Table of Content

05 August 2016, Volume 36 Issue 8

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Selenite induced autophagy-dependent caspase 8 activation through accumulating IKK α in the nucleus of Jurkat cells

2016, 36(8):  1035-1039. 

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Objective The objective of our study is to explore the mechanisms by which selenite at super-nutritional range induced autophagy and apoptosis. Methods Western blot was used to detect the alteration of IKK α, P73, UVRAG and apoptotic markers(such as caspase 8) , while transfection assays were carried out to determine the essential role of signal molecules in regulating cell fate. Indirect immunofluorescent assay was used to judge the alteration of protein location. Results 20 μmol/L selenite promoted Jurkat cells undergoing caspase 8-related apoptosis and autophagy(P<0.05), while autophagy was necessary for the cleavage of caspase 8(P<0.05). Further experiments discovered that selenite regulated the contents of P73 and UVRAG(P<0.05), a maker of autophagy, through accumulating IKK α in the nucleus of Jurkat cells(P<0.05). Conclusions Super-nutritional selenite induced autophagy-dependent caspase 8 activation and apoptosis through accumulating IKK α in the nucleus.

PPAR-γ activation inhibits angiotensin II-induced Ets-1 expression in cardiac fibroblasts of rats

2016, 36(8):  1040-1045. 

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Objective To investigate whether peroxisome proliferator-activated receptor-γ (PPAR-γ) activation inhibits cardiac fibrosis through the angiotensin II (Ang II)-Ets-1 pathway. Methods Primary cultured cardiac fibroblasts (CFs) of rats were divided into these groups: control, Ang II, Ang II+ rosiglitazone, Ang II+ other PPAR-γ ligands, Ang II+ PPAR-γ antagonists. The change in expression of Ets-1, connective tissue growth factor (CTGF), transforming growth factor (TGF)-β1 and Smad2/3 were assessed by using real-time RT-PCR and western blotting. Results In growth-arrested CFs, Ang II induced the expression of Ets-1 mRNA and protein（P < 0.05）, up-regulated expression of Ets-1 down stream target CTGF (P < 0.05), enhanced the expression of TGF-β1 and the expression and phosphorylation of Smad2/3 (P < 0.05). PPAR-γ ligands rosiglitazone and 15d-PGJ2 attenuated the expression of Ang II-induced Ets-1 mRNA and protein (P < 0.05), decreased the induction of CTGF by Ang II (P < 0.05), inhibited in part the expression of TGF-β1 and the expression and phosphorylation of Smad2/3 induced by Ang II (P < 0.05). These suppressive effects on Ets-1 and CTGF were attenuated by PPAR-γ antagonists GW9662 and BADGE (P < 0.05). Conclusions Activation of PPAR-γ inhibits Ang II-induced Ets-1 expression via the TGF-β1/Smad2/3 signaling pathway.

Correlation between total IgE and IgE anti-BP180 or anti-BP230 autoantibodies in patients with bullous pemphigoidi

2016, 36(8):  1046-1050. 

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Objectives To examine the levels of blood eosinophil counts, total IgE and IgE autoantibodies against BP180 and BP230 in sera of bullous pemphigoid（BP）, and to analyze correlations. Methods Sera obtained from 63 BP patients were subjected to measure serum levels of total IgE, anti-BP180 IgE and anti-BP230 IgE antibodies by ELISA assays. And analyse the relevance between Blood eosinophils/ bullous pemphigoid disease area index(BPDAI) scores and IgE autoantibodies. Results Total IgE, IgE anti-BP180 and IgE anti-BP230 antibodies were positive in 41 (65.08%),37 (58.73%) and 47 (74.60%) case of BP patient respectively. Anti-BP230 IgE levels showed a strong association with total IgE（r=0.643，p<0.001）. Of 63 patients with BP, 34 were recorded blood eosinophil counts. Blood eosinophils increased in 19 patients (55.88%). Anti-BP180 IgE were significantly correlated with urticarial/erythema scores（r=0.934，p＜0.05）. Conclusions IgE plays a role in the pathogenesis of BP. IgE autoantibodies may primarily target to BP230. Anti-BP180 IgE may effect on the urticarial lesions.

Apelin Inhibits TGFβ-induced human renal tubular epithelial-mesenchymal transition

2016, 36(8):  1051-1056. 

