Table of Content

    05 May 2010, Volume 30 Issue 5
    Testosterone induces cardiomyocyte hypertrophy in rats and upregulates the expression of ERK1/2
    Ting-huai WANG; Yan XU; Hai-mei LIU; Yu-hong CUI; Jin-wen XU; Ping JIANG; Xiao-dong FU
    2010, 30(5):  449-453. 
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    Objective To explore the role of ERK1/2 protein in the signal transduction passway of testosterone(T) in development of myocardial hypertrophy. Methods Myocardial cells were isolated from ventricles of 1~3-day-old neonate rats purifed by a culture method based on Simpson. Neonate rat cardiomyocyte hypertrophic responses were assayed by measuring protein content, protein synthesis rate and cell surface area. Expression of protein ERK1/2 were detected by western blotting. Results Cell protein content, 3H-leucine (3H-leu) incorporation and cell surface area increased by treating of cardiomyocytes with T (10-10~10-6mol/L) for 24h. The maxium effect was observed at the concentration of 10-8mol/L. The increase of cell protein content induced by T could be inhibited by pretreating with Flutamide (10-5mol/L) for 2h, while there had no effect on cardiomyocytes pretreating with Flutamide alone. The increase of 3H-leu incorporation induced by T was largely blocked by PD98059(50μmol/L). Expression of ERK1/2 was upregulated significantly by treating with testosterone for 24h at the level of 10-8mol/L. The increased expression of ERK1/2 induced by T was reversed by pretreating with Flutamide(10-5mol/L) for 2h. Conclusion T with physio-concentration could induce cardiomyocyte hypertrophy and this effect was possibly mediated through the activation of ERK1/2 signalling. During this procession, T upregulated the protein expression of ERK1/2 mediated by androgen receptor.
    Primary Investigation of the Therapeutic Effect of Stem Cell Transplantation in Patients with Leukemia based on changes in Haptoglobin
    Pei-xuan BI; Yang ZENG; Zhi-li LI; Chun-hua ZHAO
    2010, 30(5):  454-458. 
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    Objective To explore the effects of different approaches of stem cell therapy in patients with leukemia on haptoglobin (Hp). Methods Four patients treated by mesenchymal stem cells (MSC) with hematopoietic stem cells cotransplantation (HSCT), and two patients treated by hematopoietic stem cell transplantation (HSCT) were as objects of the study. Haptoglobin in the plasma, collected from different therapeutic stages, of six patients was separated by two-dimensional gel electrophoresis (2-DE), followed by the identification with MALDI-TOF/TOF mass spectrometry. Results The abundance of haptoglobin alpha and beta chains with different modification decreased along with an increasing therapeutic time window. This tendency was more significant in HSCT group. Conclusion The haptoglobin may be a potential biomarker for monitoring prognosis in patients with leukemia treated by stem cell transplantation.
    Effect of different IL-2 dosages on the proliferation and phenotype of human peripheral blood γδT cells
    Ning KANG; Dan WU; Yu HU; Lian-xian CUI; Wei HE
    2010, 30(5):  459-465. 
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    Objective To study the effect of interleukin 2 (IL-2) , which is within the clinical dose range, on the proliferation of human peripheral blood T cells, with special emphasis on the number and functional phenotype of γδT cells. Methods Human peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation and cultured for 2 weeks at different IL-2 concentrations. Ratio and phenotype of different T cell subpopulations before and after in vitro expansion were explored by immunofluorescence staining. Cell number was estimated by trypan blue staining and cell counting. Results Within the four functional phenotypes of Vδ1 as well as Vδ2 γδT cells, CD27+ cells (including CD27+CD45RA+ and CD27+CD45RA- subsets) could express lymphoid tissue homing receptor CCR7, whereas CD27- cells (including CD27-CD45RA+ and CD27-CD45RA- subsets) had the peripheral tissue homing potential. All the studied γδT functional subsets had the expression of activity related receptors, and the ability of a rapid production of various amount of cytotoxicity related effectors following mitogen stimulation. Although IL-2 at high concentration suppressed the proliferation of CD4 T cells, it could promote the proliferation of γδT cells. The proliferated γδT cells were mainly CD27-CD45RA- effector cells. Discussion IL-2 within the clinical dose range could promote the proliferation of human peripheral blood γδT cells, which might have important biological significance in IL-2 based anti-tumor therapy.
