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Table of Content

    20 August 2009, Volume 29 Issue 8
    研究论文
    Enhancement of CEA specific RNAi on gene engineering adenovirus H101 treating tumor tissue of nude mice transplanted with human esophageal cancer cells
    Hong ZHENG; Guo-qiang ZHAO; Hong-yan YANG; Ji-min ZHAO; Yao-he WANG; Zi-ming DONG
    2009, 29(8):  785-788. 
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    Objective To study the effect of gene engineering adenovirus H101 treating esophagus carcinoma EC9706 induced by CEA gene specific silencing and to explore internal influential factor of H101 sensitivity. Methods To construct the siRNA expression vector of CEA and inhibit the expression of CEA through RNA interference and gene transfection in EC9706 cell, stable CEA gene silencing system were set up, compared with empty vector group and not transfected EC9706 cell, the model of athymic mouse subcutaneous transplantation tumor of human esophagus carcinoma EC9706 cell was established, injection in tumor was done with H101. The mRNA and protein expressions of CEA were detected by real time PCR and immunohistochemistry, the tumors'size were measured. Results Silencing CEA gene by applying RNAi can inhibit CEA mRNA and protein expression in nude mice model with transplanted human esophageal cancer cells, there was no evident influence on speed and volume of forming tumor. After using H101, the tumor size of interfering group was much less than empty vector group and normal control group, (P<0.05).Conclusion Inhibiting CEA expression in esophagus carcinoma EC9706 cell, the sensitivity of H101 can enhance .
    Expression of NKG2D ligands could be selectively induced by oxidative stress
    Cheng-hong LI; Lian-xian CUI; Wei HE; Chi MA
    2009, 29(8):  789-795. 
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    Objective To study the relationship between the expression of NKG2D ligands and oxidative stress,and analyze the effect of oxidative stress on the function of NK cells. Methods Tumor cells were cultured and exposed to hydrogen peroxide to make an oxidative stress model. Then we detected the expression of NKG2D ligands in cells by Real-time PCR and Flow Cytometry. The cytotoxicity of NK cells to tumor cells was detected and compared by CCK-8 kit before and after oxidative stress. Results The expression of NKG2D ligands could be induced by oxidative stress,however the NKG2D ligands induced was variable. The up-regulation of NKG2D ligands increased the cytotoxicity of NK cells efficiently, and this effect could be blocked by anti-NKG2D antibody. Conclusion The expression of NKG2D ligands could be selectively induced by oxidative stress on tumor cells, and the improvement of the cytotoxicity of NK cells may enhance the immune responses accordingly.
    Clinical analysis of Dentinogenesis imperfecta type Ⅱ in a large Mongolian family
    Qi-zhu WU; Hai-hua BAI; Xin-yuan ZHANG; Yu-jie CHEN; Hai-ping LIU; Chang-chun QIU
    2009, 29(8):  796-800. 
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    Objective To decipher the clinical characteristics and genetic bases of Dentinogenesis imperfecta type II in a large Mongolian family. Methods Systematic analysis towards this family was carried out using clinical detection. Results Affected individuals of Dentinogenesis imperfecta type II were consecutively found in a five-generation family. The morbidity of the offsprings is nearly 1/2 and no sexual difference is observed. The analysis of clinical features as well as dental x-ray film show that it is not completely the same compared with other families. Conclusions Dentinogenesis imperfecta type II in this Mongolian family pertains to autosomal dominant disorder with high genetic heterogeneity in clinical phenotype. Further study is warranted to investigate the association of this heterogeneity with lifestyle or genetic information.
    Co-expression of multiple drug resistance gene protein 1 and glial fibrillary acidic protein in patients with temporal lobe epilepsy in temporal lobe and hippocampus
    Zhi-jun YANG; Chun-zhi WANG; Rao WANG; Hong-liu LU; Ru-xiang XU; An-min LI; Shu-li LIANG
    2009, 29(8):  801-805. 
