Basic & Clinical Medicine ›› 2009, Vol. 29 ›› Issue (9): 955-960.

• 研究论文 • Previous Articles     Next Articles

Correlation between c-Jun activation domain binding protein 1 and p27kip1 in ovarian cancer cell HO-8910

Wei-pei ZHU, Hong ZHANG, Zhi-hong QIAN, Li-fen LIU   

  1. the 2nd Hospital Affiliated to Suzhou University
  • Received:2008-04-03 Revised:2008-11-03 Online:2009-09-20 Published:2009-09-20

Abstract: Objective To investigate the expression and relationship of Jab1 and p27kip1 in ovarian cancer cell HO-8910. Methods Cell were treated with serum starvation and release. The expression and distribution of Jab1 and p27kip1 were detected by western blot and subcellular fractionation. HO-8910 cells were transfected in vitro with pcDNA3.1-myc-Jab1. Western blot as well as subcellular fractionation was used to detect the expression and localization of p27kip1. Results: The growth of HO-8910 cells was blocked by serum starvation. The amount of p27kip1 increased while the amount of Jab1 decreased. The reverse changes happened after serum release. p27kip1 and Jab1 could form compound in HO-8910 cells detected by immunoprecipitation. 48h after transfected by pcDNA3.1-myc-Jab1 , the expression of p27kip1 decreased and the distribution of p27kip1 translocated from nucleus into cytoplasma in HO-8910. Conclusion: Jab1 may influence the location and expression of p27kip1 through integrating with p27kip1, and then may participate in regulating the growth of ovarian cancer cells through interfering with the function of p27kip1.

Key words: Jab1, p27kip1, ovarian cancer