基础医学与临床 ›› 2024, Vol. 44 ›› Issue (2): 174-179.doi: 10.16352/j.issn.1001-6325.2024.02.0174

• 研究论文 • 上一篇    下一篇

粉防己碱减轻IL-1β诱导的体外人关节软骨细胞损伤

欧阳云朗, 张宁, 陈丽萍, 贾丙申, 焦拓*   

  1. 海南医学院第一附属医院 关节外科, 海南 海口 570000
  • 收稿日期:2023-01-10 修回日期:2023-10-09 发布日期:2024-02-05
  • 通讯作者: *397753044@qq.com
  • 基金资助:
    海南省卫生健康行业科研项目(20A200025)

Tetrandrine attenuates IL-1β-induced injury of human articular chondrocytes in vitro

OUYANG Yunlang, ZHANG Ning, CHEN Liping, JIA Bingshen, JIAO Tuo*   

  1. Department of Joint Surgery, the First Affiliated Hospital of Hainan Medical College, Haikou 570000, China
  • Received:2023-01-10 Revised:2023-10-09 Published:2024-02-05
  • Contact: *397753044@qq.com

摘要: 目的 粉防己碱(Tet)对白细胞介素1β(IL-1β)诱导原代人关节软骨细胞损伤的影响。方法 将人关节软骨细胞分为对照组、IL-1β组、低氧诱导因子-1α(HIF-1α)抑制剂[二甲氧基雌二醇(2-ME2)]组、Tet低、中、高浓度组,每组设置6个重复。MTT法检测细胞增殖;流式细胞仪检测细胞凋亡;ELISA检测细胞中炎性相关因子肿瘤坏死因子-α(TNF-α)、基质金属蛋白酶3(MMP-3)、诱导性一氧化氮合酶(iNOS)、环氧合酶-2(COX-2)的水平以及抗氧化因子超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPx)的活性;Western blot检测细胞中HIF-1α、VEGF蛋白的表达。结果 与对照组相比,IL-1β组细胞的凋亡率、TNF-α、MMP-3、iNOS、COX-2的水平以及HIF-1α、VEGF蛋白表达均升高,细胞存活率、SOD和GPx活性均降低,差异有统计学意义(P<0.05);与IL-1β组相比,2-ME2组和Tet低、中、高浓度组细胞的凋亡率、TNF-α、MMP-3、iNOS、COX-2的水平以及HIF-1α、VEGF蛋白表达均降低,细胞存活率、SOD和GPx活性均升高,差异有统计学意义(P<0.05)。结论 粉防己碱减轻IL-1β诱导的人关节软骨细胞损伤。

关键词: 粉防己碱, 骨关节炎, 白细胞介素1β, 关节软骨细胞, 损伤

Abstract: Objective To investigate the effect of tetrandrine (Tet) on injury of primary human articular chondrocytes induced by interleukin-1β (IL-1β). Methods Human articular chondrocytes were divided into control group, IL-1β group, hypoxia inducible factor(HIF-1α) inhibitor group [2-methoxyestradiol(2-ME2) group], Tet groups containing low, medium and high concentrations. Each group has six replicated samples. MTT assay was used to detect the viability of cells; cell apoptosis was detected by flow cytometry; the level of inflammatory related factors like tumor necrosis factor-α (TNF-α), matrix metalloproteinase 3(MMP-3), inducible nitric oxide synthase (iNOS), cyclooxygenase-2(COX-2) and the activity of antioxidant factors like super oxide dismutase (SOD) and glutathione peroxides (GPx) in cells were detected by ELISA; Western blot was used to detect the expression of HIF-1α and VEGF in cells. Results Compared with the control group, the apoptosis rate, level of TNF-α,MMP-3, iNOS,COX-2 and the protein expression of HIF-1α and VEGF in IL-1β group all increased, the cell survival rate and the activity of SOD and GPx significantly decreased (P<0.05); compared with IL-1β group, the apoptosis rate, the level of TNF-α, MMP-3, iNOS, COX-2 and the protein expression of HIF-1α and VEGF in 2-ME2 group and Tet groups with low, medium, and high concentration significanthy decreased(P<0.05). The cell survival rate and the activity of SOD and GPx significantly increased(P<0.05). Conclusions Tetrandrine attenuates IL-1β-induced injury of human articular chondrocytes.

Key words: tetrandrine, osteoarthritis, interleukin-1β, articular chondrocytes, damage

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