Effect of Vimentin expression by Regulated Nampt in glomerular cells in diabetic nephropathy
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Objective To study the function of endogenous Nampt on vimentin expression in glomerular cells. Methods With C57/BL6 diabetic mice experiment, the renal pathological tissue bebing embedded, fixated and sliced, endogenous Nampt and vimentin expression and location are analysized by appling confocal microscope technique. After 5 days in cultured with 200 mmol/L high concentration glucose (hyperglucose), HBZY-1 cells intervened with 10 μmol/L FK866 and 1 mmol/L NMN, Nampt, NF-κB, Sirt1 and vimentin are measured by immunofluorescence and Western blot assay respectively. HBZY-1 cells were divided into groups (24 h,48 h,72 h,96 h,120 h and 144 h) after cultured with hyperglucose, The time-effect course on expression of endogenous Nampt, NF-κB and Sirt1are measured by Western blot assay. Results 1) With glomerular atrophy, excess vimentin expression clearly increased along with Nampt high expression in DN glomerular cells, as compared with the control group (P﹤0.01). 2) After the cells treated with FK866, vimentin expression decreased significantly following Nampt expression inhibited (P﹤0.01). With NMN intervention, vimentin expression is very lower than that of control group (P﹤0.01). 3) With the extension of incubation time of the in the HBZY-1 cells in high concentrations glucose, the expression of Nampt and NF-κB obviously increased (P﹤0.01), while the SIRT1 expression decreased significantly, as compared with control groups (p﹤0.01). Conclusion In DN glomerular fibrosis, Nampt can control vimentin expression by enhenced NF-κB and inhibited SIRT1signaling pathway.