Table of Content

    20 September 2009, Volume 29 Issue 9
    Regulation of acylation stimulating protein on expression of lipid droplets associated protein TIP47 through PLC signal pathway
    Xiao-yan LIANG; Hui-ling LU; Xiu-fen HU; Jing WU
    2009, 29(9):  897-900. 
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    Objective To study the effect of acylation stimulating protein(ASP) on lipid droplets associated protein TIP47 during the differentiation process of adipocytes and the change of this effect after blocking PLC signal pathway. Methods (1) To treat 3T3-L1 adipocytes at indicated time point during differentiation with ASP、U73122、U73122 and ASP respectively, including control group in the same time. (2) To examine the mRNA and protein expression levels of TIP47 by RT-PCR and Western blot technique respectively. Results (1) Exposure to ASP for certain time resulted in the obvious increase of TIP47 gene and protein expression in 3T3-L1 adipocytes, compared with the control group; (2) Exposure to U73122 which is blocking agent of PLC signal pathway for certain time resulted in the decrease of TIP47 gene and protein expression in 3T3-L1 adipocytes, compared with the control group; (3) Gene and protein level of TIP47 in adipocytes treated with both U73122 and ASP was significant lower than that in adipocytes only treated with ASP, while was higher than that in adipocytes only treated with U73122. Conclusions PLC signal pathway plays a role in signal transduction of regulation of ASP on expression of TIP47. These results will further deepen the understanding of adipogenesis of ASP on molecular level, and offer some new thinking for etiology, prevention and treatment of obesity.
    A novel mouse model of pulmonary allergic inflammation sensitized and challenged with house dust mite Dermatophagoides farinae
    Hua-xia CHEN; Jin-ming GAO; Lei JIANG; Li NIE; Zi-jian GUO
    2009, 29(9):  901-904. 
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    Objective To explore the method of establishing pulmonary allergic inflammation model in C57BL/6 mice sensitized and challenged with house dust mite Dermatophagoides farinae (Der f). Methods C57BL/6 mice were divided into control group and treatment group. The mice of treatment group were sensitized by intra peritoneal injection of house dust mite extracts at day 1, 3, 5, 7, 9 and 11. Then they were exposed to aerosolized allergen at day 13, 16, 19, 20 and 21. Physiological saline instead of house dust mite extracts was used in control group. All mice underwent pulmonary lavage in 24h after the last time of exposure to aerosolized allergen challenge. Pathological manifestation of the lung, cell counts and classification were studied and IL-4 and IFN-γ levels in BALF were detected by ELISA. Cells from spleen were cultured for 3d with house dust mite extracts, IL-4 and IFN-γ in supernatants was measured by ELISA. Results There was pulmonary eosinophilic inflammation in the mice treated with house dust mite extracts compared with control group. Total cells, lymphocytes, eosinophils and the level of IL-4 in BALF from treated mice increased significantly,while IFN-γ in BALF decreased. The level of IL-4 in cultured splenocyte supernatants also increased significantly,while IFN-γ in supernatants decreased. Conclusions A pulmonary allergic inflammation model of C57BL/6 mice has been established by sensitized and challenged with house dust mite Der f.
    Deletion of gr/gr-DAZ1/DAZ2 gene and male infertility
    Zhou-cun A; Li XU
    2009, 29(9):  905-907. 
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    Objective To study the relationship between the gr/gr-DAZ1/DAZ2 gene deletion and male infertility in Chinese population. Methods Distribution of gr/gr-DAZ1/DAZ2 gene deletion was investigated in 364 infertile men and 287 fertile controls using polymerase chain reaction(PCR) and fragment length polymorphism analysis (RFLP). Results gr/gr-DAZ1/DAZ2 gene deletion was detected in both infertile men and controls, but the incidence of gr/gr-DAZ1/DAZ2 gene deletion in infertile men was significantly higher than that in controls (%9.3 vs. 2.8%, P<0.001). Conclusion Deletion of gr/gr-DAZ1/DAZ2 gene is not a direct etiology but a risk factor of male infertility, suggesting that it is not essential to detect this deletion during gene diagnosis for male infertility in Chinese.
    Obtaining fetus of rhesus monkey with the combination of misoprostol and mifepristone
    Li ZHAO; Yuanyuan HAN; Donghong TANG
    2009, 29(9):  908-910. 