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    Objective To detect the expression of multiple drug resistance gene protein 1(MDR-1), neuron-specific endase (NSE), and glial fibrillary acidic protein (GFAP) in the temporal lobe and hippocampal dentate gyrus of patients with temporal lobe epilepsy.Methods In epileptic group, the brain tissues including dentate gyrus and temporal lobe were obtained by operation in 12 patients with temporal lobe epilepsy. In control group, the brain tissues were obtained from 4 dead patients underwent autopsy. By means of anti-MDR1 and GFAP or NSE double immunofluorescence histochemical staining method combining with confocal laser-scanning microscopic technique, the distribution of MDR1 or NSE positive cells and reactive astrocytes were observed in the temporal lobes and hippocampus.Results In control group, many GFAP positive astrocytes and NSE positive neuron could be found in the cortex of temporal lobe and dentate gyrus of hippocampus. No MDR1 positive cells could be observed in above-mentioned areas.In epileptic group,GFAP positive astrocytes within the cortex of temporal lobe and dentate gyrus of hippocampus increased markedly. GFAP and MDR1 could co-express in the same astrocyte in the cortex of temporal lobe and dentate gyrus of hippocampus. Conclusion It was suggested that multidrug resistance gene of astrocytes might play an important role in the pathogenesis of intractable epilepsy.
    The detection of the nucleation shifting process of Smad2 signal protein in Rat dental papillae cells with Quantum Dots-Smad2 monoclonal antibody Fluorescent probes
    Rui CHEN; Kai YANG; De-ping SUN; Guo-dong ZHANG; Jie MEI; Ya-dong LI
    2009, 29(8):  806-810. 
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    Objective To prepare Semiconductor Quantum Dots (QDs)-Smad2 monoclonal antibody Fluorescent probes , to detect nucleation shifting process of Smad2 signal protein in Rat dental papillae cells(RDPCs)stimulated by TGF-β1 with the Fluorescent probes.Methods ① QDs were chemically modified with Smad2 proteins to prepare water soluble QDs-Smad2 monoclonal antibody Fluorescent probes which were purified after the preparation, and detect its related optical properties. ②the location of Smad2 proteins in RDPCs was studied with QDs direct mark method、Smad2 monoclonal antibody direct mark method and direct immunofluorescence imaging. And the properties of Quantum Dots-Smad2 monoclonal antibody Fluorescent probes in RDPCs were detected. ③after RDPCs cultivated by TGF-β1 for 0 hours、12 hours、24 hours ,the dynamic nucleation shifting process of Smad2 signal protein in RDPCs was separately detected with direct immunofluorescence imaging.Result QDs and monoclonal antibody covalently bond to form the Fluorescent probes which could specifically and effectively recognize Smad2 proteins in RDPCs .It is to demonstrate the dynamic nucleation shifting process of Smad2 signal protein in RDPCs. These Fluorescent probes still had good high fluorescence intensity and photostability. conclusion QDs and monoclonal antibody could covalently bond to form the Fluorescent probes with distinct optics character、special immunity recognition capability and the ability of imaging mark the protein in cells for long time .
    Polymorphisms within GNB3, ADD1 and ADRB2 genes are not associated with HAPE in China
    Yue QI; Jing-liang LIU; Ying XU; Wei-ya DONG; Shou-quan DING; Guo-shu YU; Tong-chun ZHU; Chang-chun QIU
    2009, 29(8):  811-815. 
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    Objectives: We aimed to explore the genetic susceptibility of three polymorphisms within GNB3、ADD1 and ADRB2 genes to high altitude pulmonary edema (HAPE). Methods: The rs5443 in GNB3 gene, rs4961 in ADD1 gene and rs1042713 in ADRB2 gene were genotyped by polymerase chain reaction-restriction fragment length polymorphism, as well as allelic specific polymerase chain reaction. Results: No significant frequency differences were found in terms of any of the three polymorphisms between the HAPE and control groups. Meanwhile, no relationships were observed between the haplotypes of the three polymorphisms and HAPE. Conclusion: All of the studied polymorphisms within GNB3、ADD1、ADRB2 genes might not be involved in the pathogenesis of HAPE.