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Objectives In this study, inhibitory effects of apelin on transforming growth factor-β (TGF-β)-induced epithelial-mesenchymal transition (EMT) in human proximal renal tubular epithelial cells were examined and related mechanism was elucidated. Methods Human proximal renal tubular epithelial cells were cultured in vitro. Cells were incubated with TGF-β1 (2 μg/L) and/or different concentrations of apelin-13 for 48 hours. Six groups were established (n=5 in each group): Control group, TGF-β group, TGF-β+Apelin (10-8, 10-7 and 10-6mol/L) group, apelin (10-6mol/L) group. Immunofluorescence was performed to visualize the distribution of epithelial marker E-cadherin and mesenchymal marker α-smooth muscle actin (α-SMA). Western blot analysis was performed to determine the level of E-cadherin, α-SMA, and expression of key Smads signaling molecules p-Smad2/3, Smad2/3 and Smad-7. RT-PCR analysis was performed to evaluate the mRNA level of fibronectin and CollagenⅠ, and expression of endogenous apelin and APJ. Results Compared with control group, cells in TGF-β group was long spindle shape, together with decreased expression of E-cadherin and increased expression of α-SMA. Morever, transcripts of fibronectin and collagen I mRNAs were also increased in TGF-β group. However, these effects were obviously inhibited in TGFβ+Apelin group in a concentration-dependent manner. Compared with TGF-β group, the level of p-Smad2/3 was decreased (P<0.05), while the level of Smad7 was increased (P<0.05) in TGF-β+Apelin group . In TGF-β group, expression of APJ was upregulated (P<0.05). Remarkably, this effect was also inhibited in TGFβ+Apelin group in a concentration-dependent manner. Conclusions This study provides the first evidence that apelin is able to protect against tubular EMT through antagonism of TGF-β/Smads pathway. The endogenous apelinergic system may promote some compensatory response in tubular EMT process.

Shikonin inhibits the proliferation of NB4 cells via down-regulation of c-MYC

2016, 36(8):  1057-1061. 

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Objective To investigate the effect of shikoninon proliferation of leukemic NB4 cells andthe related mechanism. Methods CCK-8 assay was used to examine the proliferation inhibition of shikonin on NB4 cells. Cell cycle and apoptosis was detected by flow cytometry. Theexpression of c-MYC, ERK and p-ERK were detected by Western blot. The data was analyzed by one -wayANOVA test and ttest using SPSS17.0 softwarepackage.Results Shikonininhibited NB4 cell proliferation in concentration and time dependentmanner(P<0.01). The percentage of cells in G1 phase was increased while was decreased in G2 and S phases(P<0.01). The rate of apoptosis in the shikonin group was obviously higher than control group(P<0.01). Theexpression of c-MYC wasdecreased(P<0.05) and p-ERK was increased(P<0.01). ConclusionsShikoin can inhibit NB4 cells proliferationthrough down-regulation of c-MYC with involvement of ERK pathways.

Effect of iron on the vasculogenic mimicry in triple negative breast cancer cells in vitro

2016, 36(8):  1062-1067. 

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Objective: To study the effect and possible mechanism of iron induced vasculogenic mimicry (VM) formation in breast cancer cell. Methods: We devided MDA-MB-231 cells into seven groups: control group, 500μmol/L DFO group, 300μmol/L and 30μmol/L mimosine group, 50μmol/L and 500μmol/L ferrous lactate group. MTT was used to observe the proliferation of breast cancer cell. Formation of VM was identified by both 3D culture and PAS stain method. Change of ROS in cells was measured by immunofluoresence staining method. Under difference concentrations of iron, the expression of p-ERK1/2 in MDA-MB-231 was tested by Western blot. Results: Pipeline structure was obviously inhibited in vitro in DFO- and mimosine-treated groups(P<0.05), while ferrous lactate promoted the formation of vessel-like structure(P<0.05). PAS staining demonstated that vessel-like structure was VM. Immunofluoresence staining and Western blot showed that ROS in cells were consistent with the expression of p-ERK1/2. Specific inhibitor (PD98059) significantly decreased the formation of VM(P<0.05). Conclusions: ROS is positively involved in iron-induced VM formation. Abnormal activation of ERK1/2 pathway may be the principal mechanism of regulation of ROS.

Development of OGDH gene knockout SD rat model

2016, 36(8):  1068-1073. 

