摘要： 目的 探讨人软骨糖蛋白39（YKL-40）在帕金森病诊断中的临床价值。方法 酶联免疫吸附试验检测63 例帕金森病患者血清YKL-40 表达水平，统一帕金森病评价量表（UPDRS）和Hoehn-Yahr 分期评价患者日常生活活动能力、运动功能和病情严重程度，Spearman 秩相关分析比较YKL-40 表达水平与帕金森病之关联性，受试者工作特征（ROC）曲线评价YKL-40 诊断帕金森病敏感性和特异性。结果 帕金森病组患者血清YKL-40 表达水平［（3.72 ± 0.10）ng/ml］高于对照组［（2.92 ± 0.18）ng/ml］且差异有统计学意义（t = 4.141，P = 0.000）。血清YKL-40 表达水平与UPDRS 评分（r_s = 0.872，P = 0.000）和Hoehn-Yahr 分期（r_s = 0.704，P = 0.000）均呈正相关。ROC 曲线显示YKL-40 诊断帕金森病之灵敏度为95.26%、特异度73.34%。结论 血清YKL-40 表达变化与帕金森病病情严重程度有关，可以作为诊断帕金森病的实验室参考指标之一。

关键词: 帕金森病, 糖蛋白类

Abstract: Objective  To investigate the value of YKL-40 in diagnosing Parkinson's disease (PD).  Methods  Blood samples of 63 PD patients and 60 healthy controls were collected in this study. YKL-40 levels were measured by enzyme-linked immunosorbent assay (ELISA). Unified Parkinson's Disease Rating Scale (UPDRS) and Hoehn-Yahr (H-Y) stage were used to assess the ability of daily life, motor funciton and disease severity of PD patients. Then the correlation between YKL-40 and motor symptoms of PD was analyzed by Spearman's rank correlation analysis. Receiver operating characteristic (ROC) curve was used to investigate the sensitivity and specificity of YKL-40 in diagnosing PD.  Results  The YKL-40 levels in PD patients were (3.72 ± 0.10) ng/ml, which was significantly higher than that in healthy controls [(2.92 ± 0.18) ng/ml; t = 4.141, P = 0.000]. Spearman analysis indicated that there was positive correlation between YKL-40 and UPDRS scores (r_s = 0.872, P = 0.000), and between YKL-40 and H-Y stage (r_s = 0.704, P = 0.000). ROC curve indicated that the sensitivity and specificity of YKL-40 in diagnosing PD was 95.26% and 73.34% , respectively.  Conclusions  There was a correlation between YKL-40 and the severity of PD, so YKL-40 can be used as a biomarker in diagnosing PD.

Key words: Parkinson disease, Glycoproteins