    Effect of rosiglitazone on no-reflow after reperfusion in rats with AMI
    Quan HE; Han LEI; Qing LIU; Shu QIN; Kang-hua MA; Xi WANG
    2009, 29(8):  816-820. 
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    Objective To investigate the effect of rosiglitazone on no-reflow and eNOS expression after reperfusion in rats with AMI. Methods Left anterior descending coronary artery was undergone 3 h of occlusion and 2 h of reperfusion to establish the model of no-reflow. 60 SD rats were randomly divided into the control group、the sham operated group、the rosiglitazone group、rosiglitazone plus L-NNA group、L-NNA group; thioflavin-S and Evan's blue were used to delineate the ligation area (LA) and area of no-reflow (ANR); Western blot for phosphorylated eNOS at Ser1177、RT-PCR for mRNA expression of eNOS were performed. Results (1)Compared with control group, area of no-reflow in rosiglitazone group were significantly reduced(P<0.05), but not in rosiglitazone plus L-NNA group; (2)Compared with the control group,there was no significant differences in mRNA expression of eNOS in rosiglitazone group, whereas Level of p-eNOS Ser1177 protein was significantly increased (P<0.05). Conclusions Rosiglitazone significantly reduced area of no-reflow after reperfusion in rats with AMI, the mechanism of which may be partly due to its role in improving phosphorylation of eNOS, and further increased production of NO.
    valsartan inhibits the expression of connective tissue growth factor in rat glomerular mesangial cells under high concentration glucose
    Li-hui WANG; Guang-li WU; Li-xia ZHANG; Xu-dong HUANG; Sai LI
    2009, 29(8):  821-826. 
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    Obiective To investigate the effects of valsartan on the expression of connective tissue growth factor(CTGF) in rat glomerular mesangial cells under high concentration of glucose .Methods we used high concentration glucose and valsartan to stimulate the cultured rat glomerular mesangial cells in vitro. The protein expressions of CTGF and the activation of p38 mitogen-activated protein kinase(p38 MAPK) and cAMP response element-bingding protein(CREB1) ) were observed by Western blot. CTGF and fibronectin(FN) mRNA were measured by reverse transcription and polymerase chain reaction (RT- PCR). The protein synthesis of lamanin (LN) and type IV collagen in the supernatants of the GMCs were detected by radioimmunoassay. Results Compared with low glucose control group, the expression of CTGF,p-p38 MAPK, p-CREB1,CTGF mRNA, FN mRNA, LN and type IV collagen in the supernatants were significantly increased in GMCs under high concentration of glucose medium. The expression levels of CTGF,p-p38 MAPK,p-CREB1 ,CTGF mRNA and FN mRNA were significantly lower in the valsartan group than those in the high concentration glucose group. The concentrations of LN and type IV collagen in the supernatantsin the valsartan group were also lower than those in the hiagh concentration glucose group. Conclusion  Valsartan can inhibit expression of CTGF and ECM proteins in rat glomerular mesangial cells under high concentration of glucose, partly by regulating the phosphorylation of P38 MAPK and CREB1 .
    5-azacytidine actives three types of K+ currents during MSCs proliferating and differentiating processes
    Ou-yang CHEN; Geng-sheng YU; Jie TIAN; Heng-sheng CHEN; Yuan CHEN; Yong-ru QIAN
    2009, 29(8):  827-830. 
