Abstract: Objective To investigate distribution of HPV16E6 mutation and whether there are differences in Han, Uygur cervical cancer(CC) and precancerous lesions.the relationship between the mutation and high incidence of CC in Han, Uygur women. Methods 140case precancerous lesions and cervical carcinoma patients HPV16 genotypes was chosen and HPV16E6 gene was amplified by polymerase chain reaction (PCR). The PCR fragments were sequenced and analyzed. Results There was mutations in 123 cases , mutation rate distribution in Han and Uygur were 47.37%（27/57）and 50%( 33/66) respectively . They were detected mutations D25E、D64E、I73V、H78Y、D113E, L83V, the hot mutation was L83V , rates was 29.82%（17/57）in Han significant lower than 40.90%(27/66) in Uygur, Rates was D25E 19.30%（11/57）in Han significant higher than7.58%(5/66) in Uygur . Rates was D64E 6.1% in Uygur , but have no prevented in Han . Conclusion There was difference between HPV16E6 gene mutation sites and rates in two nations .Our research suggested that the distribution of D64E in Uygur might be associated with high incidence of CC in this nation.

Key words: Han , Uighur, cervical cancer and lesions , Human papillomavirus , E6 gene