Basic & Clinical Medicine ›› 2010, Vol. 30 ›› Issue (6): 613-618.

• 研究论文 • Previous Articles     Next Articles

Regulation of MicroRNA-205 on the Migratory Ability of Normal Human Epidermal Keratinocytes

Jia YU, Hong-yi FU, Xiao-yan WANG, Qian LIU, Fang WANG, Tao FENG, Jun-wu ZHANG   

  1. Institute of Basic Medical Sciences, CAMS&PUMC Chongqing Medical University
  • Received:2010-02-26 Revised:2010-04-09 Online:2010-06-05 Published:2010-06-05
  • Contact: Jia YU,

Abstract: Objective: We aimed to study the influence of miR-205 on the normal human epidermal keratinocytes (NHEKs) migration by using synthesized antagomir-205 and recombinant lentivirus expressing miR-205, respectively. Methods: Cultured NHEKs were treated with Synthesized antagomir-205. Northern blot analysis was conducted to detect whether endogenous level of miR-205 could be reduced by antagomir-205. Wound healing assay was adopted to further measure the cell migratory ability of NHEKs. Meantime, we also tested its influence on NHEKs by wound healing assay after miR-205 recombinant lentivirus infecting NHEKs. Results: The migratary ability of NHEKs was significantly inhibited by antagomir-205, while ectopic expression of miR-205 promoted NHEKs to migrate. Conclusion: Ectopic expression of miR-205 promoted the normal human epidermal keratinocytes to migrate.

Key words: microRNA, epithelial cells, cell migration