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    Object To establish a safe and effective method to obtain fetus of rhesus monkey. Method The pregnant days of rhesus monkey was identified by B ultrasonography, and the pregnant rhesus monkeys in different stages of pregnant were chosen randomly. Mifepristone was subcutaneously injected to pregnant rhesus while misoprostol was put to their posterior fornix. The effect of this method was evaluated by the results of bishop score ,the rate of pregnancy termination and the quality of total RNA extracted from brain samples of rhesus fetus. Result The scores of bishop score and rates of fetus delivery of experiment group were both higher than control group (P<0.05) ,and the quality of total RNA extracted from brain samples of rhesus fetus are well. Conclusion Combined application of misoprostol and mifepristone can effective and safely obtain samples of rhesus fetus.
    Study on the function of Transgenic cell line as a feeder layer on UCB CD34+ HSPC ex vivo amplification
    Zhi-qing HU; Ji-cheng YANG; Wei-hua SHENG; Ying-ying JING; Li MIAO; Ri ZHANG; Nan-kang ZHU; Jing-cheng MIAO
    2009, 29(9):  911-915. 
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    Objective To establish the transgenic cell line of hOSM gene and observe its effect on the proliferation of umbilical cord blood(UCB)CD34+ HSPC as a feeder layer. Methods A transgenic cell line was established by molecular biology method and CD34+ HSPCs were separated by magnetic-activated cell sorting. After 7 days' co-culture, the amplification effect was evaluated by flow cytometry. Then CD34+ cells stained by CFDA SE were injected into the SCID mice with sublethally irradiation and the effect of transplation was observed by fluorescence microscopy and RT-PCR. Results The number of CD34+ cells increased by 9.6 times and the expression rate of homing-ralated molecules was still higher than 50%. Four weeks after transplantion, CD34+ cells were found in bone marrow of SCID mice. Conclusion The transgenic cell line of hOSM gene as a feeder layer can effectively amplify UCB CD34+ HSPC ex vivo and suppress its differentiation. Meanwhile, they have high transplantion efficacy and haematogenesis activity.
    Establishment of cardiomyocyte from mouse embryonic stem cell-derived embryoid bodies
    Jie QIN; Zhao-ying XUE; Xin GUO; Yao-ting GUI; Zhen-dong YU; Zhi-ming CAI
    2009, 29(9):  916-919. 
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    Objective To establishment a defined three-dimensional culture system of cardiomyocytes from mouse embryonic stem cell-derived embryoid bodies (EBs). Methods The EBs were derived from mES by suspending culture and were plated to Matrigel? Matrix three-dimensional system. The mouse fibroblast cells served as negative control to identify genes expression of contracting EBs. Results The first beating was irregular after 5 days of culture, but became consistent after 3 days with at an average frequency of 80-100 beats per minute. mES expressed cardiac specific protein of cTnT,Desmin and Actin. The expression of genes associated cardiomyocytes differentiation as cTnT, NKX2E, GATA-4 and MLC-2v were detected by RT-PCR. Conclusion By using the Matrigel? three-dimensional system, mES-derived EBs could spontaneously differentiate to cardiomyocytes effectively, and possess necessary biological characters of cardiomyocytes .
    Association analysis of XRCC1-Arg399Gln polymorphism with thyroid-associated ophthalmopathy
    Wen-hao MA; Rui QIN; DShao-kang TENG
    2009, 29(9):  920-923. 
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    Objective To investigate the association of XRCC1-Arg399Gln polymorphism with hyroid-tassociated ophthalmopathy (TAO ) . Methods XRCC1-Arg399Gln polymorphism was determined by PCR-RFLP in 182 patients with TAO and 182 healthy subjects. Results The significant difference of the allelic frequency distribution was found between TAO and control group(P〈0.001),The frequency of A allelic in TAO was higher than that in control group .Compared with subjects carrying GG allele, the risk of TAO for the individuals carrying GA+AA allele increased(OR 1.863,95%CI 1.228~2.826,P<0.05). The OR of TAO for individuals carrying A allele or cigarette smokers was respectively 1.343(95%CI 0.764~2.36) and 2.0274(95%CI 1.121~3.667).But The combination of the above two made the OR of TAO up to 4.981(95%CI 2.697~9.1998). Conclusion Allele A in XRCC1Arg399G1n may be associated with TAO. Potential interactions were found among XRCC1-Arg399G1n polymorphisms and cigarette smoking.