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    Objective To study the changes of the transient outward K+ current (Ito), delayed rectifier K+ current(IkDR) and the inward rectifier K+ current(Ik1) of the rat bone mesenchymal stem cells (MSCs) induced by 5-azacytidine(5-Aza) during proliferating and differentiating process in vitro. Methods The MSCs were cultured according to related articles for two weeks and then some of the cells were induced by 5-azacytidine .The experiment was divined into uninduced(cultured for 6w) and induced(1、2、3、4w) cell groups. Each week had twenty cells random tested by the whole-cell patch clamp technology and the K+ currents were identified by corresponding ionic blockers. Results The detection rate of the same type of K+ current among test weeks had no significant deviation; The three types of K+ current intensity were gradually augmented after being inducing cultured for 1﹑2﹑3﹑4weeks(p<0.05).When compared to the uninduced cells, the same type of K+ current intensity of 1w-induced cells had no significant deviation, however, the current intensity was augmented begin at 2w-induced cells(p<0.05)and significant augmented at 4w-induced cells(p<0.01).Conclusion 5-Aza induction had the early stage augmented effects to the MSCs induced by 5-Aza during proliferating and differentiating process in vitro.
    Interactions of Helicobacter pylori and its' L-forms with surfactant protein D
    Zheng-hong CHEN; Liang ZHAO; Fei WANG
    2009, 29(8):  831-834. 
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    Objective To explore the ability of combination of Helicobacter pylori (Hp) and its' L-form with Surfactant protein D(SP-D), and the function of SP-D in Hp infection and immunity. Methods SP-D were extracted from the lung of rats by Tris-HCl-EDTA and maltose-agarose affinity column. The combination of Hp and SP-D were detected by slide and tube agglutination tests, and the agglutination inhibition with SP-D immune sera was carried out. The L-form of Hp was induced by Ceftriaxon sodrum and identified by PCR and sequencing of the 16SrDNA fragment.The combination of SP-D and the L-form was detected by the slide agglutination test. Results Agglutination of Hp NCTC11637 and SP-D could be observed on slide, while which of NCTC11639, SS1 and the L-form couldn't. Agglutination could be detected 45 minutes after reaction and could be inhibited by SP-D immune sera. Conclusions The ability of combination with SP-D could be different between strains of Hp.The combination could be weakened or vanished when Hp lost the cell wall.
    Expression of signal transduction pathway genes in ARDS transcription profiles of rats
    Xiu-zhen LIU; Feng LIU; Ru-yi ZHANG; Xiu-li ZHANG; Xiao-hua ZHANG; Hai-yun LUAN
    2009, 29(8):  835-839. 
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    Objective This study aims to study the signal transduction pathway genes in ARDS(Acute respiratory distress syndrome ) of rats by genechip. Methods The total RNAs were isolated from lungs of normal and ARDS rats respectively. The RNAs were purified by oligotex. Both the mRNAs from two kinds of tissues were reversely transcribed to cDNA with the incorporation of fluorescent-labelled dUTP to prepare the hybridization probes. The mixed probes were hybridized to cDNA microarray. Picture signals of fluorescence in gene array were scanned and compared by CapitlBio Molecule Annotation System V4.0. Quantitative fluorescence RT-PCR was used to validate the results of genechip. Results The results showed that there were 2 genes up-regulated and 9 genes down-regulated over 1.5 times in lung tissues of ARDS rats,and these genes were involved in 11 signal transduction pathways. Conclusion: Many genes of signal transduction pathways expressed differentially in ARDS transcription profiles of rats.
    Biocompatibility of Chitosan-Mg membranes with PC12 cells in vitro
    Chuang-lai ZHU; Hui-fang LI; Lin LIN; Tian-yi ZHANG
    2009, 29(8):  840-844. 
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    Objective To prepare the chitosan-Mg membranes and explore the biocompatibility of membranes with PC12 cells and feasibility of its usage as biomaterials in tissue engineering. Methods The appearance of the chitosan-Mg membranes (CM) was observed under scanning electromicroscope (SEM) and the element of the membranes was analyzed by x-ray energy spectromete. PC12 cells were co-cultured with CM in vitro, the morphological changes of PC12 cells on membranes were observed under scanning electron microscope (SEM) and light microscope (LM), and the cell vitality was detected by MTT assay. Results: The surface of chitosan membranes (CS) was smoother than that of CM;but the CM groups were full of pore observed under SEM;The content of Mg element was related with the dose of MgSO4 added into chitosan solution in dosage dependence. Morphology observation showed that PC12 cells grew well on CM compared with CS group. The cells were rich of microvilli and long processes on 7th day, and synapses alike structure was formed in many PC12 cells. In addition, the cell viability of experiment group was higher than that of control group(P<0.05)after 5 days detected by MTT method. Conclusion Chitosan-Mg membrances has good biocompatibility with PC12 cells.
