Abstract: Objective To study the effect of protoporphyrin disodium (NAPP) on activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in liver tissue of mice with acute hepatic injury induced by carbon tetrachloride (CCl4). Methods 60 ICR mice were randomly divided into normal control group, CCl4 model group, bifendate pills group, NAPP low dose group, NAPP medium dose group and NAPP high dose group. Mice of each treatment group were injected different drugs by intragastric administration continuously and then a single dose of CCl4 was given to establish acute hepatic injury model. 16 hours later, blood was collected by picking eyeballs to detect the activities of ALT and AST in serum, liver was taken out to detect the activity of SOD and the content of MDA in liver tissiue of each group. Results Compared with the normal control group, the activities of ALT and AST in serum of the CCl4 model group were (1879±1219) U/L and (2210±1585) U/L, there has a significant difference (P<0.01); The activities of SOD and MDA in the liver tissues of the bifendate pills group, NAPP low dose group, NAPP medium dose group and NAPP high dose group were (207.61±16.02), (184.35±13.42), (190.88±17.77), (199.38±14.43) U/mgprot and (1.08±0.15), (1.35±0.26), (1.07±0.16), (0.92±0.18) nmol/mgprot, respectively, which have statistical sense compared with model group (P<0.05～P<0.01). Conclution NAPP can effectively prevent the decrease of SOD and increase of MDA in liver tissues of mice with acute hepatic injury, this shows NAPP has a good effect of protecting liver and decreasing enzymes on mice with acute hepatic injury induced by CCl4.

Key words: protoporphyrin disodium, carbon tetrachloride, alanine aminotransferase (ALT), aspartate aminotransferase (AST), superoxide dismutase (SOD), malondialdehyde (MDA)