中国现代神经疾病杂志 ›› 2023, Vol. 23 ›› Issue (6): 479-484. doi: 10.3969/j.issn.1672-6731.2023.06.002

• 专题综述 • 上一篇    下一篇

2 宏基因组第二代测序技术在重症中枢神经系统感染中的应用

杜倩, 邓素君, 邹姗, 金珺, 周青山*()   

  1. 518053 香港大学深圳医院成人重症医学科
  • 收稿日期:2023-04-15 出版日期:2023-06-25 发布日期:2023-07-05
  • 通讯作者: 周青山

Research progress on metagenomic next-generation sequencing in diagnosis of severe central nervous system infection

Qian DU, Su-jun DENG, Shan ZOU, Jun JIN, Qing-shan ZHOU*()   

  1. Department of Adult Intensive Care Unit, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, Guangdong, China
  • Received:2023-04-15 Online:2023-06-25 Published:2023-07-05
  • Contact: Qing-shan ZHOU

摘要:

重症中枢神经系统感染病死率较高,早期识别病原体并采取针对性措施是降低病死率的关键。传统病原学检测技术因自身技术缺陷难以满足临床需要,宏基因组第二代测序技术(mNGS)因快速、高通量、覆盖病原微生物范围广的特点,在重症中枢神经系统感染的应用中得到广泛关注。本文综述mNGS测序发展历程及其在重症中枢神经系统感染中的应用进展,为疾病的精准诊断与治疗提供理论依据。

关键词: 宏基因组, 基因检测, 中枢神经系统感染, 综述

Abstract:

The mortality rate of severe central nervous system infection is high. Early identifying pathogens and giving targeted antibiotics are critical to reduce the mortality rate. Traditional pathogen detection technologies are difficult to meet clinical requirements due to technical limitations. Recently, metagenomic next-generation sequencing (mNGS) has received widespread attention in the infectious field due to its efficiency, high throughput and wide coverage of microorganisms. This article reviews the development of mNGS and its application in severe central nervous system infection, so as to provide a more powerful guarantee for accurate diagnosis and treatment of severe central nervous system infection.

Key words: Metagenome, Genetic testing, Central nervous system infections, Review