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Objective: To establish OGDH gene knockout SD rats by transcription activator-like effector nucleases (TALEN) technology. Methods: Three pairs of TALEN plasmid were designed and constructed, then the transfection activity was examined in 293T cells, and the TALEN pair with the most effective mutation result was selected before the plasmid pair was applied to the fertilized rat eggs using microinjection. DNA sequencing with tissue samples from two-weeks newborn SD rats were performed. The SD rats were followed by measuring the body weight, observing food taking, running activity and fur color etc. Theprotein expression of OGDH was detected by Western Blot. Results: The TALEN plasmid pair OGDH-T2 was found the most effective and this plasmid pair was used in this study.In total, 80 fertilized eggs were injected with the OGDH-T2 plasmid pair and 18 rats were born, with an efficiency rate of 22.5%. However, homogeneous knockout rats were notobtained after followed a period of three generations. However, heterogeneous KO mutation OGDH-KO8 could be consistently maintained. Therefore, we followed this type of heterogeneous KO rats in this study. We discovered that OGDH-KO8 influenced the bodyweight. Compared with the control rats, the rats with the OGDH-KO8 heterogeneous mutation showed significantly higher body weight within the 10 weeks′observation(P<0.001), Otherwise, there was no difference observed in this typical heterogeneous KO rats. In consistence with the bodyweight increase, the OGDH protein expression was decreased in theserats, the rates of decreased up to 50.6%(P<0.01), the expression of liver OGDH protein is lower 50.6% than the WT type.Conclusion: Heterogeneous OGDH gene knockout SD rats are successfully established.

Interaction between ribonuclease inhibitor and integrin-linked kinase and its effect on angiogenesis in vitro

2016, 36(8):  1074-1079. 

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Objective To research the interaction between ribonuclease inhibitor(RI) and integrin-linked kinase(ILK) and its effect on angiogenesis in vitro. Methods To construct YFP-ILK and pCMV-3×flag-ILK plasmid. Purchase lentiviral expres-sion vector LV5-RNH1 homo and LV5NC.Stably transfect vectors into EJ cells repectively, and the selected cells were named as EJ-RI, EJ-ILK, EJ-LV5, EJ-FLAG and EJ. Fluorescence microscope analysis was used to observe the co-location between RI and ILK in 293 cells and EJ cells. The interaction between RI and ILK in 293 and EJ cells was identified by glutathione-S-transferase (GST) pull-down assay and co-immunoprecipitation assay. Endothelial tube formation assay was used to assess angiogenesis in vitro. Results The expressing plamid YFP-ILK and pCMV-3×flag-ILK were verified by restriction enzyme digestion and DNA sequencing.We found that RI was co-localized with ILK in 293 and EJ cell. Glutathione-S-transferase (GST) pull-down and co-immunoprecipitation results showed that the interaction between RI and ILK occurred in prokaryotic cells and mammalian cells as well. RI can inhibit angiogenesis in vitro by interacting with ILK.Conclusion The interaction between RI and ILK occurs in prokatyotic cells and mammalian cells and inhibits angiogenesis in vitro.

Clostridium butyricumculture supernatant inhibits proliferation of SW-480 cells through inhibition of TLR4/NF-κB pathway

2016, 36(8):  1080-1086. 

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ObjectiveTo study the effects of proliferation inhibitionof C.butyricum culture supernatant (C.b.cs) on colon cancer SW-480 cells,and to explore the possible active ingredient and related molecular mechanism. MethodsCCK-8 and FCM wereused to test the cell proliferation and apoptosis.Furthermore, high efficiency liquid chromatography (HPLC) was used to analyze the concentrations of the main organic acid in C.b.cs. After the stimulation of lipopolysaccharide (LPS), the expression of TLR4 was detected by Western blot and qRT-PCR after the treatment of C.b.cs andbutyrate.ResultsC.b.cs inhibits the proliferation of SW-480cells in dose-dependent manner, which can also induce apoptosis and G0/G1 phase arrest. HPLC analysis showed that the concentrations of acetic acid and butyric acid were 9.27g/L and 4.53g/Lin C.b.cs respectively. The expression of TLR4 was inhibited after the treatment of C.b.cs and butyrate（p<0.01）,as the downstream gene of TLR4, the expression of NF-κBwas also inhibited（p<0.01）.Furtherly, the expression of TLR4 was regulated by LPS（p<0.01）, and C.b.cs and butyrateattenuatedthe upregulation（p<0.05）. ConclusionsC.b.cs and butyrate inhibit SW-480 cell proliferation by downregulation the expression of TLR4/NF-κB pathway.