    Inhibitory effect of CAI on iNOS expression and NF-κB activation degradation in rat peritoneal macrophages
    Ru ZHENG; Xiao-jian HAO; Lei GUO; Juan LI; Xiao-li YU; Cai-ying YE; De-chang ZHANG
    2010, 30(5):  466-470. 
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    Objective This study is designed to explore the regulations of carboxyamidotriazole (CAI) on kinds of inflammatory factors in vitro and its underlying mechanisms. Method Peritoneal macrophages were incubated with different concentrations (5-40 μmol/L), and then cell viabilities were evaluated by MTT assay. Cells were pretreated with CAI (5-40 μmol/L), LPS was then added, and cells were incubated for 18 h. NO and TNF-α levels were determined with Griess reagent and ELISA kit, respectively. The iNOS expression and NF-κB activation were detected by Western blot method. Result The rat peritoneal macrophage viablilties were not affected at the concentrations of CAI used. CAI (5-40 μmol/L) was found to reduce NO (p< 0.01, p< 0.001) and TNF-α production (p< 0.05, p< 0.01) in a dose-dependent manner. CAI was also found to inhibit the LPS-induced expression of iNOS and degradation of IκBα in rat peritoneal macrophages. Conclusion The findings of the present study suggest that CAI has suppressive effect on iNOS expression, and this inhibitory effect of CAI was found to be associated with NF-κB inactivation via the blockade of IκBα phosphorylation.
    Complement mediated killing of human glomerular mesangial cell by Fcα/μ Receptor
    Lian SHEN; Xiao-yan WANG; Qing ZHAO; Wei ZHANG
    2010, 30(5):  471-475. 
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    Objective To study whether Fcα/μR can mediate complement killing of human glomerular mesangial cells. Methods The Fcα/μR cDNA contained plasmid, pcDNA3.1-Fcα/μR was transfected into a human glomerular mesangial cell line, NHMC. Fcα/μR expression was detected by Western blotting and laser scanning confocal microscopic analysis. Binding of IgM-immune complexes (IgM-IC) to the Fcα/μR on cell membrane was detected by flowcytometry and laser scanning confocal microscopyic analysis. Killing of cells by complement was analyzed by Trypan blue exclusion assay. Results NHMC cells transfected with Fcα/μR could bind IgM and IgM-IC. After treatment with complement, added IgM-IC, the death rate of pcDNA3.1-Fcα/μR transfected cell was significant higher than the control groups of wild type cell, pcDNA3.1 transfected cell and the pcDNA3.1-Fcα/μR transfected cell without IgM-IC. Conclusion IgM-IC can be bound by Fcα/μR expressed NHMC cells and mediate complement killing of the cells.
    Upregulated expression of IGF-2R induced by hypoxia and serum deprivation in neonatal rat cardiomyocytes in vitro
    Xiu-fang ZHANG; Qiang SHI; Chuan-jue CUI; Jun LI; Ying-jie WEI; Sheng-shou HU
    2010, 30(5):  476-479. 
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    Objective To investigate the expression of insulin-like growth factor 2 receptor (IGF-2R) induced by hypoxia and serum deprivation (H/SD) in cultured neonatal rat cardiomyocytes in vitro. Methods Cultured neonatal rat cardiomyocytes were randomly divided into control group and H/SD group. Expression of IGF-2R mRNA was measured by RT-PCR and real time RT-PCR. Level of IGF-2R protein was measured by enzyme-linked immunosorbent assay (ELISA). Results Compared with control group, the expression of IGF-2R mRNA increased significantly (P<0.01) in cardiomyocytes cultured in H/SD condition for 12h and 24h, and IGF-2R protein was upregulated in cardiomyocytes cultured in H/SD condition for 24h(P<0.05). Conclusion H/SD could induce IGF-2R expression, and IGF-2R may involve in the mechanism of cardiomyocyte damage induced by ischemia.
