压电响应性水凝胶促进大鼠髓核细胞基质合成

doi:10.16352/j.issn.1001-6325.2026.03.0325

基础医学与临床 ›› 2026, Vol. 46 ›› Issue (3): 325-331.doi: 10.16352/j.issn.1001-6325.2026.03.0325

压电响应性水凝胶促进大鼠髓核细胞基质合成

关云博^1,2,3, 赵子腾³, 张自强^2,3, 孙笑非², 赵彦涛³, 王祖强^1,2,3*

1.安徽医科大学第五临床医学院,安徽合肥 230032;
2.中国人民解放军总医院第六医学中心骨科,北京 100048;
3.中国人民解放军总医院第四医学中心骨科医学部,北京 100048

收稿日期:2025-10-30 修回日期:2025-12-24 出版日期:2026-03-05 发布日期:2026-02-25
通讯作者: ^*wangzqtmmu@hotmail.com
基金资助:
国家自然科学基金(82102594)

Piezoelectric-responsive hydrogel promotes matrix synthesis in nucleus pulposus cells of rats

GUAN Yunbo^1,2,3, ZHAO Ziteng³, ZHANG Ziqiang^2,3, SUN Xiaofei², ZHAO Yantao³, WANG Zuqiang^1,2,3*

1. The Fifth Clinical Medical College of Anhui Medical University, Hefei 230032;
2. Department of Orthopedics, the Sixth Medical Center, General Hospital of Chinese PLA, Beijing 100048;
3. Senior Department of Orthopedics, the Fourth Medical Center, General Hospital of Chinese PLA, Beijing 100048, China

Received:2025-10-30 Revised:2025-12-24 Online:2026-03-05 Published:2026-02-25
Contact: ^*wangzqtmmu@hotmail.com

摘要/Abstract

摘要： 目的探究一种压电响应性水凝胶(KNN/GelMA)的力学、压电、生物相容性等材料基础性能,并研究其压电效应对髓核细胞(NPCs)基质合成代谢的影响。方法 1)材料表征:将铌酸钾钠(KNN)使用超声均匀分散在甲基丙烯酸酸酐明胶(GelMA)水凝胶中,制备光固化可降解压电响应性水凝胶,通过测定微观样貌和压电性能等对材料进行初步的理化表征检测。2)细胞实验:将压电响应性水凝胶与第3代大鼠原代髓核细胞共培养,利用CCK -8法和Calcein/PI细胞活性与细胞毒性检测试剂盒对细胞增殖能力和材料生物相容性进行评价;用RT-qPCR检测软骨细胞外基质合成中相关基因的表达。结果材料表面呈现均匀的孔隙结构,铌酸钾钠(KNN)纳米颗粒均匀分布于水凝胶内部;压电输出实验显示,KNN/GelMA在受力条件下产生了显著的电压和电流(P＜0.05);0.4% w/v KNN/GelMA组较0.2% w/v KNN/GelMA组的细胞增殖能力随时间显著增强,且活细胞 (绿色) 占主导地位(P＜0.05);RT-qPCR显示,TNF-α+KNN/GelMA+US组相比TNF-α+KNN/GelM组软骨细胞外基质中相关聚集蛋白聚糖(Acan)和Ⅱ型胶原蛋白(Col2)的 mRNA水平降低(P＜0.05)。结论构建了一种光固化可降解压电响应性水凝胶。其不仅展现出均匀的多孔结构、显著的压电效应,还具有良好的生物相容性和显著的促进髓核细胞细胞基质合成能力,为椎间盘退变损伤修复提供了潜在的优质材料选择。