    Oxytocin inhibited peripheral stimulation-induced LTP and Fos protein expression in hippocampus of rats
    Dan SHU; Jiang WU; Shou-qin SHANG GUAN; Qi-sheng HU
    2009, 29(8):  845-849. 
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    Objective To investigate the effects of Oxytocin(OT) on hippocampal long-term potentiation(LTP) and Fos protein expression which induced by peripheral stimulation. Methods Single stimulation pulses were delivered to the left sciatic nerves to evoke the field excitatory postsynaptic potentials (fEPSPs) in the right hippocampal CA1. Tetanic stimulation were used to induce hippocampal LTP. Different groups of rats were given NS, OT, Oxytocin antagonist- Atosiban + OT before tetanic stimulation, into lateral ventricle(LV) respectively. Expression of Fos protein were compared among the groups by histopathological and immunohistochemistry. Results Single stimulation evoked fEPSPs in hippocampal CA1, which average latency was 171.9±33.1ms and average amplitude was 25.7±8.4μV. Tetanic stimulation induced hippocampal LTP and increased the expression of Fos protein. Intracerebroventricular injection of OT inhibited hippocampal LTP and decreased the expression of Fos protein. This effect of OT was blocked by the pretreatment with Atosiban. Conclusions The results suggested that OT may play an inhibitory role in leaning and memory of rats; the effect is mediated by OT receptor.
    OPN antisense oligodeoxynucleotide inhibits proliferation and apoptosis of human breast carcinoma cell line
    Yun-song ZHOU; Xiao-hui WEN; Lin-xi YANG; Peng CHEN
    2009, 29(8):  850-854. 
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    Objective To investigate the effects on anti-expression of Osteopontin (OPN) by an antisense oligodeoxynucleotide (ASODN) targeting to low free energy region of OPN mRNA and its effects on proliferation and apoptosis of human breast carcinoma cell line MCF-7. Methods Designing and synthesizing an ASODN based on minimum free energy algorithm in vitro, which targets to the low free energy region of OPN mRNA. Transfecting it into breast carcinoma cell line MCF-7 which expresses OPN in high level. The cell proliferation-inhibitory rate was determined by MTT method; The morphologic changes was observed by transmission electron microscope (TEM); The OPN mRNA expression level was checked by RT-PCR method; Cell cycle and apoptosis rate were detected by flow cytometry (FCM) after transfection ,respectively. Results OPN ASODN could inhibit the proliferation of the cells in both time and concentration dependent manner (P<0.05).Characteristic morphologic apoptosis changes was observed after incubated with 16?mol/L OPN ASODN for 72h. OPN mRNA expression in ASODN groups were notably decreased. there was a significant high apoptosis rate in ASODN groups (P<0.01). Conclusions OPN ASODN transfection significantly inhibits the proliferation of human breast carcinoma MCF-7 cells and induces cell apoptosis in vitro. It's a reliable method to design ASODN based on minimum free energy algorithm.
    Effects of progesterone on survivin in ishikawa endometrial carcinoma cell line
    Su-min QIAN; Hui-lan WANG
    2009, 29(8):  855-858. 
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    Objective To investigate the effects of progesterone on apoptosis and surviving expression protein of Ishikawa cell line of human endometrial carcinoma in vitro, Methods The proliferational of Ishikawa cells cultured in vitro were determined by methyl thiazolyl terazolium (MTT) medhod.Cell cycle, apoptotic percentage were detected by cytometer . The expressions of survivin protein of Ishikawa cells were detected by immunocytochemistry. Results progesterone inhibited the growth of Ishikawa cells significantly when the concentration of progesterone was at 0.5×10-5~10-4mol/l(p<0.01). Flow cytometry(FCM) showed that the proportion percentage of G0/G1 phase and the apoptotic rate were increased significantly. The proportion of S phase was reduced (p<0.01). Apoptosis morphology was observed in each group . The expression of survivin of in Ishikawa cells was lowed after the cells was treated with P. Conclusion Progesterone could inhibit the proliferation and induce apoptosis of Ishikawa cells. The regulation on the expression of the survivin protein by progesterone seems to be responsible for the effects on the apoptosis in Ishikawa cells.