Inhibition of autophagy increases the sensitivity of breast cancer MCF-7 cells to etoposide

2016, 36(8):  1087-1091. 

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Objective To investigate the effects of bafilomycin A1(Baf) on the sensitivity of MCF-7 breast cancer cells to etoposide (VP-16). Methods The cultured cells were divided into normal control (NC), Baf , Vp-16 and VP-16 + Baf groups. The viability of cells was determined with MTT assay. After GFP-LC3 plasmid transfection to the cells, the distribution of green fluorescence was observed by using fluorescence microscopy. The protein expression was assayed by Western blot and the apoptosis of MCF-7 cells was detected by flow cytometry. Results VP-16 at 15μmol/L reduced the viability of cells. Baf (10nmol/L) which was added to treat the cells for 12h before the cells collection reduced the viability of cells more than VP-16 used only(P<0.01). VP-16 obviously induced the expression of LC3Ⅱ and GFP-LC3 dots in MCF-7 cells, and decreased the expression of p62. Baf inhibited the autophagy induced by VP-16, and increased the expression of cleaved-PARP in MCF-7 cells and the apoptotic rates (P<0.01). Conclusion VP-16 reduced the viability of breast cancer cells and induced protective autophagy, and inhibition of autophagy promoted the apoptotic death and increased the sensitivity of cells to VP-16.

Effect of Gli2 gene silencing on the proliferation,migration and invasion in colon cancer cell line SW480

2016, 36(8):  1092-1097. 

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bjective To explore the effect and mechanism of Gli2 on the proliferation,migration and invasion in colon cancer cell line SW480.Methods Complementary shRNA oligonucleotides targeting the Gli2 gene was designed and inserted into lentiviral vector. Then shRNA lentivirus were transfected into SW480 cells and then fluorescence photographs were taken. The gene silencing efficiency was measured by qRT-PCR and Western blot. Interfered group,negative group and blank group were set up.MTT,doubling time and colony-forming test were used to examine the proliferation of SW480.Migration and invasion ability were evaluated by Transwell chambers assay;Meanwhile,the qRT-PCR、Western blot were used to examine cyclinD1 and MMP-2 mRNA and protein expression. Results Green fluorescent protein was observed under fluorescence microscope after 72h in SW480 cells transfected with lentivirus.Compared with the negative control group and blank control group,the expression of Gli2 in the interfered group was lower,the cells grew slower,the doubling time was longer and the colony-forming ability was decreased,migration and invasion ability were weaken,meanwhile,the expression of cyclinD1 and MMP-2 was declined significantly(p<0.05). Conclusion Silencing Gli2 gene could suppress proliferation,migration and invasion ability in colon cancer cell line SW480.its mechanism may be related to the down-regulation of cyclinD1 and MMP-2.

Investigation and analysis of 16 population genetic characters of Bai people in Guizhou

2016, 36(8):  1098-1101. 

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Objective About 16 population genetic characteristics of Bai in Guizhou. Methods A survey of 406 parents were 16 population genetic traits of the same nationality of Bai in Guizhou.ResultsGuizhou Bai hair color was black, tuorbillion as cum sole,Many people as long eyelashes, Eye color as black, direction of eye crack as Level and oblique close to,Wide Nostril type more than narrow,A lot ofstraight nose,ceruminous as dry typemore than wet type, thick lips as thin, mouth breadth as narrow, Front tooth type as shovel type,retroflexion and nonclose to,much as tongue folding,a lot of pass thyough,have middle finger hair than more non,a lot of as two toe ength.The16 genetic characteristics had no significant difference between male and female of Baiin Guizhou (p>0.05),Thereare 24Compound feature was relevant. Conclusions The characteristics of population genetics has specific national and regional differences,And has a certain correlation among the features.

Bidirectional effect of 5-HT on the spontaneous discharge of subthalamic nucleus neurons in normal rats

2016, 36(8):  1102-1107. 

