ASH2L在幽门螺旋杆菌感染的人胃癌细胞系中的表达及临床意义

doi:10.16352/j.issn.1001-6325.2023.03.367

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (3): 367-373.doi: 10.16352/j.issn.1001-6325.2023.03.367

• 研究论文 • 下一篇

ASH2L在幽门螺旋杆菌感染的人胃癌细胞系中的表达及临床意义

谢晓婷¹, 张雷^1*, 张莉^1*, 董凤¹, 刘颖^1,2, 李光华¹, 杨兴¹

1.大理大学病原与媒介生物研究所云南省自然疫源性疾病防控技术重点实验室, 云南大理 671000;
2.吉林大学第一医院二部检验科,吉林长春 130000

收稿日期:2022-04-22 修回日期:2022-10-03 出版日期:2023-03-05 发布日期:2023-02-27
通讯作者: * leigezhang@163.com;nizi9358@163.com
基金资助:
国家自然科学基金(81560096);大理大学临床分子免疫学创新团队项目(ZKLX2019105)

Expression and clinical significance of ASH2L in human gastric carcinoma cell lines infected with Helicobacter pylori

XIE Xiaoting¹, ZHANG Lei^1*, ZHANG Li^1*, DONG Feng¹, LIU Ying^1,2, LI Guanghua¹, YANG Xing¹

1. Institute of Pathogens and Vectors, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali University, Dali 671000;
2. Department of Clinical Laboratory, the Eastern Division, First Hospitipal of Jilin University, Changchun 130000,China

Received:2022-04-22 Revised:2022-10-03 Online:2023-03-05 Published:2023-02-27
Contact: * leigezhang@163.com;nizi9358@163.com

摘要/Abstract

摘要： 目的探索果蝇蛋白Ash2的类似物(ASH2L)在幽门螺旋杆菌(Hp)感染的人胃癌细胞系中的表达水平及临床意义。方法利用TIMER2.0、UALCAN等生物信息软件分析ASH2L在多种肿瘤中的表达水平、与胃腺癌患者临床病理特征及与预后的关系。用Hp分别感染人胃上皮细胞系GES、人胃腺癌细胞系SGC-7901和AGS,以RT-qPCR、Western blot检测ASH2L在Hp感染前后的表达水平。结果生物信息分析显示ASH2L在15种肿瘤中存在差异表达,且其高表达于胃腺癌组织中;在胃癌患者中,ASH2L高表达者总生存期(OS)和后进展生存期(PPS)低于低表达者(P＜0.05);不同年龄组、不同种族、不同病理分级的胃腺癌患者中ASH2L的表达存在一定的差异,但与男女性别无关;Hp感染的3种人胃上皮细胞系中ASH2L表达水平均显著上调(P＜0.05)。结论 ASH2L在Hp感染引发的胃癌的发生发展中可能发挥重要作用,其表达水平与胃癌患者各种临床病理特征及预后密切相关,可望作为判断预后的分子标志物。

关键词: 幽门螺旋杆菌, 胃癌, ASH2L, 临床预后

Abstract: Objective To explore the expression and clinical significance of Drosophila protein Ash2 analogue(ASH2L)in gastric carcinoma cell lines infected with Helicobacter pylori(Hp). Methods The expression of ASH2L in various tumors, the relationship between ASH2L and pathological characteristics of stomach adenocarcinoma and the prognosis of the patients were analyzed by TIMER2.0, UALCAN and other software. Human gastric mucosal epithelial cell line GES, gastric carcinoma cell lines SGC-7901 and AGS were infected with Hp and the expression of ASH2L before and after Hp infection was detected by RT-qPCR and Western blot. Results The analysis showed that ASH2L was differentially expressed in 15 kinds of tumors and was highly expressed in stomach adenocarcinoma. The overall survival (OS) and post-progression survival (PPS) of patients with high ASH2L expression were lower than those with low ASH2L expression(P＜0.05). There were some differences in the expression of ASH2L in patients with stomach adenocarcinoma in different age groups, different races and different pathological grades, but not related to gender. The expression of ASH2L was significantly up-regulated in three human gastric epithelial cell lines infected with Hp(P＜0.05). Conclusions ASH2L may play an important role in tumorigenesis of gastric carcinoma caused by Hp infection, and its expression level is closely related to various pathological features and prognosis of the patients, and it is expected to be a molecular marker for prognosis.