    Microsatellite DNA analysis of inbred mouse somatic cell nuclear transfer blastocysts
    Min QIN; Min HE; Qiang XUAN; Lin-jian MO; Hua WU; Zeng-nan MO
    2009, 29(9):  924-928. 
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    Objective To identify the source of the somatic cell nuclear transfer blastocysts , microsatellite DNA assay was developed. Methods DNA isolation from SCNT blastocyst,donor BALB/c mouse, recipient C57BL/6 mouse and KM mouse are tested by nested polymerase-chainreaction(PCR) analysis. Primers designed for four specific microsatellite locus(D3Mit28, D11Mit258, D12Mit136, D14Mit50)were employed. The amplified PCR products from the final reaction was analyzed by agarose gel electrophoresis and visualized with ethidium bromide staining. Results Amplification of genomic material by nest PCR represents the most sensitive method for the detection and might be detected successfully even though very low DNA copies. Microsatellite DNA analyses examining four loci confirm that all the SCNT blastocysts were genetically identical to the donor mouse. Additionally, the SCNT embryos are not genetically related to the respective recipient mouse. Furthermore sequences of SCNT blastocysts are diferent from the control KM mouse. Conclusion Our results prove that the nucleus of somatic cell nuclear transfer blastocysts come from somatic nucleus of donor BALB/c mouse.
    Effect of 8-nitrochyrsin on the proliferation and apoptosis of HL-60 cells in vitro
    Jun YUE; Shi-hua WU
    2009, 29(9):  929-932. 
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    Objective To investigate the effect of 8-nitrochyrsin on cell proliferation and cell apoptosis of HL-60 cells in vitro. Methods 8-nitrochyrsin on proliferation of HL-60 cells was detected by MTT assay; The trypan blue exclusion method was used to count the number of viable and dead cells, then the survival rate and growth curve were achieved; DNA ladder bands were observed by DNA agarose gel electrophoresis; HL-60 cell apoptotic percentage was detected by flow cytometry with PI staining. Results 8-nitrochrysin inhibited proliferation of HL-60 cells in a concentration- dependent manner; 8-nitro- chyrsin posses the inhibitory effect of the proliferation and growth of HL-60 cells; 8-nitrochrysin posses the significantly effect of induce apoptosis of HL-60 cells. Conclusion 8-nitrochyrsin posses the inhibitory effect of the proliferation and growth of HL-60 cells And significantly induce apoptosis of HL-60 cells.
    Effect of Edaravone on ERK1/2 Signal Pathway and Cell Apoptosis FollowingTraumatic Brain Injury in Rats
    Ya-ning ZHAO; Yin-xia DENG; Jian-zhong CUI; Yu-xin ZHANG; Jun-ling GAO
    2009, 29(9):  933-937. 
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    Objective To study the effect and potential mechanism of Edaravone on extracellular signal-regulated kinase 1/2 (ERK1/2) signal pathway and neuron apoptosis after traumatic brain injury(TBI). Methods Male Sprague-Dawley rats(n=120) were divided randomly into three groups:sham operation group (A group, n=24 ), traumatic group (B group, n=48), Edaravone treatment group (C group, n=48). TBI rat model was established accor ding to the description of Marmarou's diffused brain injury. At different time points (1, 6, 24, 48,72 h) after operation, the malondialdehyde (MDA) contents in cortex were measured with spectrophotometry, the qualities and quantities of p-ERK1/2, Bax and bcl-2 were detected by immunohistochemistry and Western-Blot, the quantities of neuron apoptosis were observed with TUNEL method. Results Compared with sham group,MDA contents(6~72 h post trauma),the expression levels of p-ERK1/2(1~48 h post trauma),the rates of Bax /bcl-2(6~48 h post trauma) and the apoptotic cells(6~72 h post trauma)in the cortex were significantly enhanced (p<0.05) following TBI;Compared with traumatic group,the above mentioned indexes in Edaravone treatment group were decreased obviously (p<0.05).Conclusions Edaravone can dramatically alleviate neuron apoptosis. The one of mechanisms is related to its scavenging oxygen free radical and down-regulation effect on ERK1/2 signal pathway.
