Table of Content

05 September 2011, Volume 31 Issue 9

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Effects of curcumin on heart development-related genes expression and the associated epigenetic mechanism

2011, 31(9):  959-964. 

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Objective To investigate the effects of curcumin on heart development-related genes GATA4, MEF2C and Nkx2.5, and to explore the epigenetic regulation mechanism. Methods Neonatal mouse cardiocytes were treated with curcumin with different concentration and different time. ELISA was used to detect the histone acetylase (HATs) activity of cardiocytes, western blot to detect the acetylation of histone H3, real time RT-PCT to measure the expression of heart development-related genes GATA4, MEF2C and Nkx2.5, and chromatin immunoprecipitation (ChIP)-PCR to analyse the chromatin struction. Results 30mol/L of curcumin could inhibit the HAT activity and the acetylation of histone H3 at 24 hours. Accordingly the expression of GATA4, MEF2C and Nkx2.5 were repressed significantly (P<0.05). The result of ChIP-PCR showed that the the acetylation of histone which bound with promoter regions of these genes were reduced. Conclusion Curcumin could inhibit the HAT activity and repress the expression of heart development-related genes GATA4, MEF2C and Nkx2.5 effectively. And the loose structure of chromatin induced by hypoacetylation of histone bound with promoter regions might be one mechanism of this effect.

HSP47 participating in the expression of COLLAGEN in human embryonic lung fibroblast induced by TGF－β1

2011, 31(9):  965-969. 

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Abstract:Objective By investigating effect of the dynamic expression of HSP47 in human embryonic lung fibroblast (HELF) on pulmonary fibrosis to explore the relation between HSP47 and pulmonary fibrosis . Methods Dynamic expression of HSP47、COLLAGENⅠ、α-SMA and VIMENTIN were observed after HSP47 was induced to expressed in HELF in incubation of TGF-β1. HELF were stimulated by TGF-β1(5ng/mL) at different time points and different dosages for 48h respectively. The proliferative rate of HELF was tested by the method ofWST.Expression of HSP47 was determined by immunofluorescence.Expressions of HSP47、COLLAGEⅠ、α-SMA and VIMENTIN were detected by Western blot. Results HSP47 showed slight expression in normal HELF. Expressions of HSP47, COLLAGENⅠ,α-SMA and VIMENTIN increased synchronously by means of time-independence and dosage-independence after HELF being treated by TGF-β1（P<0.05）.Conclusions HSP47 participated in the expression of collagen in human embryonic lung fibroblast induced by TGF－β1 that suggested HSP47 might play an important role in pulmonary fibrosis as collagen-specific molecular chaperone.

Possible protective effects of secretory phospholipase A2 inhibitor from snake serum against human inflammation: based on protein structure and function

2011, 31(9):  970-975. 

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Abstract: Objective To compare the structural similarities of human secretory phospholipase A2 (sPLA2) with snake venom sPLA2, and to analyze the neutralization effect of sPLA2 inhibitors (PLIs) from snake serum against sPLA2 by exploring their structure and function relationship, which was meaningful for assessing PLIs’ clinical application in inflammation protection. Methods Multiple sequencial alignment of 7 human sPLA2 and 3 five-pace snake venom sPLA2 was made by Clustal W. Tertiary structures of the 10 sPLA2 were predicted by Swiss-Modelling. Spatial structure similarity between snake venom and human sPLA2 was assessed by UCSF Chiema. Results Human and snake venom sPLA2 shared 40% sequencial homologous and approximately similar spatial structure. All the PLIs (γ type) have similar tertiary charectristics and conserved binding region是 to sPLA2. Conclusion Based on the bioinformatical analysis, snake serum PLIs were considered as an effective inhibitor to snake venom toxicity and human sPLA2 activity. Thus, the PLIs were potential special blockers to human sPLA2 inflammation pathway and meaningful for curing clinical disease.

Investigation of inhibition of adenovirus mediated gene ING4 and PTEN to hepatocarcinoma Cells

2011, 31(9):  976-979. 