    技术与方法
    Intracellular labeling of bone marrow stromal cells of Crab-eating Macaque with Feridex
    Zhi-jun YANG; Yong-chun LUO; Ru-xiang XU; Yi-quan KE; Xiao-dan JIANG; Ying-qian CAI; Mou-xuan DU; Yu-xi ZOU
    2009, 29(8):  859-862. 
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    Objective: To explore the feasibility of protocols using Feridex and transfection agents for in vitro magnetic labeling of bone marrow stromal cells (BMSCs). Methods: Under the sterile condition, the crab-eating Macaque bone marrow stromal cells were harvested by means of density gradient centrifugation following a bone puncture. Feridex-poly-l-lysine (FE-PLL) complexes were used to magnetically label BMSCs. The efficiency and cellular viability of FE-PLL labeled BMSCs were evaluated by Prussian blue staining, electron microscopy, and trypan blue dye exclusion test. The proliferation and differentiation ability of FE-PLL labeling BMSCs were also investigated by Inverted phase contrast microscope and immunocytochemistry. Results: BMSCs could be effectively labeled and labeling efficiency were around 99%. Numerous blue stained fine particles and numerous vesicles filled with the electron-dense magnetic iron particles could be found in the cytoplasm of FE-PLL labeled BMSCs under optical microscopy and transmission electron microscopy respectively. Cell viability, proliferation and differentiation ability of labeled cell were not affected by endosomal incorporation of Feridex nanoparticles. Conclusion: The Feridex might be used to label BMSCs of crab-eating Macaque.
    Construction of non-fusion recombinant of pAAV-TK-IRES-ES and primary identify of its function
    Jian-gang PAN; Xing ZHOU; Rui-fa HAN; Zhi-guang CHEN; Jiang-ting LI
    2009, 29(8):  863-866. 
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    Objective To construct pAAV-TK-IRES-ES and identify its function. Methods①IRES,TK,ES framents from pIRES-MCS, pAAV-TK, pAAV-ES were attained by PCR and then cloned into vector pMD-19T simple to construct pAAV-TK-IRES-ES.②Viral particle of purified rAAV were assayed by AVSachTM ELISA.③Identify the primary function of r AAV-TK-IRES-ES via T24 cell and HUVEC cell Result ①pAAV-TK-IRES-ES was constructed and tested correctly by sequence indentification and enzyme digestion.②We obtained high quality of rAAV after dissociating and purifying. The viral particles title of rAAV were 2×1011-12 v.p/ml. ③ rAAV-TK-IRES-ES has both functions of endostatin and suicide gene by inducing cell apoptosis of T24 and HUVEC cell. Conclusion We successfully constructed rAAV-TK-IRES-ES and it can inhibit tumor induced angiogenesis and suppress both the initiation and the subsequent growth of human bladder cancer.
    临床园地
    Intermittent infusions of ibandronate for treatment of Beijing postmenopausal osteoporotic women
    Mei LI; Xiao-ping XING; Wei-bo XIA; Ying-ying HU; Ou WANG; Yan JIANG; Huai-cheng LIU; Xun-wu MENG
    2009, 29(8):  867-871. 
