全血NPM1、MCP-1和肠道菌群与胃癌进展及预后相关

doi:10.16352/j.issn.1001-6325.2024.08.1137

摘要/Abstract

摘要： 目的探讨全血核仁磷酸蛋白1(NPM1)、单核细胞趋化蛋白-1(MCP-1)、肠道菌群与胃癌进展及预后的相关性。方法选取2018年5月至2020年5月在南阳市第二人民医院收治的胃癌患者120例作为胃癌组,另选取同期胃镜检查胃良性病变患者120例作为胃良性病变组,再选取同期健康体检者120例作为对照组。RT-qPCR检测NPM1 mRNA、MCP-1 mRNA转录水平;用MicroScan微生物鉴定分析系统检测肠道菌群;用Pearson法分析血清NPM1、MCP-1和肠道菌群的相关性;用多因素COX回归分析胃癌患者预后的影响因素。结果对照组、胃良性病变组和胃癌组NPM1 mRNA、双歧杆菌和乳酸杆菌水平依次降低(P＜0.05),而MCP-1 mRNA、肠球菌和大肠埃希菌水平依次升高(P＜0.05)。胃癌Ⅲ~Ⅳ期的NPM1 mRNA、双歧杆菌和乳酸杆菌水平显著低于Ⅰ~Ⅱ期(P＜0.05),MCP-1 mRNA、肠球菌和大肠埃希菌水平显著高于Ⅰ~Ⅱ期(P＜0.05)。Pearson相关性分析表明,胃癌患者NPM1 mRNA与MCP-1 mRNA水平呈负相关(P＜0.05),NPM1 mRNA与双歧杆菌和乳酸杆菌呈正相关(P＜0.05),与肠球菌和大肠埃希菌呈负相关(P＜0.05),MCP-1 mRNA与与双歧杆菌和乳酸杆菌呈负相关(P＜0.05),与肠球菌和大肠埃希菌呈正相关(P＜0.05)。预后不良组NPM1 mRNA、双歧杆菌和乳酸杆菌水平显著低于预后良好组(P＜0.05),MCP-1 mRNA、肠球菌和大肠埃希菌水平显著高于预后良好组(P＜0.05)。COX回归分析表明,MCP-1 mRNA、肠球菌和大肠埃希菌是影响胃癌患者预后的危险因素(P＜0.05),NPM1 mRNA、双歧杆菌、乳酸杆菌是保护因素(P＜0.05)。结论胃癌患者中NPM1、肠道双歧杆菌和乳酸杆菌水平降低,MCP-1、肠球菌和大肠埃希菌水平升高,其均与胃癌进展和预后密切相关。

关键词: 核仁磷酸蛋白1, 单核细胞趋化蛋白-1, 肠道菌群, 胃癌, 预后

Abstract: Objective To investigate the association of whole blood nucleophosmin 1(NPM1), monocyte chemotactic protein-1 (MCP-1), and intestinal flora with gastric cancer progression and prognosis. Methods A total of 120 patients with gastric cancer who were admitted to the Nanyang Second People′s Hospital from May 2018 to May 2020 were selected as the gastric cancer group, 120 patients with benign gastric lesions underwent gastroscopy in the same period were selected as the benign gastric lesions group, and 120 patients with healthy physical examinations in the same period were selected as the control group. RT-qPCR was used to detect mRNA expression of NPM1 and MCP-1. The intestinal microbiota was examined by MicroScan microbial identification analysis system; The correlation between NPM1, MCP-1 and intestinal microbiota was analyzed by Pearson method. Multivariate COX regression was used to analyze the influencing factors of prognosis in gastric cancer patients. Results The level of NPM1 mRNA,exuberance of Bifidobacterium and Lactobacillus in the control group, gastric benign lesion group, and gastric cancer group decreased sequentially (P＜0.05), whereas MCP-1 mRNA, Enterococcus, and Escherichia coli increased sequentially (P＜0.05). Serum NPM1 mRNA, Bifidobacterium and Lactobacillus in stage Ⅲ-Ⅳ of gastric cancer were obviously lower than those found in stage Ⅰ-Ⅱ(P＜0.05), while MCP-1 mRNA, Enterococcus and Escherichia coli were obviously more than those found in stage Ⅰ-Ⅱ(P＜0.05). Pearson correlation analysis showed that, there was a negative correlation between serum NPM1 mRNA and MCP-1 mRNA levels in gastric cancer patients (P＜0.05), NPM1 mRNA was positively correlated with Bifidobacterium and Lactobacillus(P＜0.05) and negatively correlated with Enterococcus and Escherichia coli(P＜0.05). MCP-1 mRNA was negatively correlated with the quantity of Bifidobacterium and Lactobacillus(P＜0.05) but positively correlated with Enterococcus and Escherichia coli(P＜0.05). The level of NPM1 mRNA, quantity of Bifidobacterium and Lactobacillus in the poor prognosis group were obviously lower than those found in the good prognosis group (P＜0.05), while the level of MCP-1 mRNA, quantity of Enterococcus and Escherichia coli were obviously higher than those in the good prognosis group (P＜0.05). COX regression analyses showed that MCP-1 mRNA, Enterococcus and Escherichia coli were risk factors affecting the prognosis of gastric cancer patients (P＜0.05), while NPM1 mRNA, Bifidobacterium, and Lactobacillus were protective factors (P＜0.05). Conclusions Decreased level of NPM1, decreased counting of Bifidobacterium intestinalis and Lactobacillus, increased level of MCP-1, increased counting of Enterococcus and Escherichia coli are strongly associated with gastric cancer progression and poor prognosis.

Key words: nucleophosmin 1, monocyte chemotactic protein-1, intestinal microbiota, gastric cancer, prognosis

中图分类号:

R735.2

侯楠, 刘源, 高俊, 王晶, 袁萌. 全血NPM1、MCP-1和肠道菌群与胃癌进展及预后相关[J]. 基础医学与临床, 2024, 44(8): 1137-1142.

HOU Nan, LIU Yuan, GAO Jun, WANG Jing, YUAN Meng. Whole blood NPM1, MCP-1 and intestinal flora are associated with gastric cancer progression and prognosis[J]. Basic & Clinical Medicine, 2024, 44(8): 1137-1142.

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