    Lung function reference values and prediction equations in children and adolescents of the Han nationality in Heilongjiang province
    Kui FENG; Li CHEN; Shao-mei HAN; Guang-jin ZHU
    2010, 30(5):  480-486. 
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    Objective  To establish Lung function reference values and prediction equations in children and adolescents of the Han nationality in Heilongjiang province. Methods  A survey in 588 healthy children and adolescents (292 males , 296 females) aged 10 to 18 years old was carried out in Heilongjiang province in 2008. Eight flow-volume tests parameters [e. g. forced vital capacity (FVC) , forced expiratory volume at one second(FEV1)] were measured. Stepwise multiple regression was carried out to establish prediction equations for the parameters mentioned above. The difference between observed values and the predicted values derived from current authors' and other related prediction equations were compared. Results FVC and FEV1, were found, regardless of sex, to tend to go up with the increase of age( P all <0.001). Beginning from the age of 14, male FVC and FEV1 became significantly higher than those of the female( P < 0. 001), the period of sudden increase of the male FVC and FEV1 taking place during the age of 13-14, while that of the female taking place in the age of 12-13, one age bracket earlier than the male. All lung volumes and flow rates, were found, regardless of sex, to tend to go up with the increase of age, height and weight(P all< 0.001). The correlation among lung function parameters was higher in height than weight and age. The regression equations of lung function were established. By comparison with the equations derived from our study and other authors' study, it was found that the difference between measured data and predicted values from other authors were higher than those from ours. Conclusion Reference values and prediction equations for forced expiratory lung function applicable for children and adolescents of the Han nationality in Heilongjiang province were established.
    Visualization of serotonin 1A receptor trafficking in neuron-like PC12 cells
    Yan-yan JIN; Qiong LV; Zhu-qing YAN; Er-jing GAO; Chun-li ZHAO; Jin-lu ZHANG; Zhi-qing XU
    2010, 30(5):  487-491. 
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    Objective In order to get a better understanding of the mechanisms of trafficking and signaling of serotonin 1A receptor (5-HT1A),we fused it with the enhanced green fluorescent protein (EGFP) to study its spatiotemporal distribution in living cells. Methods The mouse 5-HT1 A gene amplified by RT-PCR was recombined into pEGFP-N1 vector and the EGFP coding sequence was located in-frame at the C-terminal end of the 5-HT1A receptor. The 5-HT1A-EGFP was transfected into neuron-like PC12 cells as well as HEK293 using cationic liposomes. A stable 5-HT1A-EGFP expressing PC12 cell clone was isolated under fluorescence microscopy. The transfected cells were visualized using a live cell confocal microscopy system , meanwhile the mobility of 5-HT1A-EGFP was monitored by live measurements and fluorescence recovery after photobleaching. Results The 5-HT1A gene was identity with the published gene sequence NM_008308.4 and a 5-HT1A-EGFP fusion construct was created. After stable transfection of the plasimd into a PC12 cell line and analysis with a confocal laser scanning microscope, the EGFP-tagged 5-HT1A was predominantly associated with the plasma membrane, but some intracellular vesicles in the perinuclear region also contained the fusion protein. The predominant localization of 5-HT1A-EGFP at the plasma membrane was confirmed in transiently transfected HEK293 cells. Bleached fluorescence was partialy recovered in 100 seconds, indicating that the 5-HT1A-EGFP was mobiled on the membrane. Conclusion Spatiotemporal distribution and mobility of 5-HT1A tagged with EGFP can be monitored in the 5-HT1A-EGFP stable PC12 cell line, which could be an excellent neuron-like experimental cell model in research on 5-HT1A trafficking and signaling.
    Optimization of refolding of recombinant human endostatin
    Li-xi ZHAO; Yong-ping JIANG
    2010, 30(5):  492-495. 