    Dimethyl sulfoxide induced bone marrow mesenchymal stem cells differentiate into cardiomyocyte-like cells in vitro
    Hai-ping WANG; Lei ZHANG; Su-xia SHAO; Jing ZHAO
    2009, 29(9):  938-942. 
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    Objective To explore the optimal condition for the differentiation of rat bone marrow mesenchymal stem cells(MSCs) into cardiomyocyte-like cells in vitro by using DMSO.Methods SD rat MSCs were isolated from rat bone marrow and cultured, then induced by 1.0%DMSO for 24 h. The cultured cells were observed under phase-contrast microscope. The immunohistochemical technique and laser scanning confocal microscope (LSCM) were used for detecting the expression of desmin, α-sarcomeric actin and C-TnT. The ultrastructures were viewed with transmission electron microscopy.Results MSCs induced by DMSO could be identified by the positive staining for Desmin, α-Sarcomeric Actin and C-TnT.The number of cells stained positively in the 1.0% was visually larger than that of other groups. Transmission electron microscope showed that paralleled myofilaments and a lot of rough endoplasmic reticulum were formed. Conclusion MSCs could be induced to differentiate into cardiomyocyte-like cells by DMSO.
    Change of MMP-2/TIMP-2 after apoptosis of Hepatic Stellate Cell induced by FRNK overexpression
    Jian-gang SHEN; Xiao-lan ZHANG; Juan WEI; Xiao-xia HUO; Rong-xia SANG; Jun-yan AN
    2009, 29(9):  943-947. 
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    Objective To investigate the effect of breaking the phosphorylation of FAK by FRNK on apoptosis and the expression of MMP-2/TIMP-2 in HSC in vitro. Methods After FN stimulated HSC, FRNK plasmid mediated by cationic liposome was transfected into HSC. The apoptosis of FRNK-induced HSC was examined by Annexin-V/Propidium Iodide double-labeled flow cytometry (FCM), gel electrophoresis and transmission electron microscope. And the levels of FRNK, FAK, p-FAK (Tyr397), MMP-2 and TIMP-2 in HSC were assayed by Western blot on protein level, and by RT-PCR on mRNA level, respectively. Results The expression of FRNK was enhanced after FRNK has been transiently transfected into HSC in vitro. The apoptotic rate in HSC exposed to FRNK plasmid for 48 h was higher than that in the non-FRNK plasmid group(25.37%±1.92 % vs 9.28%±1.05 %), P<0.01]. After exposure of HSC to FRNK plasmid,compared with the non-FRNK plasmid group, the expression of TIMP-2 in protein and mRNA level reduced; On the contrary, the expression of MMP-2 increased. Conclusions FRNK can induce the HSC apoptosis and MMP-2/TIMP-2 was perhaps involved in the process.
    Expression of heat shock proteins in salivary tumor
    Gui-lan WANG; Xiao-lin GU; Li CHEN; Qun E; Bin CAO
    2009, 29(9):  948-954. 
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    Purposes The expression characteristics of heat shock proteins (HSPs) in salivary tumor were studied to find their effect and relationship on the occurrence and development of salivary tumor. Methods With immunohistochemistry techniques S-P,Immunofluorescence Double Staining Technique and Confocal Laser Scanning Microscope(CLSM),expression of HSPs was detected in 41 cases of benign tumors and 17 malignant tumors. The data were analyzed with stata7.0 chi square test and spearman correlation analysis. Results 1 All 5 HSPs were significantly expressed both in benign and malignant tumors. The expression rate of HSP84(β) and HSP86(α)was higher in benign mixed tumor(BMT)than in adenoma or adenolymphoma(p<0.01). 2 In malignant tumors,the expression of HSP27 was significantly lower(r=-0.3506, p<0.01),negatively related to malignant, while the expression of HSP70 or HSP86(90α)increased a little especially the double expression ratio of HSP70/HSP86(90α)reached 100%. Conclusions 1 All HSPs might be involved in tumorigenesis,but the types and expression rate were a little different. 2 The lower- expression of HSP27 might be the biomarker of differentiation depression in salivary tumor. 3 HSP70 and HSP90,especially HSP86(90α) may promote occurrence and development of malignant tumors through the persistence- activation of tumorigenesis client proteins. Hsp90,especially HSP86(90α),may be a target of salivary oncotherapy..
    Correlation between c-Jun activation domain binding protein 1 and p27kip1 in ovarian cancer cell HO-8910
    Wei-pei ZHU; Hong ZHANG; Zhi-hong QIAN; Li-fen LIU
    2009, 29(9):  955-960. 
