Imaging of breast cancer targeted by the nucleic acid aptamer W3-quantum dots

doi:10.16352/j.issn.1001-6325.2023.01.0076

Abstract

Abstract: Objective To perform quantum dots(QDs)imaging of breast cancer cells and breast cancer tissue paraffin specimens by using nucleic acid aptamer W3 binding specificity on breast cancer cells. Methods The binding specificity of nucleic acid aptamer W3 to breast cancer cell lines was detected by flow cytometry, and based on the binding reaction principle of biotin and streptomyces avidin, the quantum dot targeting imaging of nucleic acid aptamer W3 on breast cancer cell lines and tissue specimens of clinical patients was observed by fluorescence microscopy, and its clinical significance was further analyzed. Results The nucleic acid aptamer W3 specifically binded to breast cancer cell lines with high malignancy,and after coupling quantum dots QD605(W3-QDs), it achieved targeted imaging of breast cancer cell lines and breast cancer tissues with high malignancy, and the fluorescence intensity of breast cancer tissues with high malignancy degree was significantly higher than that of breast cancer tissues with low malignancy. Conclusions The nucleic acid aptamer W3 may achieve target imaging of breast cancer after coupling quantum dots, which can be used for early diagnosis of breast cancer.

Key words: aptamer, breast cancer, quantum dot, targeted imaging

CLC Number:

WANG Qun, LI Xin, MA Jun, LI Wanming. Imaging of breast cancer targeted by the nucleic acid aptamer W3-quantum dots[J]. Basic & Clinical Medicine, 2023, 43(1): 76-82.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/10.16352/j.issn.1001-6325.2023.01.0076

http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2023/V43/I1/76

References

[1] Harbeck N, Gnant M. Breast cancer[J]. Lancet, 2017, 389:1134-1150.
[2] Conti A, Duggento A, Indovina I, et al. Radiomics in breast cancer classification and prediction[J]. Semin Cancer Biol, 2021, 72:238-250.
[3] 李晓欧, 胡燕, 安雅聪, 等.针对于白蛋白的新型核酸适配体的筛选与鉴定[J]. 基础医学与临床, 2017, 37:935-938.
[4] Wang S, Li W, Yuan D, et al. Quantitative detection of the tumor-associated antigen large external antigen in colorectal cancer tissues and cells using quantum dot probe[J]. Int J Nanomedicine, 2016, 11:235-247.
[5] Liang Z, Khawar MB, Liang J, et al. Bio-Conjugated quantum dots for cancer research: detection and imaging[J]. Front Oncol, 2021, 11:749970. doi:10.3389/fonc.2021.749970.
[6] Matea CT, Mocan T, Tabaran F, et al. Quantum dots in imaging, drug delivery and sensor applications[J]. Int J Nanomedicine, 2017, 12:5421-5431.
[7] Li WM, Bing T, Wei JY, et al. Cell-SELEX-based selection of aptamers that recognize distinct targets on metastatic colorectal cancer cells[J]. Biomaterials, 2014, 35:6998-7007.
[8] Bauer M, Strom M, Hammond DS, et al. Anything you can do, I can do better: Can aptamers replace antibodies in clinical diagnostic applications?[J]. Molecules, 2019, 24:4377. doi:10.3390/molecules24234377.
[9] Ni S, Zhuo Z, Pan Y, et al. Recent progress in aptamer discoveries and modifications for therapeutic applications[J]. ACS Appl Mater Interfaces, 2021, 13:9500-9519.
[10] Zeng Z, Zhang P, Zhao N, et al. Using oligonucleotide aptamer probes for immunostaining of formalin-fixed and paraffin-embedded tissues[J]. Mod Pathol, 2010, 23: 1553-1558.
[11] Kundu S, Ghosh M, Sarkar N. State of the art and perspectives on the biofunctionalization of fluorescent metal nanoclusters and carbon quantum dots for targeted imaging and drug delivery[J]. Langmuir, 2021, 37:9281-9301.
[12] Leonel AG, Mansur AAP, Mansur HS. Advanced functional nanostructures based on magnetic iron oxide nanomaterials for water remediation: a review[J]. Water Res, 2021, 190:116693. doi:10.1016/j.watres.2020.116693.
[13] Díaz-González M, Escosura-Muñiz A, Fernandez-Argüelles MT, et al. Quantum dot bioconjugates for diagnostic applications[J]. Top Curr Chem, 2020, 378:35. doi:10.1007/s41061-020-0296-6.
[14] Zhao T, Fu Y, Jang MS, et al. A pH-activated charge convertible quantum dot as a novel nanocarrier for targeted protein delivery and real-time cancer cell imaging [J]. Mater Sci Eng C Mater Biol Appl, 2021, 118:111449. doi:10.1016/j.msec.2020.111449.