关键词: 铌酸钾钠, 水凝胶, 压电效应, 椎间盘退变

Abstract: Objective To investigate the property of fundamental material properties of a piezoelectic-responsive hydrogel (KNN/GelMA), including its mechanical, piezoelectric and biocompatibility characteristics, and to examine the effects of its piezoelectric response the synthetic metabolism of nucleus pulposus cells (NPCs). Methods 1)Material characterisation: KNN was uniformly dispersed in GelMA hydrogel via ultrasonication to prepare a photopolymerisable, degradable piezoelectric hydrogel. Preliminary physicochemical characterisation was conducted by measuring microstructure, piezoelectric properties and so on. 2) Cellular experiments: The piezoelectic-responsive hydrogel was co-incubated with rat primary NPCs. The proliferation capacity and biocompatibility were assessed using the CCK-8 methods and a dead/live staining kit. Gene expression related to extra cellular matrix synthesis was detected by RT-qPCR. Results The material surface exhibited a uniform pore structure, with KNN nanoparticles distributed evenly throughout the hydrogel. Piezoelectric output experiments demonstrated significant voltage and current generation under applied force(P＜0.05). The proliferation of cells in the 0.4% w/v KNN/GelMA group was significantly enhanced over time as compared with that in 0.2% w/v KNN/GelMA group, and live cells (green) were dominant(P＜0.05). RT-qPCR showed that the mRNA levels of related aggrecan(Acan) and type Ⅱ collagen (Col2) in the extra cellular matrix of chondrocytes in the TNF-α + KNN/GelMA + ultrasound(US) group was lower than those in the TNF-α + KNN/GelM group(P＜0.05). Conclusions A light-curable, degradable, piezoelectric-responsive hydrogel which exhibits a uniform porous structure has developed. It has a significant piezoelectric effect, excellent biocompatibility, and can significantly promote the cell matrix synthesis ability of nucleus pulposus cells. It is a promising material option for repairing degenerative disc injuries.

Key words: potassium sodium niobate, hydrogel, piezoelectric effect, intervertebral disc degeneration

中图分类号:

R684.3

关云博, 赵子腾, 张自强, 孙笑非, 赵彦涛, 王祖强. 压电响应性水凝胶促进大鼠髓核细胞基质合成[J]. 基础医学与临床, 2026, 46(3): 325-331.

GUAN Yunbo, ZHAO Ziteng, ZHANG Ziqiang, SUN Xiaofei, ZHAO Yantao, WANG Zuqiang. Piezoelectric-responsive hydrogel promotes matrix synthesis in nucleus pulposus cells of rats[J]. Basic & Clinical Medicine, 2026, 46(3): 325-331.

参考文献

[1] GBD 2021 Low Back Pain Collaborators. Global, regional, and national burden of low back pain, 1990—2020, its attributable risk factors, and projections to 2050: a systematic analysis of the Global Burden of Disease Study 2021[J]. Lancet Rheumatol, 2023, 5: e316-e329.doi: 10.1016/S2665-9913(23)00098-X.
[2] Xin J, Wang Y, Zheng Z, et al. Treatment of intervertebral disc degeneration[J]. Orthop Surg, 2022, 14: 1271-1280. doi: 10.1111/os.13254.
[3] Mohd Isa IL, Teoh SL, Mohd Nor NH, et al. Discogenic low back pain: anatomy, pathophysiology and treatments of intervertebral disc degeneration[J]. Int J Mol Sci, 2022, 24:208. doi: 10.3390/ijms24010208.
[4] Chen Y, Li B, Xu Y, et al. Sal003 alleviated intervertebral disc degeneration by inhibiting apoptosis and extracellular matrix degradation through suppressing endoplasmic reticulum stress pathway in rats[J]. Front Pharmacol, 2023, 14: 1095307. doi: 10.3389/fphar.2023.1095307.
[5] Mahapatra SD, Mohapatra PC, Aria AI, et al. Piezoelec-tric materials for energy harvesting and sensing applications: roadmap for future smart materials[J]. Adv Sci (Weinh), 2021, 8: e2100864. doi: 10.1002/advs.202100864.
[6] Wu P, Xu C, Zou X, et al. Capacitive-coupling-responsive hydrogel scaffolds offering wireless in situ electrical stimulation promotes nerve regeneration[J]. Adv Mater, 2024, 36: e2310483. doi: 10.1002/adma.202310483.
[7] Liang J, Zeng H, Qiao L, et al. 3D printed piezoelectric wound dressing with dual piezoelectric response models for scar-prevention wound healing[J]. ACS Appl Mater Interfaces, 2022, 14: 30507-30522. doi: 10.1021/acsami.2c04168.
[8] Li G, Li Z, Min Y, et al. 3D-printed piezoelectric scaffolds with shape memory polymer for bone regeneration[J]. Small, 2023, 19: e2302927. doi: 10.1002/smll.202302927.
[9] Deng X, Zhuang Y, Cui J, et al. Open challenges and opportunities in piezoelectricity for tissue regeneration[J]. Adv Sci (Weinh), 2025, 12: e10349. doi: 10.1002/advs.202510349.
[10] Chen P, Xu C, Wu P, et al. Wirelessly powered electrical-stimulation based on biodegradable 3D piezoelectric scaffolds promotes the spinal cord injury repair[J]. ACS Nano, 2022, 16: 16513-16528. doi: 10.1021/acsnano.2c05818.
[11] Wang X, Liu Z, Zou L, et al. Size-optimized ceria nanoparticles attenuate intervertebral disc degeneration by rescuing nucleus pulposus cell senescence via reducing oxidative stress and stimulating PI3K/AKT pathway[J]. Mater Today Bio, 2025, 35: 102387. doi: 10.1016/j.mtbio.2025.102387.
[12] Yue K, Trujillo-de SG, Alvarez MM, et al. Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels[J]. Biomaterials, 2015, 73:254-271. doi:10.1016/j.biomaterials.2015.08.045.
[13] Shi M, Xu Q, Ding L, et al. Cell infiltrative inner connected porous hydrogel improves neural stem cell migration and differentiation for functional repair of spinal cord injury[J]. ACS Biomater Sci Eng, 2022, 8: 5307-5318. doi: 10.1021/acsbiomaterials.2c01127.
[14] Li H, Pan X, Wang T, et al. Piezoelectric nanomaterial-mediated physical signals regulate cell differentiation for regenerative medicine[J]. Small Sci, 2024, 4: 2300255. doi: 10.1002/smsc.202300255.