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    Objective To investigate the expression and relationship of Jab1 and p27kip1 in ovarian cancer cell HO-8910. Methods Cell were treated with serum starvation and release. The expression and distribution of Jab1 and p27kip1 were detected by western blot and subcellular fractionation. HO-8910 cells were transfected in vitro with pcDNA3.1-myc-Jab1. Western blot as well as subcellular fractionation was used to detect the expression and localization of p27kip1. Results: The growth of HO-8910 cells was blocked by serum starvation. The amount of p27kip1 increased while the amount of Jab1 decreased. The reverse changes happened after serum release. p27kip1 and Jab1 could form compound in HO-8910 cells detected by immunoprecipitation. 48h after transfected by pcDNA3.1-myc-Jab1 , the expression of p27kip1 decreased and the distribution of p27kip1 translocated from nucleus into cytoplasma in HO-8910. Conclusion: Jab1 may influence the location and expression of p27kip1 through integrating with p27kip1, and then may participate in regulating the growth of ovarian cancer cells through interfering with the function of p27kip1.
    Ultrastructure changes of human unfertilized oocytes
    Zhi-xia TANG; Zhi-guo ZHANG; Qiong XING; Wei LIANG; Zhao-lian WEI; Yun-xia CAO
    2009, 29(9):  961-964. 
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    Objective To study the ultrastructure changes of human unfertilized oocytes and to investigate why human oocytes failed to fertilize after in vitro fertilization (IVF) /intracytoplasmic sperm injection (ICSI). Methods Gathered unfertilized oocytes after IVF/ICSI and normal matured oocytes were handled with fixed, dehydrated, embedded, ultrathined and observed by transmit electron microscopy(TEM) ,and to compare the difference of ultrastructure among the three groups .Results The thickness of zona pellucida of group IVF(14.7±2.4μm) was thicker than group normal (10.1±2.1)μm (P<0.05). In IVF group, cortical granules were dispersed in the oocytes cortex, only a part of which were beneath the oolemma. In other two groups, there was one row of dense cortical granules. The rate of abnormal mitochondria of group IVF (41.2%) was higher than other two groups (P<0.05). The oocyte cortex contained matured and immatured Golgi complex in group IVF, the presence of Golgi complex still forming cortical granules. Conclusions Organelles appeared in abnormal position was related to oocytes cytoplasmic maturation, which led to fail in fertilization. Failed fertilization in conventional IVF was due to absence of acrosome reaction and abnormal dense of the zona pellucida.
    RNAi Inhibits Survivin Expression and Increases Cell Apoptosis
    Fang XUE; Ming-ying DUAN; Yan CHEN
    2009, 29(9):  965-969. 
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    Objective Through interfering the expression of Surviving with short hairpin RNA (shRNA) technology, to investigate the effect of down regulation of SURVIVIN on cell and subsequent apoptosis in cervical cancer cells Hela. Methods 1.The U6 promoter was obtained by PCR from liver of mouse and ligated into TA vector. RNAi vector(psSURVIVIN) and psN(control vector) containing of the U6 promoter was Established. 2. Using Hela cells as a model system, two groups were set up stably transfected with RNAi control plamid and psSURVIVIN (SURVIVIN RNAi plasmid), respectively. The expression of SURVIVIN in Hela cells was measured by using western-blotting and immunofluorescence methods. 3. The activity of caspase 3/7 was detected using Caspase-Glo(tm) 3/7 Assay Reagent.Results 1.Cell apoptotic rate was significantly increased by transfection with RNAi targeting plasmid, and cell was arrested at G0/G1, (P<0.01) ; 2. SURVIVIN expression was significantly decreased by transfection with RNAi targeting plasmid; the expression proportion was reduced by nearly 70%.( P<0.01); 3.The activity of caspase 3/7 of psSURVIVIN group obviously increased ( P<0.01).Conclusion These results showed that by inducing RNAi-targeting plasmid, SURVIVIN expression could be effectively decreased,moreover,the activity of caspase 3/7 of psSURVIVIN group obviously increased.
    Influence of immunization procedure on antiserum titer of porcine plasma fibronectin
    Yan-qi LU; Hua GUO; Jian SHEN; Xue-jun WU; Zhang-cun WANG; Xiao-feng ZHOU; Jing-bo ZHANG
    2009, 29(9):  970-973. 
