Table of Content

    05 August 2011, Volume 31 Issue 8
    Expression and Relationship of PDCD5, MDM2, Tip60 and P53 in Esophageal Squamous Cell Cancer
    Wei SUN Xiu-min MA Jian-bing DING Hui GUO Yue-jie ZHU Lan-jun XU Jing-ping BAI
    2011, 31(8):  847-852. 
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    Objective To study the relationship of the expressions of PDCD5, MDM2, Tip60 and P53 in esophageal squamous cell cancer in Xinjiang. Methods RT-PCR was used to detect the mRNA of PDCD5, MDM2 and Tip60. The PDCD5, MDM2, Tip60 and P53 protein expressions were determined by using immunohistochemisry staining method. Results The positive incidence of PDCD5, MDM2 and Tip60mRNA expressions in 40 cases of the esophageal cancer tissues were 80.0﹪、65.0﹪、97.5﹪.The expression rate of MDM2mRNA in esophageal cancer was significantly higher than in normal esophagus mucosa and para-cancer tissue (P <0.05). Semi-quantitative analysis showed expression of PDCD5, MDM2 and Tip60mRNA have statistical difference in esophageal cancer and normal esophagus mucosa (P<0.05).Expression rate of PDCD5, MDM2 and P53 proteins were lower in esophageal cancer than that in normal esophagus mucosa(P <0.05).The positive expression rate of PDCD5 protein in esophageal cancer were significantly correlated with tumor differentiation (P<0.05). The expression of Tip60 and P53 protein,PDCD5 and MDM2mRNA were significant correlation(P <0.05). Conclusion It was speculated that there may be have a common transcription mechanism which constructed multiple pathways in cancer among PDCD5、MDM2、Tip60 and P53.
    M1-receptor involved in Orphanin FQ-induced the inhibition of delayed rectifier potassium current in rat cerebral parietal cortical neurons
    Xu YANG Li-hui QU Hua TIAN Lan-wei CUI Peng WANG Li-ying SONG Yu-rong LI
    2011, 31(8):  853-857. 
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    Objective To study the effect of M1 and M3 receptors’ antagonists on the inhibition of delayed rectifier potassium current ( IK ) by nociceptin/orphanin FQ ( N/OFQ ) in acutely dissociated rat cerebral parietal cortical neurons. Methods Using whole-cell patch-clamp technique, recording effects of different subtypes of M receptor on the inhibition of IK by N/OFQ in rat cerebral cortical neurons. Results ( 1 ) N/OFQ inhibited IK in acutely dissociated rat cerebral parietal cortical neurons, and the inhibition rate was ( 30.8±5.6 )% ( P < 0.01 ); ( 2 ) PIR M1 receptor antagonist antagonized the inhibitory effect of N/OFQ on IK and the drop of currents varied from the control value of ( 30.8±5.6 )% to ( 15.5±2.5 )% ( P < 0.05 ); ( 3 ) M3 receptor antagonist 4DAMP has not obvious effects on the inhibitory effect of N/OFQ on IK. Conclusion M1-receptor involved in the inhibition of N/OFQ on IK in acutely dissociated rat cerebral parietal cortical neurons.
    Influence on EGF and TGFβ1 signal in hyperoxic fetal rat alveolar epithelial type 2 cell injury
    Zhi-hui RONG Yan CHEN Rui-yan SHAN Wei LIU Li-wen CHANG
    2011, 31(8):  858-863. 
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    Objective To study imbalance between EGF and TGFβ1 signal in fetal rat alveolar epithelial type 2 cell exposed to oxygen. Methods Primary fetal rat type 2 alveolar epithelial cells were randomized divided into 2 groups: air group and O2 group. Cell apoptosis were measured by FACS. Sprouty2、EGF receptor、phospho-EGF receptor(Tyr1148)、phospho-EGF receptor(Tyr845)and TGF-βⅡreceptor were was tested by western blot. Sprouty2 mRNA were measured by real-time PCR. Results Compared with air group, there was increased cell apoptosis in O2 group according to FACS. Sprouty2 mRNA and protein were increased in O2 group than air group. EGF receptor、phospho-EGF receptor(Tyr1148)、phospho-EGFreceptor(Tyr845)were decreased in O2 group compared with air group while TGF-βⅡreceptor expression was increased in O2 group compared with air group. Conclusion EGF which can decrease Sprouty2 expression is downregulated in fetal rat alveolar epithelial type 2 cells exposed to oxygen. TGFβ1 which can increase Sprouty2 expression is upregulated in fetal rat alveolar epithelial type 2 cell exposed to oxygen. Both of these 2 mechanisms cause further rising of Sprouty2. We think this is very important in BPD.