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    Objective To optimize the conditions for enhancing the refolding of a novel recombinant human (rh) endostatin and test the biological activities of refolded endostatin. Methods The partial purified inclusion bodies of rh-endostatin were dissolved with 6mol/L Guanidine-HCl followed by combination of dilution and dialysis of the dissolved endostatin. The refolded endostatin was then purified by cation-exchange chromatography. The biological activities of purified rh-endostatin were assessed by using endostatin-specific monoclonal antibody and chick embryo chorioallantoic membrane assay. Results The 63% refolding yield was achieved after optimizing the refolding conditions. The purified endostatin was reacted with specific anti-endostatin monoclonal antibody and showed significant inhibition of angiogenesis in chick embryo chorioallantoic membrane assay. Conclusion The method of highest refolding yield of human endostatin so far was developed. This optimized method will greatly increase the application of this novel human endostatin to preclinical and clinical studies.
    The interaction between C5aR,C5L2 and chemotaxis inhibitory protein secreted by Staphylococcus aureus
    Zhen-jia LIU; Guan-hua DU; Li-li GONG; Jin-ming GAO
    2010, 30(5):  496-499. 
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    Objective To investigate the physical association between G protein-coupled receptors (GPCR) including C5a receptor (C5aR), C5a Like Receptor 2 (C5L2) and chemotaxis inhibitory protein (CHIPs) secreted by Staphylococcus aureus. Methods The purified CHIPs was incubated with HEK 293T cells overexpressing C5aR, C5L2 and C-X-C chemokine receptor type 3 (CXCR3). Binding signal was detected by Western-blotting. Results HEK 293T cells overexpressing C5aR can efficiently bind to CHIPs. No apparent association between CHIPs and C5L2 or CXCR3 was detected. Conclusion C5aR not C5L2 is a membrane receptor for binding CHIPs, It suggesting a difference between C5aR and C5L2 in association with binding CHIPs.
    Effect of HPV E6 siRNA on the Proliferation and Chemotherapy Sensitivity in Cervical Cancer HeLa Cells
    Jing-hua LI; Wei-juan WANG; Wan-li GAO; Lei GUO; Li-min FENG
    2010, 30(5):  500-504. 
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    Objective To elucidates the effects of HPV18 E6 siRNA which targets human papillomavirus (HPV) 18 E6 gene on the proliferative activity of HeLa cells and chemotherapy sensitivity. Methods HPV18 E6 expressions of HeLa cells were inhibited by siRNA interference, the changes of P53 and P21 proteins expression level were measured by Western blot. MTT assay was used to detected proliferative activity and sensitivity to paclitaxel liposome in HeLa cells. Results After inhibition of E6 expression, P53 and P21 proteins level increased and the growth of HeLa cells decreased(P<0.01). The inhibition rate of HeLa was markedly increased after transfection of HPV18 E6 siRNA and paclitaxel liposome. Conclusion HPV18 E6 siRNA can effectively silence gene expression of E6 and inhibit cell proliferation in HeLa cells. Cervical cancer HeLa cells are more sensitive to combine HPV18 E6 siRNA with paclitaxel liposome than that of control groups.
    Expression and Regulation of HES1 in Sperm with Low Motility of Asthenospermia Patients
    Yan LI; Liu WEN; De-yu CHEN
    2010, 30(5):  505-509. 
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    Objective: To analyze the expression and regulation of HES1 in sperm with low motility. Methods: Thirty semen samples from asthenospermia patients and 20 semen samples from healthy and fertile adult men were collected, and total RNAs were extracted to produce cDNAs probes. Hybridization with Phalanx OneArrayTM contained 30 968 probes was carried out after the labeled cDNAs were purified by PCR Product Purification Kit. Realtime RT-PCR was used to analyze the expression of hsa-miR-487a and hsa-miR-193b; the expression of the target genes of hsa-miR-487a and hsa-miR-193b were searched from gene-expression profiles in asthenospermia patients' sperm. Results: The expression level of HES1 in low motility sperm was up-regulated. The expression level of hsa-miR-193b in low motility sperm was 2.19 fold of that in high motility sperm, hsa-miR-487a was 0.43 fold. Conclusion: The expression level of HES1 in low motility sperm was up-regulated. Hsa-miR-487a and hsa-miR-193b might affect on the expression of HES1 and regulat sperm motility.