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    Objective To prepare Rabbit anti-pig plasma Fibronectin (FN) serum and to explore the effect of different immune approaches, the number of immunization and species of rabbits on the antibody titer. Methods Inbred New Zealand rabbits and cross bred rabbits respectively were inoculated by different programs which were hypodermic injection of 1 plus lymph nodethen injection of 5 or 3 times , intramuscular injection of 1 plus intravenous injection of 5 or 3 times with porcine plasma FN. Then, FN antibody was determined by the method of double immundiffusion. Results New Zealand rabbits and cross bred rabbits could bring anti-FN by hypodermic injection plus lymph nodethen injection or intramuscular injection plus intravenous injection. In addition, the antibody titer of the former was obviously higher than that of the latter and 5 times intensive immunization was higher than 3 times intensive immunization. Conclusions To obtain Rabbit anti-pig plasma FN serum, it may be better to inoculate rabbits through hypodermic injection plus lymph nodethen injection and multiple immunization.Moreover, inbred New Zealand rabbits were easy to induce anti-FN than cross bred rabbits .
    Thyroid hormone resistance with H435L mutation in exon 10 of thyroid hormone receptor gene associated with Hashimoto's thyroiditis
    Xiao-lan LIAN; Min NIE; Yao BAI
    2009, 29(9):  974-978. 
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    Objective To study mutations of thyroid hormone receptor β gene (TRβ) and clinical characteristics of a patient with resistance to thyroid hormone (RTH) associated with Hashimoto's thyroiditis (HT). Methods clinical manifestations were observed in a 7-year-old boy with RTH. Meantime, genomic DNA was extracted from peripheral blood of the patient and his parents. exons 5~10 of TRβ gene were amplified by PCR and sequenced directly. Results A heterozygote point mutation in exon 10 of TRβ was identified in the patient, which is a A to T transition in nucleotide 1403(c. 1304 A > T), resulting in His to Leu ( CAT→CTT) substituted at codon 435 ( H435L). No mutation was identified in the patient's parents. HT occurred in the patient during following-up. Conclusion H435L mutation in exon 10 of TRβ gene leads to resistance to thyroid hormone. Meanwhile, consistent RTH might lead to HT.
    Study for preparation and anticancer activity of the stealth epirubicin chitosan nanoparticles
    Li-wen GUO; Sen-ming WANG; Xi-gang HU; Man-ming CAO; Ji-ren ZHANG
    2009, 29(9):  979-983. 
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    Objective This study is to prepare a new type of chitosan nanoparticles (PEG/CS-EPI NPs), which contain epirubicin and modified by PEG, Furthermore, to investigate the anticancer activity of the NPs in vitro and in vivo. Methods The ionic gelation technique was employed to prepare the PEG/CS-EPI NPs and CS-EPI NPs. The particle size and morphology were determined respectively by laser scattering and transmission electron microscopy. The proliferation of nasopharyngeal carcinoma cells were detected by MTT assay in vitro; The mouse model of implantation murine sarcoma cells(S-180) were applied to evaluate the anticancer effectiveness of PEG/CS-EPI NPs and CS-EPI NPs in vivo. Results The PEG modified CS NPs prepared were discrete and uniform spheres with an average diameter of 322.1nm and the rates of drug loading and encapsulation is 13% and 74%, respectively. The results of the anticancer tests showed a sustained cytotoxicity of loading drug NPs on nasopharyngeal carcinoma cells in vitro and the stealth nanoparticles was more powerful than ordinary nanoparticles on the inhibitory potency in vivo. Conclution Stealth chitosan nanoparticles as compared with ordinary chitosan nanoparticles could be more suitable for the preparation of chemotherapy drug carriers.
    Diagnosis and treatment of renal oncocytoma and chromophobe cell renal carcinoma
    Hai-dong WANG; Ping ZHANG; Li-bo MAN; Yu-hai ZHANG
    2009, 29(9):  984-986. 
