yun WANG; Yan MENG; Yuan-yuan PENG; Zheng WANG; Shi-min ZHAO; Hui-ping SHI; Shang-zhi HUANG
    2010, 30(6):  609-612. 
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    Objective To develop a rapid, reliable and convenient approach for diagnosing Coffin-Lowry Syndrome(CLS). Methods RNA and DNA were extracted from the CLS patients' fresh peripheral blood. The fragments of RPS6KA3 cDNA were amplified by RT-PCR and sequenced. The mutation was verified at the DNA level. The other case that failed to detect the nucleotide change was tested by multiplex ligation-dependent probe amplification (MLPA). Results A two nucleotide deletion (r889_890delAG) in RPS6KA3 gene was detected in the first case, while there was no change in the other boy. The nucleotide sequence of the first case's mother was normal, implying that it was a de novo mutation. Conclusion This was the first time that CLS was diagnosed by molecular method in China, which provided a genetic basis for gene diagnosis and prenatal diagnosis of CLS.
    Regulation of MicroRNA-205 on the Migratory Ability of Normal Human Epidermal Keratinocytes
    Jia YU; Hong-yi FU; Xiao-yan WANG; Qian LIU; Fang WANG; Tao FENG; Jun-wu ZHANG
    2010, 30(6):  613-618. 
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    Objective: We aimed to study the influence of miR-205 on the normal human epidermal keratinocytes (NHEKs) migration by using synthesized antagomir-205 and recombinant lentivirus expressing miR-205, respectively. Methods: Cultured NHEKs were treated with Synthesized antagomir-205. Northern blot analysis was conducted to detect whether endogenous level of miR-205 could be reduced by antagomir-205. Wound healing assay was adopted to further measure the cell migratory ability of NHEKs. Meantime, we also tested its influence on NHEKs by wound healing assay after miR-205 recombinant lentivirus infecting NHEKs. Results: The migratary ability of NHEKs was significantly inhibited by antagomir-205, while ectopic expression of miR-205 promoted NHEKs to migrate. Conclusion: Ectopic expression of miR-205 promoted the normal human epidermal keratinocytes to migrate.
    Expression and significance of HIF-1α and HIF-2α in human gastric cancer
    Neng-lian LI; Guang-ming LI; Ya-li LUO; Li ZHANG; Ya-li SHE; Ya-li WANG; Shu-ming HU
    2010, 30(6):  619-622. 
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    Objective To explore the expression and significance of the hypoxia inducible factor (HIF-1αand HIF-2α) in human gastric cancer.Methods SP immunohistochemical method to detect the expressions of HIF-1α,HIF-2αand VEGF in the tissue samples.Western blot method was also used to measure the expressions of HIF-1αand HIF-2α in the tissue samples.Results Compared with the normal tissue, gastric cancer had significantly higher positive expression rate of HIF-1α(61.5% vs.11.1%), HIF-2α(36.5% vs.0)and VEGF(61.5% vs.0). The expression of HIF-1αwas significantly and positively correlated with lymph node metastasis and VEGF expression. HIF-1αand HIF-2α expression of gastric cancer tissues was significantly higher than that of adjacent non-cancerous gastric mucosa.Conclusion The expression of HIF-1α and HIF-2αmay be important roles for the development and progression of ganstric cancer.
    BMP9 promotes cardiac progenitor cells ameliorate the heart function in the rat model of myocardial infarction
    Lu ZHAO; Yuan CHEN; Biao SHEN; Li-gang ZHOU; Hao WEI; Zhi-rui ZHANG
    2010, 30(6):  623-629. 
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    Objective To test whether cardiac progenitor cells (CPCs) which induced by bone morphogenetic proteins9 (BMP9) can improve the heart function of the rats with myocardial infarction. Methods CPCs were infected by AdBMP9 which express green fluorescent protein. After twenty-four hours, the expression of BMP9 of infected CPCs were examined by semi-quantitative RT-PCR. Then, infected CPCs by AdBMP9 were injected into the infracted heart. The hearts were collected, after postoperative four weeks. The change of CPCs morphology was observed by HE staining and immunofluorescent analysis to investigate the expression of cardiac-specific structural proteins alpha myosin heavy chain(α-MHC) andα-Actin of the implanted CPCs .The cardiac infarct size was detected by Masson staining. Heart function was examined by echocardiography, in preoperative and postoperative four weeks. Result After twenty-four hours, the expression of BMP9 of CPCs infected by AdBMP9 improved significantly(P﹤0.05). The result of HE staining and immunofluorescent showed that the grafted-CPCs could fuse with the normal cardiomyocytes and expressed the cardiac-specific proteinsα-MHC and α-Actin, in the four weeks after transplantation. But the expression of cardiac-specific structural proteins is lower than the normal cardiac tissue(P﹤0.05). In the postoperative four weeks, the implanted CPCs can reduce the sizes of infracted obviously(P﹤0.05)and which can be promoted by BMP9(P﹤0.01).The result of heart function is similar with the Masson staining. Conclusion CPCs induced by BMP9 can improve the heart function of the rats with myocardial infarction.