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[Abstract] Purpose: We use recombinant Ad-ING4 and Ad-PTEN to infect human hepatocarcinoma cell line SMMC-7721 in vitro, test and compare the effects of gene expression of the cells. Method: We use recombinant Ad-ING4 and Ad-PTEN and control virus to infect QBI-293A cells for viral propagation and viral titer detection. We confirm the infective effects of Ad-ING4, Ad-PTEN and control virus by fluorescence observation. Use Ad-ING4 and Ad-PTEN to infect SMMC-7721 cells and confirm their effects by their apoptotic rate and the results of MTT. Results: The apoptotic rate of control virus group is 3.4±1.3%, the Ad-ING4 group is 49.7±4.8%（p＜0.01）and the Ad-PTEN group is 11.4±3.2%（p＜0.01）. The cell proliferation of group Ad-ING4 and Ad-PTEN obviously decreased from 48h to 96h（p＜0.05）.Conclusion: The data analysis showed Ad-ING4 and Ad-PTEN had an inhibitory effect to SMMC-7721 cells, and the effect of Ad-ING4 is more severe.

Overexpression of HSP72 in cortex and hippocampus after experimental subarachnoid hemorrhage in rats

2011, 31(9):  980-983. 

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Objective The 72-kDa heat shock protein (HSP72) is a highly stress-inducible member of a chaperone family of proteins. Overexpressing HSP72 is protective against focal and global cerebral ischemia. We assessed the expression of HSP72 in cortex and hippocampus after experimental subarachnoid hemorrhage in rats. Methods 36 Wistar rats were randomly divided into three groups: single intracisternal blood injection, double intracisternal blood injection and sham operation. The rat in intracisternal blood injection groups were subjected to injection of autologous blood into cisterna magna once or twice on day 0 and day 2 and were sacrificed on days 3, respectively. The expression of HSP72 in hippocampus and cerebral cortex was detected by immunohistochemistry and Western-blot technique. Results HSP72 expression was detected in intracisternal blood injection group by immunohistochemistry. Semiquantitative analysis of HSP72 electrophoretic bands indicated more expression in hippocampus and cerebral cortex in both injection groups compared with the sham group(P＜0.01). While there was no significant differences in the two injection groups(P＞0.05). Conclusion Subarachnoid hemorrhage produced by single and double intracisternal autogenious blood injection can cause HSP72 overexpression in hippocampus and cerebral cortex.

Effect on APP expression by up-regulation of Histone Acetylation through p38MAPK pathway

2011, 31(9):  984-987. 

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ABSTRACT Objective To investigate the role of p38MAPK in the expression of β-Amyloid precursor protein (APP) and it’s epigenetic mechanisms. Methods Cultured SH-SY5Y cells were used to test the expression of APP, histone acetyltranferase (HAT) and histone deacetyltranferase (HDAC) by Western blot after activation of p38MAPK; Acetylation level of histone H3 andH4 were examined by optical density assay. Results APP expression was increased after p38MAPK activation for 72 hours compared to the control. Levels of histone H3 acetylation were increased accompanied with up-regulation of CBP and down-regulation of HDAC3. Conclusion APP Expression could be increased by up-regulation of Histone Acetylation through p38MAPK Pathway.

Sunitinb Inhibits the proliferation of human bladder cancer line T24 in vitro

2011, 31(9):  988-990. 

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Abstract purpose : The study is aimed at determining inhibition effect on human bladder cancer line T24 by sunitinb in vitro . Methods : T24 bladder cells line were treated by 0.625、1.25、2.5、5、10 and 20μmol/L sunibinib in 24、48、72 h. The cytotoxic activity was analyzed by MTT . Results Sunitinib inhibited T24 cell proliferation in dose-dependent manner. When the plasma drug concentration exceeded up to 5μmol/L , the inhibitory rate of Sunibinib was more than Cisplatin （P<0.05）. Inhibitory rate of Sunibinib increased with the extension of incubation time , which showed no significant difference to Cisplatin（P>0.05） . Conclusions : Sunitinib inhibited bladder cell line T24 proliferation in dose-dependent and time dependent manner .

Study on association of mdr1 gene tagSNPs rs3789243 and rs2235046 polymorphism with intractable epilepsy

2011, 31(9):  991-995. 