压电响应性水凝胶促进大鼠髓核细胞基质合成

Piezoelectric-responsive hydrogel promotes matrix synthesis in nucleus pulposus cells of rats

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 8

编辑推荐

Metrics

本文评价

[1]	陈旺, 李绪贵, 李瑛. 巨噬细胞极化影响腰椎间盘退变的研究进展[J]. 基础医学与临床, 2025, 45(9): 1229-1233.
[2]	李娜, 张鹏, 张楠, 林伟, 刘德峰, 郑继会. 灯盏花乙素减轻椎间盘退变模型大鼠髓核细胞凋亡[J]. 基础医学与临床, 2025, 45(9): 1158-1164.
[3]	刘德国, 陈宏, 张颉鸿, 郑宇翔, 刘振刚, 陈宣辰, 侯振海. 可注射水凝胶包埋骨髓间充质干细胞可有效促进损伤软骨的修复[J]. 基础医学与临床, 2025, 45(1): 44-50.
[4]	董军立, 蔡少康, 严蕾, 蔡毅. 二甲双胍抑制腰椎间盘退变模型兔椎间盘组织的细胞凋亡[J]. 基础医学与临床, 2023, 43(1): 116-122.
[5]	郝博雅, 曾菲, 温涛, 孟洁, 许海燕, 刘健. 咖啡酸改性明胶水凝胶制备及其抗氧化功能[J]. 基础医学与临床, 2022, 42(7): 1035-1041.
[6]	梁国新, 梁茵茹, 刘淑贤, 陈康振, 林勇, 秦江霞, 吴洪福. PF-127-LV-NTFs三维复合支架培养大鼠神经干细胞[J]. 基础医学与临床, 2022, 42(1): 75-81.
[7]	王赟, 徐宏宇, 潘宗友, 倪利承, 王刚祥, 赵勇, 郑强. 负载TGF-β3壳聚糖复合水凝胶促进兔半月板缺损修复[J]. 基础医学与临床, 2020, 40(11): 1512-1518.
[8]	陈春永沈正清叶君健. TWEAK和Fn14在腰椎间盘退变髓核组织中的表达及意义 [J]. 基础医学与临床, 2012, 32(9): 1053-1058.