趋化因子CX3CL1具有抗小鼠肝纤维化的潜在作用

doi:10.16352/j.issn.1001-6325.2023.12.1792

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (12): 1792-1795.doi: 10.16352/j.issn.1001-6325.2023.12.1792

趋化因子CX3CL1具有抗小鼠肝纤维化的潜在作用

程琦, 李宁, 鱼康康, 施光峰^*

复旦大学附属华山医院感染科,上海 200040

收稿日期:2023-04-13 修回日期:2023-07-18 出版日期:2023-12-05 发布日期:2023-11-29
通讯作者: ^* gfshi@shmu.edu.cn
基金资助:
国家自然科学基金(81770565)

Chemokine CX3CL1 has potential anti-fibrosis effect in mouse liver fibrosis

CHENG Qi, LI Ning, YU Kangkang, SHI Guangfeng^*

Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China

Received:2023-04-13 Revised:2023-07-18 Online:2023-12-05 Published:2023-11-29
Contact: ^* gfshi@shmu.edu.cn

摘要/Abstract

摘要： 目的探讨趋化因子CX3CL1在小鼠肝纤维化模型中的作用及其机制。方法将实验小鼠随机分成CCl4造模组和正常对照组,每组8只。造模方法为6周持续腹腔内注射CCl4。6周后,观察肝脏组织的病理变化,并检测小鼠外周血CX3CL1水平,以及肝组织CX3CL1 mRNA及蛋白的表达水平。为探寻CX3CL1发生作用的机制,检测小鼠血清中IFN-γ水平。结果造模6周后,肝脏病理显示肝纤维化模型小鼠成功建立,并且模型组血清CX3CL1水平及肝脏组织 CX3CL1表达明显上调。另外模型组小鼠血清中IFN-γ明显上升。结论 CX3CL1与小鼠的肝纤维具有相关性,其机制可能促进IFN-γ的产生,发挥抗肝纤维化作用。

关键词: 趋化因子, CX3CL1, 肝纤维化, 干扰素-γ

Abstract: Objective To explore the effect and mechanism of chemokine CX3CL1 on mouse liver fibrosis model. Methods C57BL/6 mice were randomly divided into CCl-4 model group and normal control group with 8 animals in each group. The model group was injected with 10% CCl-4 intra peritoneally for 6 weeks. After 6 weeks, the mice were sacrificed, and the pathological changes of the mouse liver were observed by HE staining. The level of CX3CL1 in peripheral blood of the mice was measured, and the expression level of CX3CL1 mRNA in the liver tissue of the mice was detected. In addition, in order to explore the mechanism of CX3CL1, the level of IFN-γ in mouse serum was detected as well. Results After the 6-week modeling, the liver pathology microscopy showed a successful modeling of liver fibrosis. The serum CX3CL1 level and liver tissue CX3CL1 expression in the model group were found to be significantly up-regulated, which suggested a potential impact on the pathogenesis of liver fibrosis. In addition, the level of IFN-γ in the serum of mice in the model group increased significantly. Conclusions CX3CL1 is related to liver fibrosis in mice, and its mechanism might be explained by promoting the production of IFN-γ so to prevent liver fibrosis.

Key words: chemokine, CX3CL1, liver fibrosis, interferon-γ

中图分类号:

R575

程琦, 李宁, 鱼康康, 施光峰. 趋化因子CX3CL1具有抗小鼠肝纤维化的潜在作用[J]. 基础医学与临床, 2023, 43(12): 1792-1795.

CHENG Qi, LI Ning, YU Kangkang, SHI Guangfeng. Chemokine CX3CL1 has potential anti-fibrosis effect in mouse liver fibrosis[J]. Basic & Clinical Medicine, 2023, 43(12): 1792-1795.

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趋化因子CX3CL1具有抗小鼠肝纤维化的潜在作用

Chemokine CX3CL1 has potential anti-fibrosis effect in mouse liver fibrosis

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