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Objective To investigate the relationship between polymorphisms of MDR1 gene tagSNPs and intractable epilepsy in Han Chinese population．Methods 149 Han epilepsy and 88 Han healthy controls living in or near Anhui Hefei were recruited in this study．Tagging SNPs rs3789243 and rs2235046 were genotyped through PCR-CTPP and the same polymorphism was genotyped by DNA sequence analysis , assaying in all subjects．Results There was significant difference of genotypic CC of rs3789243 between epilepsy group and the controls, intractable epilepsy group and the controls, intractable epilepsy group and seizure-free group (P<0.01),with OR of 6.135、1.792 and 1.254 (all P <0.01); There was significant difference of genotypic AA of rs2235046 between epilepsy group and the controls (P<0.01),with an OR of 2.976(P<0.01). Conclusion Plymorphisms of MDR1 gene may associate with epilepsy:the genotype rs3789243 CC and rs2235046 AA may associate with higher risk for epilepsy; the genotype rs3789243 CC may associate with higher risk for intractable epilepsy.

Dystrophic epidermolysis bullosa: Two families investigation and detection of the mutation of COL7A1 gene

2011, 31(9):  996-999. 

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Two families investigation on dominant dystrophic epidermolysis bullosa and detection of the mutation of COL7A1 gene are reported．The proband of the first family was a 6-year-old girl，who presented with a 6-year history of pruritc vesicles and papules on both shins. the reduced numbers of anchoring fibrils and basal keratinocyte vacuolization were found below the lamina densa of the basement membrane zone. Out of 26 members of 4 generations in the pedigree only the first proband, the others were normal, his parents were consanguineous marriage. diagnosed as recessive dystrophic epidermolysis bullosa (RDEB), generalized other. The proband of the second family was a 6-year-old-boy，who presented with pruritc vesicles and anabrosis on both shins. absent or rudimentary anchoring fibrils were also found below the lamina densa of the basement membrane zone. Out of 20 members of 3 generations in the pedigree only the second proband, the others were normal. diagnosed as RDEB, severe generalized. The COL7A1 gene mutation have not dicovered as others’reported in Chinese patients.

Inhibitory effect of filamin A on invasion and metastasis of human colon carcinoma cell line SW480 in vitro

2011, 31(9):  1000-1005. 

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Abstract:Objective To explore the inhibitory effect of Filamin A(FLNa) on invasion and metastasis of human colon carcinoma cell line SW480 and investigate its mechanisms of the action.Methods Expressing vector pcDNA3.1/V5-His-TOPO/FLNa was transfected into SW480 cells by lipofectamine protocols.After G418 selection,the cells SW480/FLNa expressing FLNa stably were obtained.Expression of FLNa in tumor cells was confirmed by reverse transcription polymerase chain reaction(RT-PCR) and Western blot.RT-PCR assay and Western blot was also employed to detect the expression of matrix metalloproteinases-9（MMP-9）.Cell proliferation in vitro were assessed by MTT assay.Inhibitory effect of filamin A on SW480 cells were observed by Wound healing assay,Transwell chamber experiment,and Matrigel invasion assay.Results The results of RT-PCR and Western blot demonstrated that the recombinant vector was correctly transfected into SW480 cells by liposome.The expression of MMP-9 mRNA and protein was lower in the SW480/FLNa cells than that in the wild SW480 cells；The inhibitory effect of FLNa on invasion and metastasis of SW480 cells were proved by wound healing assay,Transwell chamber experiment and matrigel invasion assay.Colusion FLNa has significant inhibitory effect on the invasion and metastasis of human colon carcinoma cell line SW480.The mechanism of action may be ralated with the suppressive effect of MMP-9.

E-cadherin siRNA promotes drug resistance formation and proliferation in gastric cancer SGC7901 cell linein gastric cancer SGC7901 cell line

2011, 31(9):  1006-1010. 

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Objective: To explore the effect of E-cadherin siRNA on drug sensitivity and cell proliferation and their related mechanisms in SGC7901 cell line. Methods: SGC7901 cell line was transfected with E-cadherin siRNA by means of Lipofectamine 2000. Drug sensitivity of transfected cells to CDDP、5-FU、Paclitaxel and ADR was tested using MTT assay. And proliferation changes of cells were detected after transfection. Protein changes of E-cadherin, PTEN, AKT, p-AKT, Bax and Bcl-2 were detected by Western blot. Results: The E-cadherin protein expression was significantly decreased after E-cadherin siRNA transfection(P<0.05). The IC50 of CDDP、5-FU、Paclitaxel and ADR in E-cadherin siRNA tansfected group were significantly higher than in negative control group(4.375±0.199mg/L vs 2.882±0.166mg/L, 6.855±0.780mg/L vs 4.058±0.946mg/L, 2.530±0.259mg/L vs 1.483±0.225mg/L, 0.368±0.042mg/L vs 0.228±0.012mg/L respectively, P<0.05). Compared to the control group, cell proliferation was significantly increased in SGC7901 cell transfected with E-cadherin siRNA. The expression of PTEN and Bax protein were significantly lower than the control group 72h after trasfection(P<0.05), while the p-AKT and Bcl-2 protin was higher than the control group (P<0.05). Conclusion: E-cadherin siRNA can activate AKT pathway and regulate apoptotic balance, which may be one of mechanisms on promoting drug resistance and proliferation.

