Abstract: Objective To analyze the effect of oxidative stress on the expression of NKG2D ligands MULT1 and Rae1in murin immune organs and tissues, and study the effect on immune system. Methods We established an oxidative stress mice model through quantitative ozone inhalation. Then we detected the expression of MULT1 and Rae1 in mice thymus, spleen, lymph node and intestine by Real-time PCR and immunohistochemistry after oxidative stress. The cell surface expressions on thymocytes, splenic lymphocytes and intestine intraepithelial lymphocytes (iIELs) were further examined by Flow Cytometry. Results In model mice the expression of MULT1 in thymus was decreased and Rae1 increased; The expression of MULT1 in spleen had no obvious change and Rae1 was increased; The expression of MULT1 in lymph node had no obvious change and Rae1 was decreased; In intestine both the MULT1 and Rae1 expression were up-regulated in epithelial layer. Conclusion There is more complicated regulated mechanism to regulate the expression of MULT1 and Rae1. MULT1 and Rae1 can be used as an biological marker of stress.

Key words: oxidative stress, Rae1, MULT1, NKG2D, expression