    Relation between Vagus Nerves and Remodeling of Gap Junction in Superior Vena Cava Myosleeve in Canine with Atrial Fibrillation
    Yun LING; Guo-qiang ZHONG; Jin-yi LI; Yan HE; Jing-chang ZHANG; Hong-xing SONG; Ri-xin XIONG
    2010, 30(5):  510-514. 
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    Objective To investigate the relationship between cardiac vagus nerves and changes of connexins(Cx) and intracellular gap junction(GJ) distribution pattern in superior vena cava(SVC) myosleeve in canine with atrial fibrillation(AF). Methods Twenty four hybrid dogs were divided into sham operation group(Sham group,n=8),SVC-AO fat pad removed group(RM group,n=8) and SVC-AO fat pad reserved group(RS group,n=8).In RM group and RS group, right atrial pacing was performed at a frequency of 500-650/min for 6 weeks to establish AF model. AF was induced by programmed stimulation or burst stimulation of atrial pacing. The expression and distribution of Cx40, Cx43 in the SVC myosleeve tissue in three groups were analyzed by immunofluorescence staining. Transmission electron microscope was used to observe the ultrastructural organization of gap junction(GJ). Results The rate of inducing sustained AF(>15minutes) in RS group was significantly higher than that in RM group(P<0.01). The expression of Cx40, Cx43 in the SVC myosleeve in sham group and RS group were significantly higher than that in RM group(P<0.05). Furthermore, the expression of Cx40, Cx43 in RS group were obviously higher than that in sham group(P<0.05). The ratio of end-to-end to side-to-side in RS group was lower than that in Sham group and RM group. Comparing with RM group, the channel of GJ became shorter and wider in RS group(P<0.05). Sarcomere was dissolved and mitochondrion showed vacuole degeneration in RS group. Conclusions The remodeling of Cx40, Cx43 in SVC myosleeve tissue may be mediated by vagus nerves. It is conducive to the maintenance and stability of AF. However, this effect can be weakened by removing SVC-AO fat pad of canine.
    AGEs Induced The Expression Of INOS In Cardiac Microvascular Endothelial Cells in Rat
    Yong-xia CHENG; Gui-bo LIU; Su-fen GUO; Xiang-hong YANG
    2010, 30(5):  515-519. 
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    Objective To observe the effect of the advanced glycation end products (AGEs) on the iNOS expression in cardiac microvascular endothelial cells. Methods Cultured the cardiac microvascular endothelial cells in vitro. After AGEs of the different dose(50~200mg/L)and different times(0~24h) played the role on the cells, we determined the NO generation and iNOS protein expression of all groups. Results NO generation and iNOS protein expression increased with the AGEs -dose increasing and treatment time prolong. Statistical analysis of the results have significantly differences. Conclusin These results demonstrated that AGEs could induce iNOS to produce toxic NO in cardiac microvascular endothelial cells ,then the diabetic cardiomyopathy would occure on clinical.
    The expression of microRNA130a during chondrogenic differentiation of rat bone mesenchymal stem cells
    Jin-mei SU; Ye JIN; Qiang QU; Feng-chun ZHANG; Fu-lin TANG
    2010, 30(5):  520-523. 
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    Objective To evaluate the role of microRNA130a during chondrogenic differentiation of rat bone mesenchymal stromal cells (BMSCs). Methods BMSCs were induced to differentiate into chondrocytes by transforming growth factor-β1(TGF-β1) in vitro, immunofluorescence and immunohistochemistry were performed to evaluate MSCs differentiation. Real-time reverse transcription polymerase chain reaction was performed to analyze microRNA130a expression at different time points (before induced culture, 7 days later in induced cultures and 7 days later in non-induced culture). Results We found microRNA130a was down-modulated during chondrogenesis after BMSCs had cultured in the present of TGF-β1 for 7 days(P<0.05). Conclusion These findings show that, during the early stage of BMSC chondrogenic differentiation, mciroRNA130a expression was specifically repressed, suggesting a role in differentiation of rat bone mesenchymal stromal cells.
    The effect of co-inoculating human lymphatic endothelial cells on growth and metastasis of breast cancer cell and osteosarcoma cell in nude mice
    Hong-lin LIU; Lian-qiu WU; Li-ya YE; Jin-ning LOU; Wen-jian ZHANG
    2010, 30(5):  524-529. 