    Activation of Akt/mTOR siganling pathway in the rat hippocampus following kainate injection
    Jin-lin FANG; Chun-chun WU; Xiao-hong SUN; Qun-yuan XU; De-yi DUAN
    2010, 30(6):  630-634. 
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    Objective To explore roles of Akt/mTOR signal pathway activation and HIF1α expression in the lesioned hippocampus following KA treatment. Methods Adult Sprague Dawley (SD) rats were radomly divided into three groups, two of them received intracerebroventricular (ICV) injection and one served as the sham group. After rats were anaesthetizeed and a skull hole drilled, ICV injection of KA (1.0 ?g) (KA group), or the same volume of nomal saline (NS group) were made. Sham group rats underwent skull hole drilling with no injection. 8 h, 16 h, 1 d, 3 d and 5 d after KA or NS administration, expression of Akt/phospho-Akt and mTOR/p-mTOR was examined by Western blot, and HIF1α expression was evaluated by immunohistochemistry. Results Nissl staining revealed an initial neuronal death in the hippocampus CA3 region 1 d after KA injection and in the CA1 region at 5 d. Western blot indicated that Akt was activated at 16 h and persisted for at least 5 d, an initial activation of mTOR started at 1 d, peaked at 3 d and then returned to baseline level. Expression of HIF1 was increased significantly at 1 d in the hippocampus CA3 region and at 3 d in the CA1 region. No significant changes were observed in both the NS and the sham rats. Conclusion Akt/mTOR signaling pathway was activated and expression of HIF1α was increased in KA-lesioned rats and it maybe modulate the survival of hippocampal neurons.
    Expression and significance of ER、PR in papillary thyroid carcinoma
    Lu ZHANG; Yong-liang SUN; Shi-ping CHEN; Hong-yan DONG; Yue-wu LIU
    2010, 30(6):  635-639. 
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    Objective The aim of this study was to determine the expression of ER、PR in human papillary thyroid carcinoma(PTC), and to explore their correlation with the clinical features of thyroid carcinoma. These features as following: sex、age、tumor size、capsular invasion、lymph nodes metastasis and tumor grade malignancy. Methods Taking 69 samples of fresh papillary thyroid carcinoma (PTC) and 7 samples of nodular goiter from surgery, the expression of ER、PR were detected in human papillary thyroid carcinoma by immunohistochemistry. And the correlation of the expression of ER / PR and the biological characteristics of tumor was analyzed. Results In PTC samples, ER and PR immunoreactivity was detected for 52.17% and 46.38% ,respectively. There was not the expression of ER、PR in nodular goiter. The expression of ER and PR was not associated with sex、tumor size and tumor grade malignancy,but the expression of PR was positively correlated with capsular invasion and lymph nodes metastasis (P<0.05),the expression of ER was negatively associated with age. Conclusion The expression of ER and PR was higher in PTC than nodular goiter. The expression of PR in PTC may be a sign of capsular invasion and lymph nodes metastasis, the expression of ER decreased as the patients grow older..
    The primary culture of myocadial microvascular endothelial cells of rats in vitro
    Na LI; Ming-zheng PAN; Hong ZHANG
    2010, 30(6):  640-643. 
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    Objective To improve methods of primary culturing rats myocadial microvascular endothelial cells in vitro,and observing the growth feature. Methods Enzyme digestion cultivation of cadiac tissue was used to obtain the primary cells.In the subculturing, the cell cultures were purified by different speeds of attachment and sub-cell cloning method. The purified cells were identified as endothelial cells by factor Ⅷ and CD31 related antigen ,the two markers for endothelial cells,and observing the growth feature by inverted phase contrast microscope. Results The MMVECs were cultured and purified successfully which can be used in the related researches. Conclusion  The protocol of culturing MMVECs was succeeded, and it is reliable.