    Homocysteine inhibits activity of eNOS in cultured human umbilical vein endothelial cells
    Hua ZHONG Qing-hua HU Hui-min ZHANG Dan ZHAO Zhi-ping SUN Zeng-chun LI Fang HE
    2011, 31(8):  864-868. 
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    Objective To study the mechanism of homocysteine(Hcy)at different concentrations and action times on eNOS activity in cultured human umbilical vein endothelial cells(HUVECs), both in base and calcium ionophore activated state. METHODS HUVECs were collected and cultured to the second passage.Then the second passage of HUVECs were cultured with different concentrations Hcy and in different action times. The expression of HSP90,Akt,Caveolin-1,eNOS,P-eNOS proteins were detected by western blot and the expression of Caveolin-1,eNOS,DDAH mRNA by RT-PCR.RESULTS (1) Compared with control group, both in base and calcium ionophore activated state. The expression of Caveolin-1 group increased obviously(p<0.05) in concentration and time-dependent manner but the expression of Akt showed a decrease(p<0.05). The expression of eNOS did not change significantly(p﹥0.05);(2) There was no difference in the expression of HSP90,eNOS,P-eNOS protein and mRNA among every group. DDAH mRNA among every group too..CONCLUSIONS There was no relationship between decreasing activity of eNOS induced by Hcy and eNOS expression, Hcy probably can increase the association of Caveolin-1and eNOS by enhancing the expression of Caveolin-1,and reduce the complex formation of Caveolin-1, Akt,Caveolin-1 in HUVEC both in base and activated state, which can decrease the activity of eNOS.
    The anti-inflammatory valument and inhibition effects of erythromycin on TREM-1 on U937 cell
    Jin TONG Dao-xin WANG
    2011, 31(8):  869-874. 
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    Objective To comprehend the effect of erythromycin on TREM-1, and investigate the mechanism on inflammation regulation of it. Methods U937 cells were cultured and separated into four groups: control group, LPS group, LPS+ TREM-1/FC fusion protein group and LPS+ erythromycin group. The cytokines, TNF-α, IL-6, IL-1β and sTREM-1, were measured by ELISA. The expression of membrane receptor and gene of TREM-1 were measured by flow cytometry and RT-PCR respectively. Results The top point of membrane expression of TREM-1 was (0.65±0.05)and (0.72±0.04)in TREM-1/FC fusion protein group and erythromycin group respectively, which was lower than that in LPS group(0.98±0.12)(P<0.01). They couldn’t inhibit the expression of the gene, compared with LPS. The secretion of these cytokines was inhibited by them obviously, under LPS stimulation. The depressant effect of TREM-1/FC fusion protein was stronger than that of erythromycin, on sTREM-1 (P<0.01) and IL-6 (P<0.05), but the result was contrasted on TNF-α, IL-1β(P<0.05). Conclusions Erythromycin could inhibit the expression of TREM-1, which would depress the inflammatory reaction, and has the analogous clinical anti-inflammatory effect as TREM-1/FC fusion protein.
    Population genetic studies on nine head-face characters of Yi in Sichuan
    Ying LIAO Xia LI Li-dan XIONG Lian-bin ZHENG Xing-hua ZHANG Ling-ling SHI Qiong WANG
    2011, 31(8):  875-878. 
