Down-regulation of BECN1 inhibits the proliferation, invasion and migration of human leukemia cell line THP-1

doi:10.16352/j.issn.1001-6325.2023.07.1104

Abstract

Abstract: Objective To analyze the effect of down-regulation of BECN1 on the proliferation,invasion and migration of human leukemia cell line THP-1,and to explore its potential molecular mechanism. Methods The lentivirus expressing shRNA targeting at BECN1 and the negative control were constructed by RNA interference. THP-1 cells were divided into control group(control),negative control group(NC) and BECN1 interference group (BECN1-shRNA). Stable cell strains were selected by puromycin after infection by lentivirus. The down-regulation effect of BECN1 was identified by RT-qPCR and Western blot. MTT and Transwell assays were used respectively to detect the proliferation,invasion and migration of THP-1 cells. The expression of MMP 2 and MMP 9 were detected by Western blot. Results THP-1 stable cell strain expressing GFP was obtained by puromycin selection. Compared with the control and negative control group, the BECN1 mRNA and protein expression in the BECN1-shRNA group were significantly decreased (P<0.05); The proliferation,invasion and migration of cells also decreased significantly(P<0.05);The expression of MMP 2 and MMP 9 decreased (P<0.05). Conclusions Down-regulation of BECN1 may inhibit the proliferation,invasion and migration of THP-1 cells,and its molecular mechanism is related to the down-regulation of MMP 2 and MMP 9.

Key words: BECN1, leukemia, proliferation, invasion, migration

CLC Number:

R733.71

ZENG Yankun, HONG Xiaoying, CHEN Qinglin. Down-regulation of BECN1 inhibits the proliferation, invasion and migration of human leukemia cell line THP-1[J]. Basic & Clinical Medicine, 2023, 43(7): 1104-1109.

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URL: http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/10.16352/j.issn.1001-6325.2023.07.1104

http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2023/V43/I7/1104

References

[1] Hu F,Li G,Huang C,et al. The autophagy-independent role of BECN1 in colorectal cancer metastasis through regulating STAT3 signaling pathway activation[J]. Cell Death Dis,2020,11:304-317.
[2] 张玉领, 李春梅, 陈培,等. 体外转染BECN1对MG803胃癌细胞自噬及生长的影响[J]. 中国老年学杂志, 2019, 39:3486-3488.
[3] Hamurcu Z,Deliba?i N,Geçene S,et al. Targeting LC3 and Beclin-1 autophagy genes suppresses proliferation,survival,migration and invasion by inhibition of Cyclin-D1 and uPAR/Integrin β1/Src signaling in triple negative breast cancer cells[J]. Cancer Res Clin Oncol,2018,144:415-430.
[4] Kessenbrock K,Plaks V,Werb Z.Matrix metalloproteinases:regulators of the tumor microenvironment[J]. Cell,2010,141:52-67.
[5] Kapoor C, Vaidya S, Wadhwan V,et al. Seesaw of matrix metalloproteinases (MMPs)[J]. Cancer Res Ther,2016,12:28-35.
[6] Kim TD,Rea D,Schwarz M,et al. Peripheral artery occlusive disease in chronic phase chronic myeloid leukemia patients treated with nilotinib or imatinib[J]. Leukemia,2013,27:1316-1321.
[7] 邓怡,张曦. 白血病中钙黏蛋白的表观遗传学调控[J]. 检验医学与临床, 2017,14:1348-1351.
[8] Mowers EE,Sharifi MN,Macleod KF.Autophagy in cancer metastasis[J]. Oncogene,2017,36:1619-1630.
[9] Yu C,Gorantla SP,Müller-Rudorf A,et al. Phosphory-lation of BECLIN-1 by BCR-ABL suppresses autophagy in chronic myeloid leukemia[J]. Haematologica,2020,105:1285-1293.
[10] 韩晨阳,杨毅,郭丽,等. 自噬基因beclin 1抑制后增强人髓性白血病耐药细胞K562/IMA对于伊马替尼的药物敏感性[J]. 中国药学杂志,2019,54:284-290.
[11] Principe M, Buccisano F, Soddu S, et al. Involvement of central nervous system in adult patients with acute myeloid leukemia: inoidence and impact on outcome[J]. Semin Hematol, 2018,55:209-214.
[12] 覃凤娴,邵会媛,陈先春,等. 过表达突变的NPM1基因对THP-1白血病细胞增殖和凋亡的影响[J]. 基础医学与临床,2012,32:251-256.
[13] Si MY,Fan ZC,Li YZ,et al. The prognostic significance of serum and cerebrospinal fluid MMP-9,CCL2 and sVCAM-1 in leukemia CNS metastasis[J]. Neurooncol,2015,122:229-244.
[14] Chen YJ,Chang LS.Simvastatin induces NFκB/p65 down-regulation and JNK1/c-Jun/ATF-2 activation,leading to matrix metalloproteinase-9 (MMP-9) but not MMP-2 down-regulation in human leukemia cells[J]. Biochem Pharmacol,2014,92:530-543.

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