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    Objective To compare growth and metastasis of breast cancer cell and osteosarcoma cell influenced by co-inoculating human lymphatic endothelial cell (HLyEC), and to analyze its mechanisms. Methods HLyEC was isolated and purified from human lymph node by magnetic beads coated with antibody against human VEGFR3. Human breast cancer cell line (MDA-MB-435) or human osteosarcoma cell line (MG-63) was inoculated alone or co-inoculated with HLyEC subcutaneously into nude mice. Weight of tumors were detected; the peri-tumor lymphatic vessel density was shown by Evans blue, the intra-tumor lymphatic vessel density and origin of intra-tumor lymphatic endothelial cell were evaluated by immunohistochemistry for human and mouse PDPN. And the metastasis clones on lungs was counted. The effect of MDA-MB-435 and MG-63 condition medium on proliferation of HLyEC was evaluated by MTT assay. Results The growth of MDA-MB-435 but not MG-63 was promoted by co-inoculating HLyEC with much higher weight of tumor. The peri-tumor and intra-tumor lymphatic vessel density of MDA-MB-435 were increased by co-inoculating HLyEC, both hPDPN- and mPDPN-positive lymphatic vessels were found in MDA-MB-435 co-inoculating with HLyEC group. But co-inoculating HLyEC has no effect on lymphatic vessels density of MG-63. Neither hPDPN- nor mPDPN-positive lymphatic vessels were found in MG-63 tumor. And many metastasis clones were visible on lungs only at MDA-MB-435 co-inoculating with HLyEC group. The result of MTT assay showed that the proliferation of HLyEC was increased by condition medium of MDA-MB-435 but not MG-63. Conclusion Breast cancer has active lymphangiogenesis which might contribute to its growth and metastasis.
    Compound Betamethasone enhances the expression of olfactory marker protein in the injured olfactory mucosa of mice by influenza virus
    Gui-lian WAN; Dao-feng NI; Jing GUAN
    2010, 30(5):  530-533. 
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    Objective To investigate the effects of Compound Betamethasone on the expression of olfactory marker protein(OMP) in murine injured olfactory mucosa by influenza virus. Method An animal model was produced by intranasal application of influenza virus to mice. Compound Betamethasone was respectively injected i.p.( 3.5mg/kg) on day 2 and day 4 after the insult. The expression of OMP was tested by immunohistochemistry and western blot. Results The expression level of OMP was significantly downregulated in the olfactory mucosa of influenza virus control group 1 and influenza virus control group 2; the expression level of OMP was significantly upregulated in the olfactory mucosa of post-virus Compound Betamethasone group 1 and post-virus Compound Betamethasone group 2. Conclusion Compound Betamethasone can enhance The expression of OMP in the injured olfactory mucosa by influenza virus.
    The measurement of affinity of site-specific labeled annexin V with 99mTc by calcium titration
    Jiong CAI; Fang LI; Na NIU; Shi-zhen WANG
    2010, 30(5):  534-537. 
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    Objective To derermine the affinity of site-specific labeled annexin V with 99mTc to phosphatidylserine (PS) exposed erythrocytes. Methods The annexin V fused with a metal chelating binding site was obtained from pichia pastoris culture and methanol induction expression. The annexin V was purified from the culture supernatant crude product by ultrafiltration. The annexin V was conjugated with 99mTc site-specifically with sodium glucoheptonic acid and SnCl2. The radioactive annexin V was added at wide range of concentration to determine its affinity to PS exposed erythrocytes. Results The calcium concentration at which half of the protein is bound to cells (EC50) is changed with varying ratio of protein to cells. The affinity was determined at lower protein to cells ratio as 33.4. Conclusion The annexin V expressed recombinantly in pichia pastoris show higher affinity to PS exposed erythrocytes.
    Clinical analysis of 8 cases with primary pigmented nodular adrenocortical disease
    Wei LI; Kai FENG; Ou WANG; Quan-zong MAO; Ming-ming HU; Xin YUE; Zhao-lin LU
    2010, 30(5):  538-541. 
