血浆IgG N-糖肽绝对定量方法用于筛选早期慢性阻塞性肺疾病诊断标志物

doi:10.16352/j.issn.1001-6325.2026.03.0332

基础医学与临床 ›› 2026, Vol. 46 ›› Issue (3): 332-338.doi: 10.16352/j.issn.1001-6325.2026.03.0332

血浆IgG N-糖肽绝对定量方法用于筛选早期慢性阻塞性肺疾病诊断标志物

刘肇一, 尹美玲, 王琳, 周巾煜^*

中国医学科学院北京协和医学院基础医学研究所药理学系重大疾病共性机制研究全国重点实验室,北京 100005

收稿日期:2025-11-06 修回日期:2025-12-24 出版日期:2026-03-05 发布日期:2026-02-25
通讯作者: ^*zhoujinyu@ibms.pumc.edu.cn
基金资助:
国家自然科学基金(22574174)

Absolute quantification of plasma IgG N-glycopeptides as a biomarker for diagnosis of the early COPD

LIU Zhaoyi, YIN Meiling, WANG Lin, ZHOU Jinyu^*

Department of Pharmacology, State Key Laboratory of Common Mechanism Research for Major Diseases, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100005, China

Received:2025-11-06 Revised:2025-12-24 Online:2026-03-05 Published:2026-02-25
Contact: ^*zhoujinyu@ibms.pumc.edu.cn

摘要/Abstract

摘要： 目的探究血浆IgG N-糖肽的绝对定量方法在慢性阻塞性肺疾病 (COPD)早期诊断中的应用价值。方法从既往研究队列中随机选取健康对照(HC)组、早期COPD(ECOPD)组、稳定期COPD(COPD)组研究对象各20例,基于前期获得的血浆IgG N-糖肽相对定量数据,筛选出目标差异糖肽。用内标法实现差异糖肽的绝对定量,探究绝对定量值与1秒钟用力呼气容积与用力肺活量比(FEV1/FVC)的线性关系。采用受试者工作特征(ROC)曲线比较糖肽指标、年龄及吸烟指数的单独及联合诊断效能。结果相对定量结果显示,血浆IgG2 G2FS糖肽水平在HC组显著高于ECOPD组和COPD组(P＜0.05)。绝对定量结果进一步明确,其浓度在3组间呈依次递减趋势,且HC组与COPD组间差异尤为显著(P＜0.001)。ROC曲线分析表明,IgG2 G2FS血浆浓度对ECOPD的诊断效能(曲线下面积AUC=0.765)显著高于年龄(AUC=0.624)和吸烟指数(AUC=0.602)。然而,联合上述指标的诊断价值(AUC=0.780)与单独使用IgG2 G2FS诊断时相当。结论血浆IgG2 G2FS的绝对定量结果具有较高的早期COPD诊断价值,血浆IgG2 G2FS可作为潜在生物标志物。

关键词: 慢性阻塞性肺病, 早期诊断, N-糖基化修饰, 免疫球蛋白, 绝对定量

Abstract: Objective To investigate the potential of the absolute quantifaction of plasma IgG N-glycopeptides for the early diagnosis of chronic obstructive pulmonary disease (COPD). Methods Research objects were randomly selected from the healthy control (HC) group, the early COPD (ECOPD) group and the stable COPD(COPD) group from previous research cohort(20 cases for each group), and targeted differential glycopeptides were selected based on the previously obtained relative quantitative data of plasma IgG N-glycopeptides. Absolute concentration of glycopeptide was measured with internal standard method. The linear relationship between the absolute concentra- tion of the targeted glycopeptides and the ratio of forced expiratory volume in one second to forced vital capacity (FEV1/FVC) was analyzed. The receiver operating characteristic (ROC) curves were generated to compare the diagnostic efficacy of individual glycopeptides indicators, age, smoking index and their combination. Results The relative quantification showed that the plasma level of IgG2 G2FS glycopeptides was significantly higher in the HC group than that in the ECOPD and COPD groups (P＜0.05). The absolute quantification further clarified a successive decrease in its concentration across the HC, ECOPD, and COPD groups, with a particular difference between the HC and COPD groups (P＜0.001). ROC analysis indicated that the diagnostic efficacy of the plasma IgG2 G2FS concentration for ECOPD (area under curve,AUC=0.765) was superior to that of age (AUC=0.624) or smoking index (AUC=0.602). However, the combined model incorporating IgG2 G2FS, age, and smoking index (AUC=0.780) showed comparable performance to IgG2 G2FS alone. Conclusions The absolute concentration of plasma IgG2 G2FS exhibits competitive diagnostic value for early COPD, suggesting plasma IgG2 G2FS is a potential biomarker for early digestion of COPD.

Key words: chronic obstructive pulmonary disease, early diagnosis, N-glycosylation, immunoglobulin, absolute quantification

中图分类号:

刘肇一, 尹美玲, 王琳, 周巾煜. 血浆IgG N-糖肽绝对定量方法用于筛选早期慢性阻塞性肺疾病诊断标志物[J]. 基础医学与临床, 2026, 46(3): 332-338.

LIU Zhaoyi, YIN Meiling, WANG Lin, ZHOU Jinyu. Absolute quantification of plasma IgG N-glycopeptides as a biomarker for diagnosis of the early COPD[J]. Basic & Clinical Medicine, 2026, 46(3): 332-338.

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血浆IgG N-糖肽绝对定量方法用于筛选早期慢性阻塞性肺疾病诊断标志物

Absolute quantification of plasma IgG N-glycopeptides as a biomarker for diagnosis of the early COPD

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