Key words: Helicobacter pylori, gastric carcinoma, ASH2L, clinical prognosis

中图分类号:

R378

谢晓婷, 张雷, 张莉, 董凤, 刘颖, 李光华, 杨兴. ASH2L在幽门螺旋杆菌感染的人胃癌细胞系中的表达及临床意义[J]. 基础医学与临床, 2023, 43(3): 367-373.

XIE Xiaoting, ZHANG Lei, ZHANG Li, DONG Feng, LIU Ying, LI Guanghua, YANG Xing. Expression and clinical significance of ASH2L in human gastric carcinoma cell lines infected with Helicobacter pylori[J]. Basic & Clinical Medicine, 2023, 43(3): 367-373.

参考文献

[1]Sung H, Ferlay J, Siegel R L, et al. Global Cancer Statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021,71:209-249.
[2]Lamb A, Chen L F. Role of the Helicobacter pylori-induced inflammatory response in the development of gastric cancer[J]. J Cell Biochem, 2013,114:491-497.
[3]Ren S, Cai P, Liu Y, et al. Prevalence of Helicobacter pylori infection in China: a systematic review and meta-analysis[J]. J Gastroenterol Hepatol, 2022,37:464-470.
[4]Cho J, Prashar A, Jones N L, et al. Helicobacter pylori infection[J]. Gastroenterol Clin North Am, 2021,50:261-282.
[5]van Den Brink GR, Ten KF, Ponsioen CY, et al. Expression and activation of NF-kappa B in the antrum of the human stomach[J]. J Immunol, 2000,164:3353-3359.
[6]Ruthenburg AJ, Allis CD, Wysocka J. Methylation of lysine 4 on histone H3: intricacy of writing and reading a single epigenetic mark[J]. Mol Cell, 2007,25:15-30.
[7]Luscher-Firzlaff J, Gawlista I, Vervoorts J, et al. The human trithorax protein hASH2 functions as an oncoprotein[J]. Cancer Res, 2008,68:749-758.
[8]Li T, Fan J, Wang B, et al. TIMER: a Web server for comprehensive analysis of tumor-infiltrating immune cells[J]. Cancer Res, 2017,77:e108-e110.
[9]Chandrashekar DS, Bashel B, Balasubramanya S, et al. UALCAN: a portal for facilitating tumor subgroup gene expression and survival analyses[J]. Neoplasia, 2017,19:649-658.
[10]Yang Z, Xie C, Xu W, et al. Phosphorylation and inactivation of PTEN at residues Ser380/Thr382/383 induced by Helicobacter pylori promotes gastric epithelial cell survival through PI3K/Akt pathway[J]. Oncotarget, 2015,6:31916-31926.
[11]Johnston FM, Beckman M. Updates on management of gastric cancer[J]. Curr Oncol Rep, 2019,21:67.doi:10.1007/s11912-019-0820-4.
[12]Zeng K, Wu Y, Wang C, et al. ASH2L is involved in promotion of endometrial cancer progression via upregula-tion of PAX2 transcription[J]. Cancer Sci, 2020,111:2062-2077.
[13]Constantin D, Widmann C. ASH2L drives proliferation and sensitivity to bleomycin and other genotoxins in Hodgkin's lymphoma and testicular cancer cells[J]. Cell Death Dis, 2020,11:1019.doi:10.1038/s41419-020-03231-0.
[14]Butler JS, Qiu YH, Zhang N, et al. Low expression of ASH2L protein correlates with a favorable outcome in acute myeloid leukemia[J]. Leuk Lymphoma, 2017,58:1207-1218.
[15]Dou Y, Milne TA, Ruthenburg AJ, et al. Regulation of MLL1 H3K4 methyltransferase activity by its core components[J]. Nat Struct Mol Biol, 2006,13:713-719.

ASH2L在幽门螺旋杆菌感染的人胃癌细胞系中的表达及临床意义

Expression and clinical significance of ASH2L in human gastric carcinoma cell lines infected with Helicobacter pylori

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