Abstract: Abstract: Objective To quantify the contents of different species of long chain acyl-CoA( LCACoA ) in muscle tissue in both high-fat fed mice and high-fructose fed mice. To study the association of LCACoAs with insulin resistance through two different diet-induced insulin resistant animal models. Methods Male Wistar rats (~300g) were fed a standard chow (Con), Fru (~34.5%) or Fat (~60%) diet for 3 weeks. Fasting blood glucose, plasma insulin, HOMA index, plasma triglyceride, triglyceride content and LCACoAs in muscle tissues were measured. Results Compared with control group, blood glucose, plasma insulin, HOMA-IR index, plasma triglyceride, triglyceride contents and total LCACoA content in muscle tissues were significantly increased in both high-fat group and high-fructose group. Total LCACoA contents in muscles were positively correlated with HOMA index. Conclusion Whole-body insulin resistance and LCACoAs aumulation can be induced by both high-fat feeding and high-fructose feeding. LCACoA content in muscle is associated with the development of insulin resistance.

Key words: long chain acyl-CoA, triglyceride, insulin resistance, muscle