基础医学与临床 ›› 2022, Vol. 42 ›› Issue (5): 740-744.doi: 10.16352/j.issn.1001-6325.2022.05.004

• 研究论文 • 上一篇    下一篇

Fc-gamma Ⅰ型受体在阿尔茨海默病患者和模型小鼠脑皮质的表达

孙健茹, 仇文颖, 钱晓菁, 马超*   

  1. 中国医学科学院基础医学研究所 北京协和医学院基础学院 人体解剖与组织胚胎学系, 北京 100005
  • 收稿日期:2022-01-13 修回日期:2022-03-23 出版日期:2022-05-05 发布日期:2022-04-28
  • 通讯作者: * machao@ibms.cams.cn
  • 基金资助:
    国家自然科学基金(81771205);原创探索计划项目(82050004);中国医学科学院医学与健康科技创新工程项目(CIFMS #2021-1-I2M-025)

Expression of Fc-gamma receptor type Ⅰ in brain cortex of patients with Alzheimer's disease and the mouse model

SUN Jian-ru, QIU Wen-ying, QIAN Xiao-jing, MA Chao*   

  1. Department of Anatomy,Histology and Embryology,Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC,Beijing 100005,China
  • Received:2022-01-13 Revised:2022-03-23 Online:2022-05-05 Published:2022-04-28
  • Contact: * machao@ibms.cams.cn

摘要: 目的 探讨高亲和力IgG受体Fc-gamma Ⅰ型(FcγRⅠ)在阿尔茨海默病(AD)脑皮层的表达变化。方法 选用正常人脑组织样本和神经病理诊断为AD患者脑组织样本,6月龄野生型小鼠和APP/PS1小鼠。免疫荧光和免疫蛋白印迹检测脑皮层FcγRⅠ的细胞定位和表达。结果 人和小鼠脑皮层的神经元均普遍表达FcγRⅠ。与健康人脑相比,AD患者脑皮层的FcγRⅠ表达显著降低(P<0.05)。与野生型小鼠脑相比,AD模型小鼠脑皮层的FcγRⅠ表达也呈现出显著降低(P<0.05)。结论 IgG高亲和力受体FcγRⅠ在AD患者脑和AD模型小鼠脑皮层的表达显著下降,可能与AD的神经病理机制相关。

关键词: 阿尔茨海默病(AD), 神经元, 免疫球蛋白G(IgG), Fc-gammaⅠ型受体(FcγRⅠ)

Abstract: Objective To explore expression changes of high affinity IgG receptor Fc-gamma Ⅰ type(FcγRⅠ) in brain cortex of patients with Alzheimer's disease(AD). Methods Normal human brain tissue samples and brain tissue samples with neuropathological diagnosis of AD, wild type mice and APP/PS1 mice were used. Immunofluorescence and Western blot were used to define cellular localization and expression of FcγRⅠ in cerebral cortex. Results FcγRⅠ was generally expressed in neurons of human and mouse cerebral cortex. Compared with normal human brain, FcγRⅠ expression in AD human brain cortex decreased significantly(P<0.05). Compared with wild type mouse brain, FcγRⅠ expression in cerebral cortex of AD mouse models also decreased significantly(P<0.05). Conclusions The expression of IgG high affinity receptor FcγRⅠ in AD human brain and cerebral cortex of AD mouse models decreases significantly that suggestes that FcγRⅠ is potentially correlated with AD neuropathological pathogenesis.

Key words: Alzheimer's disease (AD), neuron, immunoglobulin G(IgG), Fc-gamma receptor type Ⅰ (FcγRⅠ)

中图分类号: