Abstract: 【Abstract】 Objective To characterize and purify the allergenic components of Bischofia polycarpa pollen. Methods Pollen extract from Bischofia polycarpa pollen prepared and analyzed by SDS-PAGE and proteins band were visualized by staining the gel with Coomassis blue. Antigenic properties of the separated proteins were analyzed by immuno-blotting. Ion-exchange chromatography with DE-52 was used for the purification of the antigens. Then purified products were characterized by western-blotting. Results For the pollen extract of Bischofia polycarpa, 18 protein bands was in SDS-PAGE. Two of these protein bands, with a molecular weight of 12 000 Mr ang 14 000 Mr, showed immunoreactivity with IgE in the sera from patients with pollen allergy of Bischofia polycarpa. Result from the ion-exchange chromatography indicated that the two pollen allergens (12 000 、14 000 Mr) from Bischofia polycarpa were eluted in the second peak. Conclusion Pollen allergens form Roystonea regia were purified and characterized.The result will provide a basis for the diagnosis and treatment of allergy to the pollen allergens form Bischofia polycarpa