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    Objective To understand the genetic characters of Yi’s head-face in Liangshan of Sichuan. Methods Nine genetics indexes, including mongoloid fold, eyefold of the upper eyelid, front tooth type, nasal profile, nostril form, chin projection, lope type, hair point of the forehand and hair form were investigated for 313 middle school students (204 males and 109 females) in Yi by using cluster random sampling method. Results (1) The rate of Mongolia fold is 58.47%, the rate of eyefold of the upper eyelid is 85.94%, the rate of shove-shaped front tooth type is 95.85%,the rate of umbonate nasal profile is 2.87%, the rate of wide nostril is 77.32%,the rate of protruding chin is 4.15%, the rate of ear lope is 72.84%,the rate of pointed hair point on forehead is 37.70% and the rate of curly hair form is 23.00% in Yi. (2) Eight indexes showed no sexual difference except for the rate of point of the forehand. Conclusions There are differences between Yi and other nationalities in genetic characters of head- face.
    Increased Apoptosis in amygdala neurons of depression model rats
    Yan-xia TIAN Hai-tao WANG Ran LI Quan KAN Jun-ling GAO
    2011, 31(8):  879-883. 
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    Objective To observe the expression of Cytochrome C and apoptosis-related gene bax in amygdala of depression model rats. Methods Male Wistar rats were randomly divided into control group and model group. Chronic forced swimming stress was carried out to set up the rat depression model. TUNEL-staining and double-labeled flow cytometry (FCM) were employed for the detection and quantification of the apoptotic cells in the amygdala. The expression of Cytochrome C and gene bax was detected using Western blotting and RT-PCR. Results The consumption of sucrose, preference of sucrose, erect quantity and escape latency of model rat were signficantly decreased compared with of the normal group (P<0.01, P<0.05). The percentage of TUNEL-positive cell, the apoptotic cell rate and the expression of Cytochrome C and gene bax of model group were higher than those of control group respectively (P<0.01). Conclusion Apoptosis was increased in amygdala neurons of depression model rats, and bax is up-regulated and promotes the releasing of Cytochrome C.
    Effects of AFP Gene Silencing on Proliferation of Hepatocellular Carcinoma Cell Line EGHC-9901
    2011, 31(8):  884-888. 
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    Objective to observe effects of AFP gene silencing by siRNA on proliferation of hepatocellular carcinoma cell line EGHC-9901. Methods siRNA expressing plasmid aimed at AFP gene was firstly established and subsequently transfected into hepatocellular carcinoma cell line EGHC-9901; after G418 positive clone selection for 4-5 weeks, AFP expression both of mRNA and protein were detected by semi-quantitative RT-PCR and western blot; cells then were divided into three groups: experimental group, AFP-siRNA transfected; vector control group, empty vector transfected; blank group, untreated. MTT and flat plate clone formation assay were applied to evaluated cell proliferation, and flow cytometry was employed to observe cell cycle. Results siRNA expressing plasmid aimed at AFP gene was successfully transfected into hepatocellular carcinoma cell line; AFP expression was almost completely inhibited. The experimental group proliferated at a significantly lower speed than the positive control group; The experimental group exhibited less clones with diameters more than 75μm than the positive control group, p<0.05; flow cytometry manifested approximately 20% more cells of the experimental group within G1 phase than those of the positive group, indicating that inhibition of AFP expression may cause G1 phase arrest. Conclusion siRNA expressing plasmid aimed at AFP gene was successfully stably transfected into hepatocellular carcinoma cell line EGHC-9901; AFP gene silenced by siRNA induces growth and colony formation inhibition of hepatocellular carcinoma cell line EGHC-9901.
    Enhanced expression of phosphorylated JNK of cochlea ototoxicity by amikacin -induced in guinea pig
    Shuang-yue LIU Ai-mei WANG Tao XU
    2011, 31(8):  889-893. 
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    Objective To investigate the effect of amikacin (AMK) on the expression of phosphorylated JNK (p-JNK) in hair cells of guinea pig cochlea and the mechanism of AMK ototoxicity. Methods Guinea pigs were assigned to control group, AMK 3, 7 and 11 days group. Animals from AMK groups were daily received intramuscular injection of AMK (400 mg/kg) , while control animals were received an equivalent volume of saline. Auditory brainstem response (ABR) test and tetramethylrhodamine isothiocyanate (TRITC) -labeled phalloidine staining were used to observe the auditory function and hair cells morphology. immunohistochemistry and imaging analysis technique and Western blot were used to detect the expression of p-JNK in the cochlea. Results The hair cells missing was increased in the basal turn of cochlea and developed to the second turn. The expression of p-JNK was greater remarkably in AMK groups. And the ABR thresholds shifts increase significantly than the control group (P<0.01) . Conclusion JNK signal pathway might participate in AMK- induced ototoxicity.