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    Objective To evaluate the clinical characteristic, diagnosis and treatment procedures for renal oncocytoma and chromophobe cell renal carcinoma. Methods 8 cases of renal oncocytoma and 5 cases of chromophobe cell renal carcinoma were analysed. The average of oncocytoma was 58.8 years old(23~74),and mean tumor size was 5.1 cm(range, 3~7cm); The mean age of chromophobe cell renal carcinoma was 54.5 years old(35~72),and mean tumor size was 5.8 cm(range, 3~8cm). Doppler ultrasonography and CT scan were performed in all patients. All cases accepted surgical operation and were confirmed by pathology. Results Renal oncocytoma was characterized by homogeneous round cell containing aboundant axyphil pellets. color Doppler ultrasonography mainly show isoechoic or hypoechoic masses .CT scan revealed that the tumors were homogeneous solid masses, and most of them were visualized as homogeneous enhancement.All the patients were followed up for 10 to 53 months,and no recurrence or metastasis noted. Chromophobe cell carcinoma composed of two cell types, typical and eosinophilic type, These cells were characterized by fine reticular cytoplasm and clear cell border. Color Doppler ultrasonography show mainly hyperechoic masses, CT scan revealed most of the tumors were heterogeneous enhancement.All the patients were followed up for 12 to 49 months,and 1 case died from metastasis after 1 year. Conclusion Renal oncocytoma and chromophobe cell renal carcinoma can be differentiated by clinical characteristic,ultrasonic, and CT scan. The final diagnosis depends on the pathology. Partial nephrectomy is good for renal oncocytoma, while radical nephrectomy is the first choice for chromophobe cell renal carcinoma.
    Study on the levels and correlation of glucose,lipids and inflammatory cytokines in MKR mice
    Wei HU; Rong YU; Xi-hua CHENG; Can-rong WU; Ping LI; Yu ZHAO; Jun-li LIU
    2009, 29(9):  987-988. 
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    CT-1 and JAKs immunolocalization in remodeling myocardium in physical training rats
    Yang WANG; Zhan-ling DONG; Zhi-fei LUO; Min-guang XU; Guo-bin CHEN; Shi-gan FU
    2009, 29(9):  989-991. 
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    Polymorphism of DNA repair gene XRCC1 in Chinese Han population
    Bao SONG; Jie LIU; Xian-rang SONG; Li XIE; Li-yan LV; Yan ZHENG
    2009, 29(9):  994-995. 
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    Application and development of genetic engineering technology in biopharmaceutics
    Li-xi ZHAO; Jing-jing SUN; Yong-ping JIANG
    2009, 29(9):  996-998. 
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    This article is focused on the perspective of genetic engineering technology in biopharmaceutical field. Four general aspects of genetic engineering are further discussed in this review as following: the separation of macromolecules, PCR, gene chip, and the expression of foreign genes. We also briefly review the large-scale or industrialization of genetic engineering technology in biopharmaceutics
    Progress in research on the role of heme oxygenase - 1 in cardiovascular diseases
    Ting-ting LI; Yuan GUO
    2009, 29(9):  999-1001. 
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    Heme oxygenase-1 (HO-1) is an inducible stress protein. In addition to its role in heme degradation, several positive biological effects exerted by this enzyme have gained attention, as anti-in?ammatory, anti-apoptotic, anti-proliferative and cytoprotective functions. This oxidase system with wide function takes part in the pathophysiology of cardiovascular system. It is related to multiple cardiovascular diseases including hypertension, atherosclerosis, ischemia /reperfusion injury and postangioplasty restenosis.
    Progress in the treatment of acute lung injury with Mesenchymal stem cells
    Li-kun ZHENG; Lei ZHANG; Nai-yao CHEN; Shou-ling WU; Hui ZHAO
    2009, 29(9):  1002-1006. 
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    Mesenchymal stem cell is a kind of multipotent hematopoietic stem cell. In the acute lung injury, it can differentiate into TypeⅠand TypeⅡepithelial cell, and repair impaired organization. In addition, mesenchymal stem cells have benefit effects in the treatment of lung injury by reducing proinflammatory factors IL-1,MIP-2,INF-γ, TNF-α, increasing antiinflammatory factors IL-10,IL-1ra, IL-13 and alleviating inflammatory response to the acute lung injury.
    Construction of Pathological Information Resources Storehouse for Teaching
    Ya-jun TAO
    2009, 29(9):  1007-1008. 
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    Based on the campus network platform and established the "pathological information resources for teaching" for specific applications in pathology teaching ,which can accumulate resources to strengthen pathology integration and the building of pathology courses to enhance the enthusiasm of the students, and promote the process of modernization of school education.