关键词: 寻常型银屑病, CXC趋化因子配体8, miR-150, 病情严重程度

Abstract: Objective To investigate the relationship between the expression of CXC chemokine ligand 8 (CXCL8) and miR-150 in the skin lesions of patients with psoriasis vulgaris and the severity of the disease. Methods The skin lesions of 80 patients with psoriasis vulgaris who were treated in Nanyang first people's Hospital from January 2020 to May 2021 were selected as the research objects. The normal tissues around the skin lesion were chosen as a control. The Psoriasis Area and Severity Index (PASI) was used to classify patients with psoriasis, the immunohistochemical method was used to detect the expression of CXCL8 protein, the real time fluorescence quantitative PCR method was used to detect the expression of miR-150, Western blot was used to detect the expression of CXCL8 protein, and the correlation between the expression levels of CXCL8 protein, miR-150 and the severity of the disease was further analyzed. Results The level of CXCL8 mRNA in the skin lesion of patients with psoriasis vulgaris was higher than that in normal tissues (P＜0.05) and the level of miR-150 was lower than that in normal tissues(P＜0.05). The expression of CXCL8 protein in the skin lesions of patients was 68.75%, which was significantly higher than 37.50% in normal tissues (P＜0.05); the expression of CXCL8 protein in the skin lesions of patients was significantly higher than that in normal tissues (P＜0.05); the level of miR-150 gradually decreased with the aggravation of the disease (P＜0.05), and the level of CXCL8 protein gradually increased with the aggravation of the disease (P＜0.05); Pearson results showed that there was a negative correlation between miR-150 level in patients with psoriasis vulgaris and CXCL8 protein level and PASI score (r=-0.467, -0.475, P＜0.05), and there was a positive correlation between CXCL8 protein level and PASI score (r=0.424, P＜0.05). Conclusions The expression of miR-150 decreased and CXCL8 increased in patients with psoriasis vulgaris, both of them were related to the severity of the disease.

Key words: psoriasis vulgaris, CXC chemokine ligand 8, miR-150, severity of illness