The expression of VEGF and endoglin of synovial tissues in Rat induced by Type Ⅱcollagen

2011, 31(9):  1011-1014. 

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【Abstract】Objectives: To investigate the expression and significance of VEGF and endoglin of synovial tissues in rats induced by Ⅱcollagen. Methods: The experimental arthritis model was established by subcutaneous injection ofⅡcollagen. Morphological changes were found through observing the secondary paw swelling and histological morphological of synovial tissues in rats. The expression of VEGF and endoglin protein was detected through immunohistochemistry and RT-PCR techniques. Results: Most arthritis take place in 2~3 weeks after collagen injection. The sympotom includes such as: swelling of the paws and limited movement of joint. The expression of VEGF and endoglin was higher in the synovium of CIA than that of normal rats（p<0.05）. There was a strong positive relationship between VEGF and endoglin,( p<0.05）.Conclusions: They were involved in the pathogenesis of RA. VEGF and endoglin play impotent roles in RA response and in the cytokine web of new synovium vascularization, VEGF and endoglin affect and promote those of each other , and may regulate the vicious web circulation. They were the keys of many cytokines that mediate RA pathogenesis, development, invading, and joint deformation.

Effect of ulinastatin on mRNA of cytokine and p38 MAPK expression of rats with acute lung injury

2011, 31(9):  1015-1020. 

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Objective To explore the protective effect of ulinastatin (UTI) in a rat model of lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the underlying molecular mechanism. Methods Wistar rats were randomly assigned into: control group, model group (LPS 5mg/kg, iv), and intervention group (UTI 50000U/kg, iv). Expression of TNF-alpha and IL-10 mRNA in lung tissue were measured by real time RT-PCR. Expression of phosphorylated p38 MAPK in lung tissue were detected by immunohistochemical staining and Western blot methods. Results Expression of TNF-alpha mRNA at 0.5,1 and 3h in rats of model group is 78.55±18.99,128.74±34.79和12.29±1.32 folds against control group, which decreased to 20.95±1.45（p<0.01）,58.15±11.01（p<0.01）和2.85±0.57（p<0.05）folds in intervention group. Expression of IL-10 mRNA at 0.5,1 and 3h in rats of model group is 20.89±4.60,38.20±8.26和53.26±8.01 folds against control group, which increased to 66.77±11.18（p<0.05）,97.69±27.00（p<0.01）和128.62±42.30（p<0.01）folds against invention group. Administration of LPS elevated the expression of p38 MAPK, which were significantly attenuated by UTI at each time point((p<0.05). Conclusion UTI could attenuate ALI by regulating the gene expression of cytokines. P38 MAPK played role in the decreasing of TNF-alpha mRNA by UTI.

Effects of 1,25-(OH)2D3 and dexamethasone on airway remodeling in model murine of chronic asthma

2011, 31(9):  1021-1025. 

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Objective To investigate the effects of 1,25-(OH)2D3 on airway remodeling and the expression of matrix metalloprotease-9 (MMP-9) in a murine model of chronic asthma, and to explore the potential mechanism of 1,25-(OH)2D3 in the treatment of asthma.Methods BALB/c mice were sensitized and challenged with ovalbumin to establish the chronic asthmatic model.They were randomly divided into four groups: control group, asthma group, dexamethasone (DEX) group and VD group.The characteristic airway inflammation and alteration of airway structure were detected by HE staining. Morphometric analysis of the stained sections was performed using computerized image analysis system.The expression of MMP-9 in both activity and mRNA level was detected by gelatin zymograph and RT-PCR, respectively.Results (1)The infiltration of inflammatory cells, epithelial loss, smooth muscle cell layer thickening were decreased in DEX group and VD group when compared with those in asthma group(P<0.05), but these changes were both more significant than those in the control group(P<0.05); (2)WAm /Pbm、WAi/Pbm and N/Pbm decreased significantly in VD group when compared with those in asthma group(P<0.05), but they were still higher than those in the control group (P<0.05); (3)The activity and mRNA level of MMP-9 in VD group was decreased when compared with those in asthma group(P<0.05), but it was still higher than those in the control group(P<0.05). Conclusions Intervention with 1,25-(OH)2D3 and dexamethasone could markly alleviate the asthmatic airway remodeling and partly restore of appropriate structure of airway wall.It could also lower the expression of MMP-9 in the lung tissue on asthmatic condition,thus delay the process of airway remodeling.