    PI3K/Akt pathway mediates the anti-oxidative injury induced by H2O2 preconditioning in PC12 cell
    2011, 31(8):  894-899. 
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    Objective To explore whether PI3K/Akt pathway play a role in H2O2 preconditioning-induced adaptive cytoprotection. Methods setting up the experimental model of H2O2 preconditioning against injury induced by H2O2 at high concentration in cultured PC 12 cells. The viability of cells was measured by MTT assay. The activity of lactate dehydrogenase (LDH) was assessed by colorimetry. The percentage of apoptotic cells was estimated by flow cytometry (FCM) with propidium iodide stain. The levels of akt were detected by Western blot. Results Preconditioning of H2O2 at 100 μmol/L for 90 min significantly protected PC 12 cells from 300 μmol/L H2O2-induced injury, increasing the cell viability from (50.2±4.6)% to(83.8±3.5)% , reducing the activity of LDH from(103±10.21)% to (68.5±5.34)%,and decreasing the rate of apoptotic cells from (65.5±4.1) % to (37.1±2.3) % (P<0.01). H2O2 preconditioning induced expression of p-akt, and ly294002 (an inhibitor of PI3K) blocked expression of p-akt induced by H2O2 preconditioning. Simultaneously ly294002 markedly reduced the anti-cytotoxicity and anti-apoptosis induce by H2O2 preconditioning. Conclusion H2O2 preconditioning induced activation of akt via PI3K-dependent way and activation of PI3K/Akt pathway mediated in adaptive cytoprotection induced by H2O2 precondition.
    Diagnostic value of multiple tumor marker protein biochip for patients with cholangiocarcinoma
    Jiang HE Jian-bing DING Wu-zhong YU Hong-yun ZOU
    2011, 31(8):  900-904. 
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    Objective To evaluate the diagnostic value of multiple tumor markers protein biochip (C-12) for patients with cholangiocarcinoma. Methods The serum levels of 12 tumor markers (TMs), including CA199, NSE, CEA, CA242, Ferritin, β-HCG, AFP, f-PSA, PSA, CA125, CA153 and HGH, were measured in 26 cholangiocarcinoma patients, 52 benign disease patients of digestive system and 65 normal controls. The most relevant TM and the combinations of TMs to the improvement of diagnosis were determined. Results The positive rates of CA199, CEA, CA242, Ferritin, AFP, CA125 and CA153 in the cholangiocarcinoma patients had significantly higher than those of the benign disease patients and normal controls (P<0.01). The serum levels of CA199, NSE, CA242, Ferritin, β-HCG, AFP, CA125, HGH and CA153 in the cholangiocarcinoma patients had significantly higher than those of the benign disease patients and normal controls (P<0.05~0.01). Among all the 12 TMs, the single best TM was CA199 and gave the sensitivity, specificity and validity of 69.2%, 96.9% and 89.0% for the cholangiocarcinoma, respectively.The best combination of 3 TMs (CA199+CA125+CEA) had not statistically significant comparied with C-12 and yielded a sensitivity of 91.7%, a specificity of 87.5% and a validity of 89.6%. Conclusions The multiple tumor markers protein biochip has a relatively high value in the diagnosis of cholangiocarcinoma. The combination detection of 3 TMs (CA199+CA125 +CEA) could replace 12 TMs of C-12 and may be the economical and effective in the diagnosis of cholangiocarcinoma.
    Glycyrrhizin relieves in rats pulmonary fibrosis by Bleomycin
    Zong-bao YIN Ying LI Chao DENG Yu-fang CAO
    2011, 31(8):  905-908. 