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    Objective To assess the efficacy and tolerance of infusions of ibandronate on Beijing postmenopausal osteoporotic women. Methods A randomized study among 60 postmenopausal women receiving either 2-hour infusions of ibandronate(2mg) in every three months or oral 70mg/week alendronate during one year. The bone mineral density (BMD, by DXA), serum carboxy-telopeptide cross-links of type I collagen(CTX, by chemiluminescence) and alkaline phosphatase (ALP,by autonomic analysor) were measured to evaluate the efficacy. The side effects, liver and kidney function were observed to assess the tolerability. Results: 59 patients completed the observation. After one year of treatment, at the sites of lumbar spine, femoral neck and trochanter, ibandronate increased the BMD by 6.3%, 2.5% and 0.1% (lumbar P <0.001,femoral neck P <0.01), and alendronate changed the BMD by 3.7%, 4.9%, -0.5% respectively(lumbar and femoral neck, P <0.001). There were no significant differences of BMD between two groups after treatment. In ibandronate and alendronate group, ALP levels were decreased by 15.8% and 17.2%,CTX levels decline by 78.1% and 43.2%( P <0.001). The liver and kidney function were in normal range. Mild muscle pain and fever in the first month after infusion (26.7%) was the main side effects of ibandronate. Mild upper GI effect(13.3%) was the common effect of alendronate. Conclusions Intermittent infusions of ibandronate significantly increased BMD and inhibited bone resorption with good tolerance in postmenopausal osteoporotic women.
    DMD gene defection analysis of 135 patients of duchenne muscular dystrophy
    Yang YAN; Xiao-feng YANG; Fu-hua YIN; Sheng-nan HUANG
    2009, 29(8):  872-874. 
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    objective:deletion detection of Duchenne muscular dystrophy(DMD);method: the 135 patients use polymerase reaction of amplification with 12 dystrophin exons, polyacrylamide gel electrophoresis make gene analysis; result:The deletion of different region was found in 54 patients; conclusion: the deletion region is concentrated in 45-53exons,the deletion of 48 exons is maxium.
    Quick response to thiamazole treatment of a patient with Graves' disease
    An-li TONG; Yu-xiu LI; Xiao-lan LIAN; Ling-ling XU
    2009, 29(8):  875-877. 
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    研究短文
    Sodium tanshinone Ⅱ-A sulfonate inhibits expressions of PCNA and Cyclin E in human renal interstitial fibroblasts in vitro
    Guo-hua YU; Ling-ling KONG; Fei HUANG; Xiang-mei KONG; Gui-mei QU; Wei-dong YAO; Xing-wang SUN
    2009, 29(8):  878-879. 
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    Inhibition of telmisartan on microcirculation disturbance induced by rabbits' myocardial ischemia/reperfusion in vivo
    Xiao-cong ZENG; Xing-san LI; Hong WEN
    2009, 29(8):  880-882. 
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    Dinitrophenyl-modified tumor vaccines enhance PBMCs' cytotoxicity against human breast cancer cells in vitro
    Wei WANG; Yan HU; Zhao SUN; Jin-hong DUAN; Shu-chang CHEN; Xiao-ming WANG; Xian-da YANG
    2009, 29(8):  883-884. 
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    短篇综述
    Recent advances in the pancreatic cancer stem cell research
    Lei YOU; Ge CHEN; Yu-pei ZHAO
    2009, 29(8):  885-887. 
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    Only a specifc subset of cancer cells within each tumor which have a character of self-renewal and multilineage differentiation, and is responsible for tumor initiation ,propagation and chemoresistance, termed cancer initiating cells or cancer stem cells (CSCs). This review summarizes the latest advances about the isolation and identification of pancreatic cancer stem cells and and its relationship with invasion and metastasis, chemotherapy resistance.
    Clinicopathologic characteristics ,diagnosis and treatment of rectal gastrointestinal stromal tumor
    Bao-hua WANG; Guan-nan ZHANG; Yi XIAO; Hui-zhong QIU
    2009, 29(8):  888-891. 
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    The incidence of rectal gastrointestinal stromal tumor is low,adequate diaganosis depends on the histopathological and immunohistochemical examination.Its treatment is a comprehensive therapy including surgery and molecular targeted therapy etal.The rectal gastrointestinal stromal is easily recurrent after operation,and needs surveillance and following up.