Abstract: Objective To construct alentiviral vector of miR-181b andverify its targeted effect on MAPK1 gene. MethodspSicoR-miR-181b and pMIR-Report-MAPK1 over-expression vector were construsted.ThemRNA of MAPK1 was verified by real-time PCR testand the protein level of MAPK1 was verified by Western blot .To validate the targeted regulatory relationship between miR-181band MAPK1 3’-UTRthrough relative luciferase activity.ResultsTherecombinant plasmids of pSicoR-miR-181b and pMIR-Report-MAPK13'-UTR were constructedsuccessfully. And it was showed that over-expression of miR-181b suppressed the mRNA and protein expression level of MAPK1significantly(p<0.05),suppression of miR-181b expression increased the expression level of MAPK1 mRNA and protein significantly(p<0.05). ConclusionsThelentiviral vector containing miR-181b gene has successfully constructed.MiR-181b can suppress MAPK1 gene expression by targeting the specific sequence ofMAPK1-3'UTR.

Key words: microRNA, miR-181b, MAPK1