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Objective The aim was to study the effects and mechanism of 5-HT on the firing rate of subthalamic neurons in normal rats. Methods With in vivo extracellular single unit recordings, the electrophysiological effects of 5-HT and 5-HT1B, 5-HT2C, 5-HT4 and 5-HT1A receptor agonist on subthalamic neurons were studied. Then to observe the expression of four kinds of 5-HT receptor subtypes on subthalamic nucleus neurons, the immunohistochemical staining was used in the present study. Results Electrophysiological studies showed that exogenous 5-HT increased or decreased the discharge frequency of subthalamic nucleus neurons in normal rats (P<0.001). As 5-HT receptor agonists, CP-93129, RO-600175 and ML-10302 significantly increased the firing rate of subthalamic neurons (CP-93129: P<0.01; RO-600175: P<0.01; ML-10302: P<0.001), while 8-OH-DPAT decreased the firing rate (P<0.01). Conclusion 5-HT altered the excitability of subthalamic nucleus neurons in normal rats, resulting in the dual role of raising and lowering the discharge frequency. The activation of 5-HT1B, 5-HT2C and 5-HT4 receptor increased the firing rate of subthalamic nucleus neurons, while when 5-HT1A receptor was activated, the firing rate was decreased.

Effects of Resveratrol on Bcl-2/Bax and mitochondrial membrane potential of cells A?1-42 induced HUVECs

2016, 36(8):  1108-1112. 

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Objective: To investigate the effects of resveratrol on Bcl-2/Bax expression, mitochondrial membrane potential and the NF-κB nuclear translocation of the HUVEC cells induced by A?1-42.Methods: HUVEC cells were stimulatedby A?1-42 5×101μmol/L 24 hours, 160,80,40,20μg/L resveratrol were adminstrated,cell viability was observed by CCK-8; NF-κB nuclear translocation and mitochondrial membrane potential were tested by the fluorescence microscope,expression of NF-κB/Bcl-2/Bax protein were detected by Western blot.Results: Compared with A?1-42 group, resveratrol could increase the cell viability and the mitochondrial membrane potential,inhibited the NF-κB nuclear translocation , and reduced the expression of NF-κB in nucleus,reduced the expression of Bax,increased the expression of Bcl-2 ,increased the Bcl-2/Bax ratio significantly (P<0.05). Conclusion: Resveratrol could against the injure of HUVEC cell induced by A?1-42, and the mechanism may be associated with inhibitig the NF-κB nuclear translocation ,increasing the mitochondrial membrane potential and increased the Bcl-2/Bax ratio.

Tanshinone IIA attenuates pulmonary fibrosis through iNOS in mice

2016, 36(8):  1113-1117. 

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Objective To investigate the mechanism ofTanshinone IIA in treating with pulmonary fibrosis in mice. Methods A549 cells and Bleomycin induced pulmonary fibrosis mice were divided into control group, pulmonary fibrosis model group and Tanshinone IIA group, observe iNOS expression between different groups by PCR and western blot. Detect the down-stream cytokines TNF-α,IL-1βand IL-6 by using ELISA. Results Histopathology showed that pulmonary fibrosis group mice had more severe fiber foci in alveolar septum, lesions, structural damage to the lung parenchyma.The in vitro experiment showed a inhibition effect of Tanshinone IIA on iNOS expression(P<0.05).TNF-α,IL-1βand IL-6 expressions decreased significantly compared to bleomycin group (P<0.05).Conclusion Tanshinone IIA can reduce pulmonary fibrosisin mice by regulating iNOS expression.

Effects of silencing BMP4 on mesenchymal epithelial transition, invasion and migration ability of osteosarcoma cell line MG-63

2016, 36(8):  1118-1123. 

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Objective To investigate the effects of silencing BMP4 on mesenchymal epithelial transition, invasion and migration ability of osteosarcoma cell line MG-63. Methods BMP4 shRNA plasmid was transfected into MG-63 cells,MG-63 cells were divided into MG-63 control group(normal culture control)，empty control group（transfected empty plasmid），MG-63silence group（transfected BMP4 silence shRNA）. Reverse transcription polymerase chain reaction （RT-PCR） and Western blotting were used to detect the changes of E-cadherin，vimentin、twist and snail mRNA and protein levels after the cells BMP4 was silenced. Transwell invasion assay and wound healing assay were conducted when the BPM4 was silenced to detect the changes of the invasion and migration ability of MG-63 cells. Results Silencing BMP4 decreased MG-63 cells twist and snail expression, increased the expression of epithelial cell marker E-cadherin, and decreased expression of vimentin. Invasion and migration assays showed that the transmembrane cell number and migration distance of BMP4 silence group were significantly less than those of the MG-63 control group and empty control group（P<0.05）.Conclusions The silence of BMP4 gene may change MG-63 osteosarcoma cells from mesenchymal phenotype to epithelial phenotype, reducing tumor cell invasion and migration ability effectively.

