基础医学与临床 ›› 2023, Vol. 43 ›› Issue (2): 289-292.doi: 10.16352/j.issn.1001-6325.2023.02.289

• 临床研究 • 上一篇    下一篇

血浆B细胞趋化因子1和干细胞因子水平与HBV感染患者临床指标的关系

亢必勃1, 赵绒2, 赵杨1, 申焕君1, 陈朝霞1, 周云1, 范超1*   

  1. 空军军医大学第二附属医院 1.感染科; 2.检验科, 陕西 西安 710038
  • 收稿日期:2021-11-19 修回日期:2022-05-06 出版日期:2023-02-05 发布日期:2023-02-02
  • 通讯作者: *fanchao83@163.com
  • 基金资助:
    国家自然科学基金(81670529)

Correlation of serum B cell-attracting chemokine 1 and stem cell factor with clinical indices of HBV infected patients

KANG Bibo1, ZHAO Rong2, ZHAO yang1, SHEN Huanjun1, CHEN Zhaoxia1, ZHOU Yun, FAN Chao1*   

  1. 1. Department of Infectious Diseases;
    2. Department of Clinical Laborator, the Second Affiliated Hospital of Air Force Medical University, Xi'an 710038, China
  • Received:2021-11-19 Revised:2022-05-06 Online:2023-02-05 Published:2023-02-02
  • Contact: *fanchao83@163.com

摘要: 目的 乙型肝炎病毒(HBV)感染者肝脏炎性反应状态与病毒感染指标的相关性。方法 收集HBV感染者及健康者共198例。HBV感染者按谷丙转氨酶/谷草转氨酶(ALT/AST)、乙肝病毒脱氧核糖核酸(HBV-DNA)、乙肝病毒E抗原(HBeAg)及肝硬化等指标进行分组;ELISA检测外周血B细胞趋化因子1(BCA-1)和干细胞因子(SCF)水平;比较BCA-1和SCF在健康者组、不同HBV感染者分组中的差异,并分析其与ALT/AST、HBV-DNA、HBeAg间的相关性;符合正态分布组采用独立样本t检验,非正态分布组采用Mann-Whitney U检验,相关性分析采用Spearman分析。结果 HBV感染者血清的BCA-1和SCF水平均显著高于对照组(P<0.001)。HBV感染合并肝硬化患者SCF水平显著高于HBV感染(无肝硬化)者(P<0.01)。BCA-1水平在HBV-DNA(+/-)及甲胎蛋白(AFP)(正常/异常)组间均存在差异(P<0.05),HBV-DNA阳性患者BCA-1水平高于HBV-DNA阴性患者,AFP异常患者BCA-1水平显著低于AFP正常患者。BCA-1与HBV-DNA、HBeAg均存在正相关( R=0.314; R=0.456; P<0.01)。结论 HBV感染者外周血BCA-1与HBV DNA水平紧密相关,SCF水平在HBV感染肝硬化患者中明显升高,可能具有潜在的临床诊断价值。

关键词: 乙型肝炎病毒感染, B细胞趋化因子1, 干细胞因子, 血清因子检测

Abstract: Objective To explore potential correlation between liver inflammatory response and viral infection index in patients with hepatitis B virus(HBV). Methods Totally 198 serum samples from HBV infected patients and healthy subjects were collected. Patients were divided into different groups according to alanine transaminase/aspartate transaminase(ALT/AST), HBV-DNA, hepatitis B E Antigen(HBeAg) and liver cirrhosis situation; ELISA was used to determine the B cell-attracting chemokine 1(BCA-1) and stem cell factor(SCF) level; the relationship between BCA-1, SCF and other clinical indicators, including ALT/AST, HBV-DNA, HBeAg and cirrhosis condition, were analyzed in different groups; the data was analyzed either with unpaired t test, or with Mann-Whitney U test according to normal distribution, and Spearman method was used for correlation analysis. Results The levels of serum BCA-1 and SCF in HBV infected patients were significantly higher than those in healthy controls(P<0.001). The serum SCF level was significantly higher in cirrhosis patients than patients without cirrhosis(P<0.01). The BCA-1 level was significantly different between HBV-DNA+ and HBV-patients, as well as between alpha-fetoprotein(AFP) normal and abnormal patients(P<0.05). BCA-1 was significantly higher in HBV-DNA+ patients than that in HBV-DNA-patients and was lower in AFP abnormal patients than in normal patients. Furthermore, BCA-1 was positively relate to HBV-DNA and HBeAg level(R=0.314, R=0.456,P<0.01). Conclusions BCA-1 was significantly related to HBV DNA, while SCF is upregulated in cirrhosis patients with HBV infection, which may be the potential candidate of clinical indicators for HBV infection.

Key words: HBV infection, B cell-attracting chemokine 1(BCA-1), stem cell factor(SCF), serum factor detection

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