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    Objective 1.To investigate effects of glycyrrhizin in rat model of pulmonary fibrosis produced by Bleomycin..Methods Sprague Dawley rats were randomly divided into pulmonary fibrosis group,NS group,DXM、 glycyrrhizin low dose、meta-dose、high dose treatment group. All pulmonary fibrosis rats in the treatment groups were intraperitoneal injection after 28 days and last for 14 days. At the 15th days, the left lung were measured by immunity class TGF-β1,IFN-γ;the right lung was made into slice which cell apoptosis was detected. The blood-serum was measured by ELISA IFN-γ and IL-4. Results 1. The pulmonary alveolitis and fibrosis of BLM group were signifieantly highter.TGF-β1 protein expression in bronehiolar epitheliums of BLM group. was stronger and more significant deviation than in every one group(p﹤0.01). 2. The IL-4 level of BLM group was higher than all the treatment groups,but the IFN-γ level was contrary(P<0.05). 3. Strong IFN-γ protein expression was deteeted in glycyrrhizin treatment groups. IFN-γ protein expression in glycyrrhizin low dose group was more significant deviation than in the other group(p﹤0.01). Conclusion The glycyrrhizin less TGF-β1 protein expression and IL-4 level of pulmonary fibrosis rats,but IFN-γ was contrasty. Those evidenees suggest that glycyrrhizin has strong ability to antifibrosis.
    Relationship between STK15 Phe31Ile Polymorphism and Risk of Colorectal Cancer: a Meta-analysis
    Shen YANG Sheng-yu DUAN Rong ZHONG Li LIU Li LIU Shao-fa NIE Xiao-ping MIAO
    2011, 31(8):  909-913. 
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    Objective To determine whether STK15 Phe31Ile Polymorphism contributes to genetic susceptibility to colorectal cancer, through summarizing the results of published works in this field by a meta-analysis. Methods Pubmed、EMbase、SCI、Web of Science、CNKI、VIP and some other databases were searched for literatures published from January 2000 to April 2010. Articles were identified using the Medical Subject Headings term “STK15”、“polymorphism”、 “aurora-A”、“colorectal cancer”、“colorectal carcinoma”. Case control studies involving unrelated subjects and genotype frequencies in control group consistent with Hardy-Weinberg equilibrium were included. The software Stata(Version10.0) was used for meta-analysis. Results Six studies including 5158 patients and 4608 controls met the selection criteria. The combined OR of susceptibility to colorectal cancer with Phe/Ile and Ile/Ile compared to Phe/Phe were 1.01(95%CI: 0.93~1.10) and 1.18 (95%CI: 0.98~ 1.42); and their responding P values were 0.81and 0.081 respectively. Conclusion The findings suggest STK15 Phe31Ile Polymorphism is not correlated with susceptibility to colorectal cancer. Further study is needed to confirm STK15 Phe31Ile polymorphism and susceptibility to colorectal cancer in Chinese population.
    Remifentanil-induced hyperalgesia in spinal dorsal horn and the inhibition of lidocaine in rats
    Wei-hua CUI Hong TAN Ru-quan HAN Shu-ren LI Jun-fa LI
    2011, 31(8):  914-918. 
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    Objective To determine the involvement of conventional protein kinase Cgamma (cPKCγ) in the inhibitory action of lidocaine on remifentanil-induced hyperalgesia of rats after propofol-remifentanil-based anesthesia. Method Male Sprague-Dawley rats were allocated into the following groups randomly: propofol only (Group P), propofol+remifentanil (Group R), propofol+remifentanil+lidocaine (Group RL), and propofol+lidocaine (Group L). Cumulative pain score and withdrawal response to mechanical stimulation, immunoblotting, and immunofluorescence were applied to observe remifentanil-induced hyperalgesia and cPKCγ membrane translocation. Result 1) Cumulative pain score in Group R were higher than the other groups on postanesthesia 120min (P<0.05). While it were similar among Group P, RL and L. 2) Withdrawal threshold on the ipsilateral side to incised plantar in Group R were lower than the other groups (P<0.05) on posteanesthesia 120min. 3) Both immunoblotting and immunofluorescence demonstrated that the membrane translocation of PKCγ in dorsal horn neurons increased in propofol-remifentanil anesthetized animals and systemic lidocaine could inhibited it. Surgery procedure won’t affect the membrane translocation of PKCγ in dorsal horn neurons shortly after anesthesia. Conclusion Increased PKCγ membrane translocation in spinal dorsal horn involves in remifentanil-induced hyperalgesia, which was inhibited by systemic lidocaine and may contributes to reduced postoperative pain in rats after remifentanil-based anesthesia.