Construction of miR-181b lentiviral vector and its targeting effect on MAPK1 gene

2016, 36(8):  1124-1129. 

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Objective To construct alentiviral vector of miR-181b andverify its targeted effect on MAPK1 gene. MethodspSicoR-miR-181b and pMIR-Report-MAPK1 over-expression vector were construsted.ThemRNA of MAPK1 was verified by real-time PCR testand the protein level of MAPK1 was verified by Western blot .To validate the targeted regulatory relationship between miR-181band MAPK1 3’-UTRthrough relative luciferase activity.ResultsTherecombinant plasmids of pSicoR-miR-181b and pMIR-Report-MAPK13'-UTR were constructedsuccessfully. And it was showed that over-expression of miR-181b suppressed the mRNA and protein expression level of MAPK1significantly(p<0.05),suppression of miR-181b expression increased the expression level of MAPK1 mRNA and protein significantly(p<0.05). ConclusionsThelentiviral vector containing miR-181b gene has successfully constructed.MiR-181b can suppress MAPK1 gene expression by targeting the specific sequence ofMAPK1-3'UTR.

Establishment of a low-serum inducing method for the differentiation of human embryonic stem cells into mesenchymal stem cells

Nian-Hua FENG Xue-Bin QU

2016, 36(8):  1130-1134. 

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Objective To establish a differentiation inducing method of mesenchymal stem cells (MSCs) from human embryonic stem cells (hESCs) using low-serum containing medium. Method The induction was initiated by culturing human embryonic stem cells in low-serum containing medium, the medium was half-changed every 3 days. 7～10 days later, cells were confluent and dissociated into single cells, then culture medium was changed to proliferation medium, cells were subcultured every 3 days. Results hESCs were cultured in feeder free condition and grew as clones with serrated edges, all clones expressed pluripotency markes Oct4, Sox2 and Nanog. After 3 days’induction, fibroblast-like cells with small volume appeared between hESCs clones. These cells grew as clusters and possessed high proliferation potential. Cells with uniform spindly morphology were obtained after 2 passage. All cells expressed mesenchymal marker-vimentin. Flowcytometry analysis showed that these cells expressed CD105, CD90, CD73, CD44 and HLA-ABC, no expression of CD34, CD45 and HLA-DR was detected. Under certain condition, these cells could differentiate into osteoblasts and fat cells. Conclusion Human embryonic stem cell derived mesenchymal stem cells (hESC-MSCs) with high purity can be obtained in a short time by using low-serum containing induction medium.

Evaluation of image quality of abdominal aorta and lower extremity artery CTA under 80kVp tube voltage with ATCM

2016, 36(8):  1135-1138. 

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Objective The aim of the research was to compare the radiation dose and image quality of abdominal aorta and lower extremity artery CTA under 80kV and 120kV，to evaluate the feasibility of low tube voltage abdominal aorta and lower extremity artery CTA. Methods It was a retrospective research, there were 34 patients who were divided into two groups with 80kV or 120kV tuber voltage separately received abdominal aorta and lower extremity CTA. The objective and subjective evaluations were done based on axial and 3D reconstruction images. The data was analyzed by Shapiro-WilkNormal distribution test and independent t test. Results The CT number of arteries under 80kV tube voltage was between 542HU to 601HU, which were much higher than that under 120kV tube voltage. There were no statistical difference between CNR and objective evaluation between the two groups. The DLP of group A with 80kV tube voltage was 205.94±20.69 mGy·cm, and which was 775.47±82.85 mGy·cm of group B with 120kV tube voltage. The DLP decreased 73%. Conclusion The image quality of abdominal aorta and lower extremity artery CTA under 80kV tube voltage with ATCM could meet the need of clinical diagnose.

Proliferation inhibition and autophagy induction of PI3K/mTOR inhibitor NVP-BEZ235 in human breast cancer cells

2016, 36(8):  1139-1141. 

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CXCL-8 promotes human fibroblasts migration in high glucose environment

2016, 36(8):  1142-1143. 

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Analysis a allele HLA- A*02:446 and investigate of it′s family heritage

2016, 36(8):  1144-1146. 