Bone marrow mesenchymal stem cells transplantation for the reduction of intestine injury in aging rat

2011, 31(9):  1026-1031. 

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Objective To observe the effect of bone-marrow mesenchymal stem cells on intestine in the aging rats induced by D-galactose. Methods Healthy SD rats were divided into three groups at random: normal control group, aging model group and MSCs group. The aging model group were injected with D-galactose for 4 months to establish the aging rats. The contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in intestine were measured wtih hydroxylamine and chromatometry respectively. The pathological morphological changes of intestine were observed. MSCs were transfected by lentiviral vectors expressing green fluorescent protein (GFP) to construct GFP-MSCs, and the migration of MSCs in intestine was examined. Results MSCs marked by GFP could migrate into the intestine. Compared with the aging model group，the activity of SOD was significantly lower，the content of MDA increased significantly in MSCs group, and there were significant differences:（133.7±3.6）, （105.1±4.3）U/ml, P <0.01; （5.9±0.1）, （6.9±0.1）(nmol/ml), P <0.01. The intestinal mucosa injury is serious in aging model group rats; and the intestine pathological injury had obvious repaired in MSCs group, compared with aging model group. Conclusion These results support the idea that transplantation of mesenchymal stem showed a protective effect on intestine damage induced by D-galactose.

The effects of aldosterone on the expression of PTEN in cultured neonatal cardiac fibroblasts

2011, 31(9):  1032-1035. 

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Analysis, characterization and purification of allergen in Bischofia polycarpa pollen

2011, 31(9):  1036-1039. 

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【Abstract】 Objective To characterize and purify the allergenic components of Bischofia polycarpa pollen. Methods Pollen extract from Bischofia polycarpa pollen prepared and analyzed by SDS-PAGE and proteins band were visualized by staining the gel with Coomassis blue. Antigenic properties of the separated proteins were analyzed by immuno-blotting. Ion-exchange chromatography with DE-52 was used for the purification of the antigens. Then purified products were characterized by western-blotting. Results For the pollen extract of Bischofia polycarpa, 18 protein bands was in SDS-PAGE. Two of these protein bands, with a molecular weight of 12 000 Mr ang 14 000 Mr, showed immunoreactivity with IgE in the sera from patients with pollen allergy of Bischofia polycarpa. Result from the ion-exchange chromatography indicated that the two pollen allergens (12 000 、14 000 Mr) from Bischofia polycarpa were eluted in the second peak. Conclusion Pollen allergens form Roystonea regia were purified and characterized.The result will provide a basis for the diagnosis and treatment of allergy to the pollen allergens form Bischofia polycarpa

The CT manifestations of ovarian fibroma and fibrothecoma

2011, 31(9):  1043-1046. 

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Objective To analyse the CT manifestations of ovarian fibromas and fibrothecomas, and to improve the accuracy rate of the diagnosis. Methods The clinical and CT characteristics of the 12 cases with pathologically confirmed ovarian fibroma or fibrothecoma were reviewed. Results (1)Of the 12 cases, 5 were fibromas and 7 were fibrothecomas. (2)All the cases were unilateral, 5 were in the right and 7 were in the left. (3)On CT imaging, fibromas were solid and with shallow leaflet in big tumors. Fibrothecomas were solid or cystic-solid; the cystic part of every tumor was single, and the cystic walls were commonly uniform thickness and were extension of the solid part. All the tumors had clear borders; and had not obvious effect in contrast enhancement. Conclusion It is affirmative characteristics of ovarian fibromas and fibrothecomas on CT imaging.

Quantification of long chain acyl-CoA in muscles in high-fat fed mice and high-fructose fed mice and its association with insulin resistance

2011, 31(9):  1047-1048. 