    The impact of anti-anxiety and antidepressant treatment on the blood glucose profile in Type 2 diabetes
    Ling LI Dong-mei NI
    2011, 31(8):  919-921. 
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    Objective To investigate the impact of anti-anxiety/antidepressant treatment on the blood glucose profile in patients with type 2 diabetes.Method Sixty type 2 diabetes patients with a tendency of anxiety or depression, which was diagnosed according to the Zung Self-Rating Depression Scale (SDS) and Zung Self-Rating Anxiety Scale (SAS), were randomly assigned to received either anti-diabetic treatment solely,as the control group, or in combination with anti-anxiety or antidepressant therapy, as the intervention group. After 12-weak treatment, the changes in the index of anxiety (SDS)/depression (SAS), fasting blood glucose and HbA1C were compared between the two groups.Result Compared with that of before treatment , the SDS, SAS, FBG, and HbA1C were decreased significantly in both intervention group and control group, In intervention group , FBG decreased from 9.14±1.58mmol/L to 6.90±0.73 mmol/L, and HbA1C decreased form 9.88%±1.96% to 7.60%±1.00% respectively after treatment (P<0.05). In the control group, the corresponding parameters was 9.09±1.69mmol/L, and 9.68%±0.19% respectively before treatment, decreased to 7.60±0.81mmol/L,and 8.09%±0.83% respectively after treatment(P<0.05). The reduction in FBG and HbA1C was significantly greater in intervention group compared to control group. Conclusion: In type 2 diabetes patients with anxiety/depression tendency, anti-anxiety/antidepressant treatment can improve the blood glucose.
    Construction and identification of the recombinant adenovirus expressing BMP4-SiRNA
    Dan GUO Jia-yi HUANG Zhi-jun SUN
    2011, 31(8):  922-927. 
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    Objective To construct a recombinant adenovirus expressing siRNA target sites for human BMP4. Methods six pairs of oligonucleotides containing siRNA target sites for human BMP4 using Dharmacon’s siDESIGN program were subcloned into the Sfi I site of pSOS,which has been inserted the coding regions of human BMP4,resulting in pSOS-sirBMP4.Then the pSOS-sirBMP4 were transfected into HEK-293 cells, and GFP signal levels were used to assess the silencing efficiency of different siRNA target sites. Four pairs of oligonucleotides were chosed and subcloned into shuttle plasmid pSES-HUS and authenticity of PCR amplified sequences and the oligonucleotide cassettes were verified by DNA sequencing. The correctly identified recombinant plasmid was linearized with Pme1 and transformed into BJ5183-Ad-easy competent cells containing adenovirus backbone vector to produce recombinant adenovirus DNA by homologous recombination. Then the recombinant adenovirus DNA was linearized with Pac1 and transfected into 293 cells to make adenovirus. the silencing efficiency of recombinant adenovirus was assess by RT-PCR and Western blot in MDA-MB-231 cell line. MTT assay was used to detect the proliferation of MDA-MB-231 cells transfected with pAd-sirBMP4. Results Four pairs of oligonucleotides with high silencing efficiency were chosed and subcloned into shuttle plasmid pSES-HUS to make recombinant adenovirus . After infected with the recombinant adenovirus BMP4-siRNA, the expression of BMP4 sharply decrease in the human breast cancer cell line MDA-MB-231 . Conclusion The recombinant adenovirus expressing siRNA target sites for human BMP4 has been successfully constructed and could be used to silence the expression of BMP4 in MDA-MB-231 cell line effectively.
    Clinical case disscussion (step by step)——ostalgia, chronic renal failure, M proteinemia
    Hua-xia YANG Shu-jie WANG Wen ZHANG Jun-ling ZHUANG
    2011, 31(8):  928-931. 
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    Analysis of 110 cases Epstein—Barr virus infection and related diseases
    Jun DAI Ling ZHANG Xiao-jing LI
    2011, 31(8):  932-935. 
