Abstract: Objective To investigate the effect and possible mechanism of sorafenib on the proliferation, migration and invasion of breast cancer cell line T47D. Methods Effects of sorafenib (2.5, 5 and 10 μmol/L) on T47D cells proliferation, colony formation, migration and invasion were detected by CCK-8 experiment, plate cloning experiment, scratch healing experiment and Transwell experiment. RT-qPCR was applied to detect circ_0032821 expression. The circ_0032821 small interfering RNA(si-circ_0032821) and circ_0032821 over-expression plasmid were transfected into T47D cells respectively. And the effect of interference with circ_0032821 or circ_0032821 over-expression combined with sorafenib on the malignant behavior of T47D cells was detected. Results The cell proliferation inhibition rate T47D cells were significantly increased after sorafenib treatment (P＜0.05); The colony formation number, scratch healing rate, invasion number, and circ_0032821 expression were significantly decreased(P＜0.05).After silencing with the expression of circ_0032821, the proliferation inhibition rate of T47D cells was significantly increased (P＜0.05); The colony formation number, scratch healing rate and invasion number were significantly reduced(P＜0.05). Circ_0032821 over-expression significantly reduced the effects of sorafenib on the proliferation, migration colony formation and invasion of T47D cells (P＜0.05). Conclusions Sorafenib can inhibit T47D cell proliferation, migration and invasion. And the mechanism may be explained by the down-regulation of circ_0032821.

Key words: sorafenib, breast cancer, proliferation, migration, circ_0032821