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Abstract: Objective To quantify the contents of different species of long chain acyl-CoA( LCACoA ) in muscle tissue in both high-fat fed mice and high-fructose fed mice. To study the association of LCACoAs with insulin resistance through two different diet-induced insulin resistant animal models. Methods Male Wistar rats (~300g) were fed a standard chow (Con), Fru (~34.5%) or Fat (~60%) diet for 3 weeks. Fasting blood glucose, plasma insulin, HOMA index, plasma triglyceride, triglyceride content and LCACoAs in muscle tissues were measured. Results Compared with control group, blood glucose, plasma insulin, HOMA-IR index, plasma triglyceride, triglyceride contents and total LCACoA content in muscle tissues were significantly increased in both high-fat group and high-fructose group. Total LCACoA contents in muscles were positively correlated with HOMA index. Conclusion Whole-body insulin resistance and LCACoAs aumulation can be induced by both high-fat feeding and high-fructose feeding. LCACoA content in muscle is associated with the development of insulin resistance.

Effect of Protoporphyrin Disodium on SOD and MDA in Liver Tissue of Mice with Acute Hepatic Injury

2011, 31(9):  1049-1050. 

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Objective To study the effect of protoporphyrin disodium (NAPP) on activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in liver tissue of mice with acute hepatic injury induced by carbon tetrachloride (CCl4). Methods 60 ICR mice were randomly divided into normal control group, CCl4 model group, bifendate pills group, NAPP low dose group, NAPP medium dose group and NAPP high dose group. Mice of each treatment group were injected different drugs by intragastric administration continuously and then a single dose of CCl4 was given to establish acute hepatic injury model. 16 hours later, blood was collected by picking eyeballs to detect the activities of ALT and AST in serum, liver was taken out to detect the activity of SOD and the content of MDA in liver tissiue of each group. Results Compared with the normal control group, the activities of ALT and AST in serum of the CCl4 model group were (1879±1219) U/L and (2210±1585) U/L, there has a significant difference (P<0.01); The activities of SOD and MDA in the liver tissues of the bifendate pills group, NAPP low dose group, NAPP medium dose group and NAPP high dose group were (207.61±16.02), (184.35±13.42), (190.88±17.77), (199.38±14.43) U/mgprot and (1.08±0.15), (1.35±0.26), (1.07±0.16), (0.92±0.18) nmol/mgprot, respectively, which have statistical sense compared with model group (P<0.05～P<0.01). Conclution NAPP can effectively prevent the decrease of SOD and increase of MDA in liver tissues of mice with acute hepatic injury, this shows NAPP has a good effect of protecting liver and decreasing enzymes on mice with acute hepatic injury induced by CCl4.

The effect of adrenomedullin in oleic acid-induced acute lung injury in rats

2011, 31(9):  1051-1052. 

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Effects of Sirolimus-eluting stents implantation on the expression of the TNF-alpha

2011, 31(9):  1053-1054. 

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Objective: The purpose of this study was to determine the effects of Sirolimus-eluting stents on the expression of the TNF-alpha and the mechanism of Sirolimus-eluting stents implantation for prevention of in-stent restenosis. Methods: Fourteen male miniature porcine were randomized (seven animals per group) to receive either bare-metal stents or sirolimus-eluting stents. The stented-coronary arteries were harvested at 4 weeks after stent implantation and Serial peripheral blood samples were obtained before stent implantation , at 1 and 4 weeks after the implantation. Reverse transcription polymerase chain reaction (RT-PCR) method was taken to detect the expression of TNF-alpha mRNA. The level of tumor necrosis factor-alpha (TNF-alpha) in serum were assessed by enzyme-linked immunosorbent assay (ELISA). Results: TNF-alpha serum levels in Sirolimus-eluting stents group did not differ before stent implantatio，at 1 and 4 weeks after the implantation. In bare-metal stents group, TNF-alpha serum levels increased gradually, especially TNF-alpha serum levels at 4 weeks after stent implantation enhanced significantly compared with before stent implantation. TNF-alpha serum levels before stent implantation and at 1 weeks did not differ between both groups, whereas TNF-alpha serum levels at 4 weeks after stent implantation is significantly higher in bare-metal stents group. The RT-PCR analysis revealed an reduced expression of TNF-alpha mRNA in the Sirolimus-eluting stents group than in the bare-metal stents group. Conclusions: Sirolimus-eluting stents can inhibit the expression of the TNF-alpha mRNA and strut-associated inflammatory response in the porcine coronary model.

