衰老模型小鼠尿蛋白质组反映大脑皮质变化

doi:10.16352/j.issn.1001-6325.2026.03.0317

基础医学与临床 ›› 2026, Vol. 46 ›› Issue (3): 317-324.doi: 10.16352/j.issn.1001-6325.2026.03.0317

衰老模型小鼠尿蛋白质组反映大脑皮质变化

何梦真¹, 张玉雪¹, 刘婷婷¹, 胡斯奇², 李军², 孙伟^1*

1.中国医学科学院北京协和医学院基础医学研究所药理系,北京 100005;
2.中国人民解放军总医院第七医学中心儿科医学部研究所,北京 100010

收稿日期:2025-08-11 修回日期:2025-11-27 出版日期:2026-03-05 发布日期:2026-02-25
通讯作者: ^*sunwei@ibms.pumc.edu.cn
基金资助:
国家自然科学基金(82170524,31901039)

Urinary proteome in aging mouse models reflects changes in the cerebral cortex

HE Mengzhen¹, ZHANG Yuxue¹, LIU Tingting¹, HU Siqi², LI Jun², SUN Wei^1*

1. Department of Pharmacology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100005;
2. Faculty of Pediatrics, Senior of Department of Pediatrics, the Seventh Medical Center, General Hospital of Chinese PLA, Beijing 100010, China

Received:2025-08-11 Revised:2025-11-27 Online:2026-03-05 Published:2026-02-25
Contact: ^*sunwei@ibms.pumc.edu.cn

摘要/Abstract

摘要： 目的检测自然衰老小鼠大脑皮质和尿液的蛋白质组变化,并探索尿液蛋白质组与大脑皮质蛋白质组的关联。方法取1、6和18月龄小鼠各6只,收集尿液并分离生理条件下的大脑皮质。采用液相色谱-串联质谱(LC-MS/MS)技术进行蛋白质组分析,进行差异筛选和功能分析,研究尿液和大脑皮质蛋白质组的关联。结果在小鼠大脑皮质鉴定到13 021个蛋白质,在小鼠尿液鉴定到1 064个蛋白质。1月龄小鼠蛋白质组集中于生长、发育相关通路,6月龄小鼠蛋白质组集中于能量代谢等通路,18月龄小鼠蛋白质组表现出代谢异常和稳态失衡等特征。关联分析发现,尿液能够反映大脑皮质免疫、细胞增殖与衰老相关蛋白质的变化。结论衰老小鼠尿液蛋白质组可以反映大脑皮质变化,为衰老疾病标志物研究提供线索。

关键词: 衰老, 大脑皮质, 尿液, 蛋白质组学

Abstract: Objective To detect the proteomic changes in cerebral cortex and urine of aging mice and to explore the correlation between urinary and cerebral cortical proteomes. Methods Six mice of 1, 6 and 18-month-old were collec-ted, urine was collected and tissue was isolated from cerebral cortex under physiological conditions. Proteomic analysis was performed by liquid chromatography-tandem mass spectrometry(LC-MS/MS) was used to find potential differences and to analyze the function and the association between urine component and cerebral cortex proteomes. Results A total of 13 021 protein components were identified in the cerebral cortex and 1 064 protein components were identified in urine of mice. One-month-old mice proteomes focused on growth and development-related pathways, 6-month-old mice proteomes focused on energy metabolism and other pathways, and 18-month-old mice proteomes exhibited metabolic abnormalities and homeostatic imbalances. Correlation analysis revealed that urine can reflect the changes in proteins related to immunity, cell proliferation and aging in the cerebral cortex. Conclusions Urine proteome of senescent mice reflects cerebral cortex changes and provides clues for research on bio-markers of aging-related diseases.

Key words: aging, cerebral cortex, urine, proteomics

中图分类号:

R339.3+8

何梦真, 张玉雪, 刘婷婷, 胡斯奇, 李军, 孙伟. 衰老模型小鼠尿蛋白质组反映大脑皮质变化[J]. 基础医学与临床, 2026, 46(3): 317-324.

HE Mengzhen, ZHANG Yuxue, LIU Tingting, HU Siqi, LI Jun, SUN Wei. Urinary proteome in aging mouse models reflects changes in the cerebral cortex[J]. Basic & Clinical Medicine, 2026, 46(3): 317-324.

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衰老模型小鼠尿蛋白质组反映大脑皮质变化

Urinary proteome in aging mouse models reflects changes in the cerebral cortex

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