    Diagnosis and treatment for primary adult renal sarcoma
    Zhong-ming HUANG; Bing-bing SHI; Han-zhong LI; Zhi-gang JI
    2010, 30(6):  644-646. 
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    Objectives To investigate the clinical diagnosis, treatment and prognosis of primary adult renal sarcoma. Methods 1654 cases of adult renal tumors were treated from 1985 to 2009, 17 cases of adult primary renal sarcoma in all of the tumors were analyzed retrospectively. Results The first symptom of 10 cases in all renal sarcomas was lumbar and abdominal mass. All 17 patients accepted radical nephrorectomy, with the pathological diagnosis of leiomyosarcoma (7 cases), rhabdomyosarcoma (2 cases), malignant fibrous histocytoma (2 cases), low-differentiated sarcoma (2 cases), and liposarcoma, fibrosarcoma, embryonic sarcoma and leiomyosarcoma coexist with chromophobe renal cell carcinoma for each one. One with tumor thrombosis in inferior vena cava died on table for pulmonary artery thrombosis. 15 cases were regularly followed up; one still lives without recurrence for 9 months, the other 14 cases died in 5 to 60 months with the average survival duration of 18 months. Average survival of leiomyosarcoma group was 28(11~60) months, and 2 case of malignant fibrous histocytoma died in 4 and 8 months respectively. Conclusion The primary renal sarcoma has the common clinical symptom similar with advanced renal cell carcinoma, and poor prognosis. Stage of tumor, pathologic type and no post-operational remained tumor are the major prognostic factors.
    Observation on effect of diaphragm biofeedback training combined with proton pump inhibitor in therapy of gastroesophageal reflux related cough
    Huan WU; Mei-yun KE; Xiao-hong SUN; Zhi-feng WANG; Xiao-hong LIU; Xiu-cai FANG; Li-ming ZHU
    2010, 30(6):  647-650. 
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    Objective To observe the effect of diaphragm biofeedback training( DBT) combined with proton pump inhibitor (PPI) to treat gastroesophageal reflux (GER) related cough. Methods 11 patients with GER related cough were investigated. After completing questionnaire, they were taught how to practice DBT daily at home and record diary, meanwhile taking PPI (Rabeprazole 10mg, twice daily). We made follow-up every 2 weeks and asked them to complete health-related quality of life questionnaire (SF-36) at the end of the 8th week, and made a telephone call to them at the end of the 12th week. Results Total reflux symptom score(TRSS) and score of cough(SC) could be reduced by 50% after therapy of DBT combined with PPI. Symptoms remained stable in 8 weeks, and score of role limitations due to physical health, vitality, mental health, social function, body pain, general health increased significantly (P <0.05). At the end of the 12th week, symptoms remained stable while dosage of PPI decreased. Conclusions DBT combined with PPI can significantly improve patients' symptoms and quality of life in a short term, which brings forward a new therapeutic approach for patients of GER related cough.
    Clinical Use of New Intravenous Electrocardiogram (ECG) Guided Central Venous Catheterization
    Zhong-huang XU; Gang TAN; Hong-zhi REN; Yu-guang HUANG; Ai-lun LUO
    2010, 30(6):  651-653. 
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    Objective To evaluate the effectiveness and safety of new intravenous Electrocardiogram (ECG) guidance to confirm accurate positioning of central venous catheters (CVC). Methods 80 patients were divided into two groups by the CVC pathway: internal jugular vein group(Group 1, n=40) and right subclavian vein group (G2, n=40), and CVC placed using right-atrial ECG to guide catheter tip positioning. The depth of the CVC, central venous pressure (CVP),and CVC related complications were recorded during the observation. RESULTS The frequency of emergence of the typical P-wave were 98.7% (77/78). The depth of the catheter placed with the ECG guidance was significantly shorter than the estimated value by the traditional formula. Conclusions ECG guidance allows for more accurate CVC placement, and should be considered to increase patient safety and reduce costs associated with repositioning procedures.
    The diagnosis and treatment of silent pulmonary thromboembolism in post-neurosurgical-operative patients
    Jun-ji WEI; Ren-zhi WANG; Ya-hong GONG; Wen-bin MA; Gui-lin LI; Ming FENG; Wan-chen DOU; Jun GAO
    2010, 30(6):  654-657. 