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    Objective To explore the relativity of Epstein—Barr virus and diseases. Methods The related diseases of 110 Epstein—Barr virus infected patients with one or more than one of EBV IgG,IgA,IgM/VCA positive detected by immunoenzyme dyeing technique were analyzed retrospectively. Results There are 18 kinds related diseases in the Epstein—Barr virus infection of 110 cases. The most common disease caused by the Epstein—Barr virus infection is respiratory system infection. It accounted for 32.73%, followed by infections of hematologic system, digestive system, cardiovascular system, mononuclear phagocyte system and so on. Conclusion The Epstein—Barr virus infection may cause multiple system damage.
    Dynamics characteristics of ATP-sensitive potassium channels of guinea-pig airway smooth muscle cells
    Hong-yu SUN Fei ZOU Bo-xing LI Ming CHEN
    2011, 31(8):  936-937. 
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    Effects of dietary fat acids on p-eIF2α in liver tissues in rats
    Ping LI Guang-yao SONG Yong TANG Zi-jing WANG Wen-cong LI Bo-qing MA
    2011, 31(8):  938-940. 
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    Decreased expression of ADAM33 gene in asthma mice with the Budesolide treated
    Xiang-yu CHI Lai-cheng WANG Jing WANG Jian-ping WANG Yong-kang WANG
    2011, 31(8):  941-942. 
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    Construction of Eukaryotic Expression Vector of Apolipoprotein M and its Effect on HBV Replication
    Xing-hui LIU Cheng-liang ZHU Hui SONG Yan WANG Yi LI
    2011, 31(8):  943-944. 
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    Selectively anti-oxidative roles of hydrogen
    Xiao CHEN Qiao ZUO Xue-jun SUN
    2011, 31(8):  945-947. 
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    Hydrogen can selectively reduce cytotoxic oxygen radicals. Various experiments have demonstrated that when oxidative stress occurs and cytotoxic oxygen radicals are over produced, i.e. ischemia/reperfusion injury, ischemia-hypoxia injury and acute inflammation, administration of hydrogen significantly suppress the oxidative injuries by selectively neutralizing cytotoxic oxygen radicals.
    The relationship between haptoglobin polymorphism and cardiovascular complication of diabetes mellitus patients
    Ping WANG Hong LIU Yi-shi LI Yu-qing LIU
    2011, 31(8):  948-950. 
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    Haptoglobin, an acute phase protein, can be found in plasma of human-being and other mammals. It plays important role in anti-infection, reparation of injured tissue, homeostasis. Different from other mammals, human-being have three different HP genotypes, HP1-1,HP2-2,HP2-1.Many studies have show that HP gene had been established as the susceptibility gene of diabetic cardiovascular disease. The review will introduce the structure and physiologic function of HP, summarize the basic and clinical researches about relationship between the HP genotype and prevalence of cardiovascular diseases especially the cardiovascular complication of diabetic patients, prospect the prevention of cardiovascular complication of HP2-2 diabetic mellitus individual.
    Progress on dendritic cells in rheumatoid arthritis
    Qi WANG Xue-jun ZENG
    2011, 31(8):  951-954. 
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    Rheumatoid Arthritis is a systematic inflammatory auto-immune disease characterized by synovial hyperplasia and progressive joint destruction.It is thought that RA is caused by the presentation of self antigen by APC. Dendritic cells are antigen handing cells that regulate a wide spectrum of immune responses. They not only initiate immune responses but also induce immune tolerance. This character may apply a new sight for treating RA clinically.
    Progress of key genes involved in renal cell carcinoma
    Qing-hai WANG Bing-bing SHI
    2011, 31(8):  955-958. 
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    Renal cell carcinoma is a type of multi-gene-related cancer, different subtype with a different histology, having a different clinical characteristics, responding differently to therapy and caused by different genes. Therefore, studying the related genes of cancer of the kidney has significant implications not only in depth understanding of the pathogenesis of renal cell carcinoma, but also in clinical application. This article reviews the major involved kidney cancer genes,such as VHL, PBRM1, MET, FH, BHD, etc., and analyzes their value in diagnosis and treatment of renal cell carcinoma.