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    Objective To summarize the clinical characteristics of primary pigmented nodular adrenocortical disease(PPNAD). Methods 8 patients with PPNAD from 2001 to 2009 in PUMCH were reviewed, their clinical data were collected. Results PPNAD often occurred in adolescents.62.5% of patients with PPNAD were part of Carney complex(CNC).In addition to general features of Cushing's syndrome, amenorrhea and growth retardation in stature were very apparent in clinical manifestations of PPNAD, respectively 100% and 62.5%. Plasma ACTH was undetectable, circadian rhythm of plasma cortisol was disappeared, glucocorticoid excretion was increased paradoxically during the dexamethasone suppression test in 50% patients with PPNAD. Adrenal Imaging in 75% patients revealed normal-sized adrenal glands or suspectable micronodular changes. Adrenal pathologic analysis revealed numerous brown cortical nodules containing lipofuscin pigmentation. Unilateral adrenalectomy may relieve symptoms of Cushing's syndrome, but plasma ACTH and circadian rhythm of plasma cortisol were difficult to be recovered. Hypercorticoidism might recur after unilateral adrenalectomy. Conclusion PPNAD should be bewared in ACTH independent Cushing's syndrome patients without apparent adrenal mass, and CNC should be screened and followed up.
    Insulin reduces ALI induced by LPS in mice
    Li-jing PENG; Bu-xiong TUO; Hui LI; Chao-min LI; Wei LIU; Ming-xia YE
    2010, 30(5):  542-543. 
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    Changes of p53 and Caspase-3 expression at boundary area of Myocardial Ischemia Reperfusion in Rats
    Yan-fang ZHANG; Rui YANG; Fang WANG; Ting-tong YANG
    2010, 30(5):  544-546. 
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    Immune regulatory effect of masenchymal stem cells on T lymphocyte
    Zhi-qiong JIANG; Zhong TANG; Guo-hua YUAN; Jing TAN
    2010, 30(5):  547-549. 
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    Mesenchymal stem cells (MSCs) have a unique role in immune regulation based on T-cells. On the mixed lymphocyte reactions, MSCs have inhibited T-cells proliferation by the effect of cycle arrest, but they do not cause the increase of T-cells apoptosis and the suppression of T-cells activation. In addition, MSCs can reduce CD8+ T cells and Th1 cells, and simultaneously increase Th2 cells in the reaction system to suppress the inflammatory response, which may play a therapeutic effect on the T-cells mediated autoimmune diseases.
    Kazal-type Serine Protease Inhibitors and Human Diseases
    Hai-shen LI; Yun CAO; Chao-nan QIAN
    2010, 30(5):  550-553. 
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    The family of Kazal-type serine protease inhibitor (SPINK) correlates with chronic pancreatitis, Netherton syndrome, esophageal carcinoma, and other humane diseases. The functions of SPINK1, SPINK5 and SPINK7 have been identified. However, other members remain to be explored. In this review, we summarized locations of SPINK genes, protein structures, functions of SPINK proteins, and the relationship between SPINK family and humane diseases.
    Role of autophagy in the pathogenesis of ischemic heart disease
    Gui-lan LI; Hong-shuang YANG; Yan GUO
    2010, 30(5):  554-556. 
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    Autophagy is induced by a diversity of signals during ischemia and reperfusion.Autophagy has been shown to protect cardiac cells and reduce the cell loss, but it also has been shown that enhanced autophagy contributes to cell death during I/R.
    Progress in molecule-targeted therapy for pancreatic cancer
    Sheng LI; Quan LIAO; Yu-pei ZHAO
    2010, 30(5):  557-560. 
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    Pancreatic cancer is one of the most malignant cancer of all neoplasm. 85% cases are not suitable for surgery when first diagnosed. And the effects of chemotherapy and radiotherapy are not satisfactory. Therefore, molecule-targeted therapy becomes a focus in the research of pancreatic cancer recently. EGFR and VGFR inhibition therapy is the emphasis of this field, and show a great prospect in pre-clinical research. And this article reviews progress in the research of molecule-targeted therapy.