Expression changes of large conductance Ca2+-activated K+ channel on rats aortas during hypertension

2011, 31(9):  1055-1056. 

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Objective to study the expression changes and mechanism of large conductance Ca2+-activated K+ channel on aortas between SHR and WKY. Methods the expression of BKα subunit andβ1 subunit on aortas between SHR and WKY by means of real-time quantitative PCR and western blotting.Results In mRNA study,expression ofαandβ1 subunit are higher in SHR than WKY(n=6,p<0.05).In protein expression,αsubunit has no significant difference between SHR and WKY(n=6,p>0.05),butβ1subunit in SHR is more than WKY, and there was significant difference(n=6,p<0.05).Conclusions the expression of BKα subunit andβ1 subunit mRNA and protein in SHR are higher than that in WKY which may be a Negative feedback mechanism to Vascular tone during hypertension.

Research progress of low-density lipoprotein receptor-related protein 5 and bone mass

2011, 31(9):  1060-1063. 

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Abstract：Bone mineral density(BMD), as an clinical marker for diagnosis of osteoporosis and assessment of fracture risk, is likely affected by multiple genetic and environmental factors. Recently, researches suggest that low-density lipoprotein receptor-related protein 5(LRP5) is closely related to bone mass and differentiation of osteoblasts. Furthermore, LRP5 is also associated to BMD and/or osteoporotic fracture. We review LRP5 gene locus, expression，structure，function and association between LRP5 and bone mass, in order to investigate the likely mechanisms of the association.

Low Molecular Weight Cyclin E and its Relation with Malignant Tumors

2011, 31(9):  1064-1066. 

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Cyclin E/cyclin-dependent kinase2 (CDK2) complex plays a key role in the G1/S transition of cell cycle. Full-length cyclin E (FL-E) is mainly expressed in normal tissues and cells, whereas low molecular weight cyclin E (LMW-E) can only be found in tumors. LMW-E is more stable with higher cyclin E/CDK2 kinase activity compared to FL-E, results in genomic instability and deregulation of cell cycle, and leads to endocrine resistance in breast cancer treatment. As an important biological marker of tumor prognosis, LMW-E may also be a potential target for its biological treatment.

THE RESEARCH FOR THE CORRELATION OF PUMA TO TUMOR

2011, 31(9):  1067-1069. 

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Abstract Puma(p53 up-regulated modulator of apoptosis), a pro-apoptotic member of the Bcl-2 family, has a potential pro-apoptotic activity. Some studies have shown that Puma may be associated with the inhibiting of tumor and induce tumor apoptosis by many ways. In the therapy for tumor, the up-regulated Puma is benefit for chemotherapy, the deletion of Puma is benefit for relieving the side effect of radiotherapy. Recently, the correlative reasearch about the apoptosis of the tumor and the therapy with Puma had became hot, This article will discuss the correlative reasearches for Puma to tumor.

The role of NF-κ B in some cardiovascular diseases.

2011, 31(9):  1070-1073. 

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Cardiovascular diseases are still the primary cause of death worldwide. The molecular mechanisms have not been fully elucidated, however the nuclear factor-kappa B seems to link with cardiovascular diseases. In recent years, the importance of NF-κ B in cardiovascular disease has received much attention. In this paper, the following contents were reviewed, which included the physiological characteristic of NF-κ B, the role of NF-κ B in hypoxia inflammation, the role of NF-κ B in endothelial cell apoptosis, the relationship between NF-κ B and some cardiovascular diseases pathology.

Thinking of improving the retrieval language and the information literally education

2011, 31(9):  1074-1076. 

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With the development of internet and information technology, the curriculum of information retrieval language has increased the content of information literary in Peking Union Medical College. We teach the students how to use the tool of clinical information organization which means the concept has more widely than past. Now our education focus on the relationship of retrieval language and searching information, retrieval language and information organization as well as the information organization and personal development. Use 5W2H(what、why、where、when、who、how、how much) analysis, comparison of the common characteristics of medical interrogation language and information retrieval language. In the class, we required the students to take group discussions and representative reporting to share their ideas and to provoke learning interest. We required the students to take group discussions and representative reporting to share their ideas which could intrigue their interesting. Now, it has proved that it help the students a lot when they searching and organizing information.