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    Objective To study the silent pulmonary thromboembolism (SPE) and improve the management of diagnosis and treatment to the patients. Methods To analyze the clinical data of 2 cases who were diagnosed as SPE. Based on screening of D-dimer, ultrasound imaging and CTPA, the patients were diagnosed and treated initialy with low-dose heparin or low-molecular-weight heparin and then with warfarin. 1of the 2 cases had implanted permanent inferior vena cava filters before Anticoagulation. Results One of the result of D-dimer is 573μg/L(156μg/L after treatment),the other is 246μg/L(50μg/L after treatment). The ultrasound imaging showed the low extremities vein thrombosis and the CTPA confirmed the pulmonary thromboembolism. Both of them discharged after re-examinations with CTPA which showed the improvement of pulmonary thromboembolism. Conclusion The neurosurgeons should pay much attention on the high risks of SPE and screen such patients ealier. The critical thing is to make a program to prevent and treat SPE peri-operatively.
    The dose selection of progesterone therapy for endometrial atypical hyperplasia
    Xiao-li WEI; Yuan-zheng ZHOU; Ai-jun SUN; Ji LIN
    2010, 30(6):  658-661. 
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    Objective To explore the outcomes of progesterone therapy for vary degrees of endometrial atypical hyperplasia, and the dose of progestin for vary degrees of endometrial atypical hyperplasia. Methods Twenty-five patients with endometrial atypical hyperplasia were treated with high dose of progestin from Jan 2002 to Jun 2006. The effect of treatment and the dose of progestin for vary degrees of endometrial atypical hyperplasia were analyzed respectively. Results 1. Nineteen cases achieved regressions after treated for 3~36 months;Four was partial remission ; Tow had persistent lesions.2. The range of dose of progestin for the mild and moderate endometrial atypical hyperplasia was 125~250 mg/d ,and the rate of complete remission was 87.5%(7/8)and 66.7%(6/9),and the remission time was from 15 to 3 month with increasing dose of progesterone.The range of dosage of progestin for the severe was 250~500 mg/d and the rate was 75%(6/8). Conclusions For progesterone treatment of vary degrees of endometrial atypical hyperplasia, with increasing dose of progesterone ,its efficiency and remission rates increased and the remission time minimized.
    Analysis of human cytomegalovirus diseases in 38 case of healthy adult
    Chang-ting MENG; Xiao-qing LIU; Bao-tong ZHOU; Zhi-feng QIU; Guo-hua DENG
    2010, 30(6):  662-665. 
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    Objective To analyze the features of human cytomegalovirus(CMV) infection in adults without obvious organic disease. Methods Restrospective analyze 38 cases of CMV infection in healthy adult inpatients without obvious organic disease from 1995 to 2008 in PUMC hospital,review their clinical manifestation, labortary test, treatment and prognosis. Results The features of CMV infection in adults without obvious organic disease are varied. Most common clinical feature is fever(68.4%), other include enlarged lymph nudes(28.9%), liver(34.2%) and spleen(44.7%) and atypical lymphocytes in peripherial blood. In acute phase of diseases, T cell subset showed reversed CD4/CD8 cell ratio, significantly increased amount of subset of CD8+ T cell(CD8+CD38+)(732±760 vs. 271114, P=0.024). Decreased NK cell was also found(125±146 vs. 371±196, P=0.00). Prognosis is poor in patients with mutiple organs dysfunction, Ganciclovir is effective. Conclusion: CMV diseases in healthy adult are with varied features and may cause severe diseases, which should be pay great attention.
    The relationship of the metabolites of kynurenine and the serum levels of hsCRP in patients with cerebral hemorrhage
    Xi-ming MO; Ai-guo TANG; Zhong-yuan XIANG; Xi-bo LUO
    2010, 30(6):  666-667. 
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    Combined G-CSF and simvastatin on cardiac apoptosis after AMI of Sprague-Dawley rats
    Fang-xin TA; Feng-qin ZHAO
    2010, 30(6):  668-669. 
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    The interferinging effects of the Valsartan on airway remodeling in asthmatic model in mice
    Hong-mei PIAO; Chang-shan AN; Qiu-hong SONG
    2010, 30(6):  670-672. 
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