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Astragaloside A relieve gastric mucous membrance change in rats

2016, 36(8):  1147-1149. 

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Advance of long non-coding RNAH19 in cancer

2016, 36(8):  1150-1153. 

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Long non-coding RNA(LncRNA) H19 associates with cancer. It plays a cancer promoting role in lung cancer, breast cancer and stomach cancer, but H19 has dual effect in hepatocellular carcinoma. The mechanism of H19 is still unclear. It may regulated by miR-675, expression of oncogenes, formatting of EMT and the imprinting status of IGF2 under methylation. H19 might be a valuable potential biomarker of cancer.

The role of melatonin receptor signal in type 2 diabetes

2016, 36(8):  1154-1158. 

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Melatonin is a hormone secreted by the pineal gland, and regulates many physiological conditions including sleep. Melatonin specifically binds to melatonin receptors, regulating central and peripheral functions. The melatonin receptor-mediated signaling pathways in pancreatic β cell affect pancreatic insulin secretion and the function of pancreatic β cell. The activation of melatonin receptors increases the arylalkylamine-N-acetyltransferase activity, which promotes secretion of melatonin, further inhibiting insulin secretion and elevating blood sugar.

Long non-coding RNA and colorectal cancer

2016, 36(8):  1159-1163. 

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Long non-coding RNA (lncRNA, >200 nt)can interact with DNA, RNA, protein molecules and/or their combinations. lncRNA is an essential regulator in transcriptional and post-transcriptional regulation and act as tumor promoter or suppresser in colorectal cancer. Misexpression or mutation of lncRNA play an important role in cancer genesis, development, invasion and metastasis and close related to patient prognosis. Taken together, lncRNA may be an important target for colorectal cancer diagnosis and treatment.

Recent advance in surgical management of idiopathic normal pressure hydrocephalus

Ren-zhi WANG, Jun-ji WEI,

2016, 36(8):  1164-1167. 

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Idiopathic normal pressure hydrocephalus (iNPH) is a syndrome characterized by the clinical triad of gait disturbance, dementia, urinary incontinence, with ventricular dilation and normal cerebrospinal fluid pressure (CSF), and these symptoms could be revered by CSF shunt surgery. However clinical symptoms will gradually deteriorate without surgical management. Ventriculoperitoneal shunt（VPS） is the most common surgical procedure. Prompt diagnosis and treatment can significantly improve the patient's condition. The prognosis is satisfied,if the patients diagnosed as iNPH are treated as early as possible.

The practice of student-oriented examination reform of pathophysiology

2016, 36(8):  1168-1171. 

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Objective Pathophysiology is a bridge subject between basic medicine and clinical discipline. As an important part of teaching, the examination system guarantees the education quality. Thus, examination reform is imperative. Methods Following the PUHSC’s student-oriented idea, department of Physiology and Pathophysiolgy is committed to promote students’ development and reform the examination system in the 5-year medical program. Classroom theory assessment was implemented with theoretical teaching; experimental course assessment was carried out to assess experiment teaching; situational logic questions were integrated into final theory examination; examination analysis was conducted to strengthen the feedback function of examination. Results: A comprehensive evaluation system combined by classroom theory assessment, experimental course assessment and final theory examination was built up gradually. Conclusion: The examination reform improves the teaching quality of pathophysiology, as well as promote innovative talents.

Application of formative assessment system in medical immunology teaching

2016, 36(8):  1172-1175. 

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In this study, we used formative assessment system in medical immunology teaching evaluation system. We established a teaching evaluation system in a combination of formative assessment and final scoring assessment. We aimed to investigate the application of formative assessment system in the medical immunology teaching. We analyzed the difficulties encountered in the teaching process, and tried to find a solution.

Evaluation of CBL and PBL teaching methods in novitiate stage in hematology for medical undergraduate

2016, 36(8):  1176-1178. 

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Objective To study the application effect of CBL and PBL teaching methods in novitiate stage in Hematology for Medical Undergraduate. Method We used self-control method in this study. Students were randomly divided into two groups, has applied CBL and PBL teaching and traditional teaching. We compared the difference between the results of the second test at the end of probation and combine the views of CBL and PBL. Results compared with the traditional model, CBL and PBL mode in many ways to improve the effectiveness of teaching. Conclusions Most students think trainee method CBL and PBL bound to help improve their level of medical theory, clinical analytical skills and teamwork.