[1]	YANG Liangquan, YU Miao, JIANG Qian, ZHANG Minghui, YANG Haisong. miR-504 regulates proteasome activator and inhibits the proliferation and migration of breast cancer cell line MCF-7 [J]. Basic & Clinical Medicine, 2023, 43(1): 95-101.
[2]	ZHANG Xiao-lei, LIU Jing, LI Chao-ping. Circ_0032821 is involved in sorafenib's inhibition of the proliferation, migration and invasion of breast cancer cell line T47D [J]. Basic & Clinical Medicine, 2022, 42(9): 1350-1354.
[3]	WANG Zi-yuan, DUAN Zhao-jun, LUO Yun-ping. Establishment and validation of lung-specific metastatic cell subline of mouse breast cancer [J]. Basic & Clinical Medicine, 2022, 42(7): 1083-1091.
[4]	HAN Xue-ying, CUI Feng, LI Jun. Deleted in breast cancer 1(DBC1) affects the sensitivity of breast cancer cell lines to etoposide [J]. Basic & Clinical Medicine, 2022, 42(7): 1060-1066.
[5]	KANG You-li, ZHAO Dan, DU Wen-jing, LI Li. SIRT5 promotes the proliferation of human breast cancer cells by regulating the succinylation of ALDH5A1 [J]. Basic & Clinical Medicine, 2022, 42(7): 1099-1107.
[6]	XIE Li-hua, YANG Qi, LI Ya-qian, XIE Xin-yue, LEI Shi-juan, ZHOU Lin. Transcription factor Kaiso down-regulates the expression of lncRNA MEG3 in human breast cancer cell lines [J]. Basic & Clinical Medicine, 2022, 42(5): 758-762.
[7]	HE Xiao, HU Wei-jie, LYU Wen-chang, REN Yu-ping, WU Min, WU Yi-ping, ZHANG Qi. Advances on the roles of circular RNA in breast cancer [J]. Basic & Clinical Medicine, 2022, 42(3): 507-511.
[8]	SHAO Zhi-ding, GAO Xue, REN Jing, TANG Xiao-lei. Screening aptamers for 5-hydroxytryptamine receptor and its anti-depressant function [J]. Basic & Clinical Medicine, 2022, 42(10): 1514-1521.
[9]	ZHANG Yan, ZHU Meng-wen, LIU Tong, YANG Sheng, LIANG Ge-yu. Research progress on the role of N6-methyladenosine (m6A) RNA modification in reproductive system diseases [J]. Basic & Clinical Medicine, 2021, 41(9): 1347-1351.
[10]	XIONG Yu-yuan, CHEN De-jie, MENG Lin, YAN Shan. TLK2 promotes the proliferation, migration and invasion of human breast cancer cell lines [J]. Basic & Clinical Medicine, 2021, 41(8): 1156-1162.
[11]	WANG Hai-yan, HUANG Ming-quan, PAN Guang-rui, WANG Gui-lin, LIANG Bin, QUAN Yi. Hypoxia microenvironment promotes invasion and proliferation of human breast cancer cell line MCF-7 [J]. Basic & Clinical Medicine, 2021, 41(4): 539-544.
[12]	ZHENG Ya-nan, LI Mei, ZHAO Ji-yuan. Progress in molecular mechanism and targeted drugs of bone metastases in breast cancer [J]. Basic & Clinical Medicine, 2021, 41(3): 433-437.
[13]	ZHANG Tian-rui, GAO Wen-yi, YAO Juan. Effects of KDM3A silencing on the apoptosis and invasion of human breast cancer cell line MDA-MB-231 [J]. Basic & Clinical Medicine, 2021, 41(1): 33-37.
[14]	YANG De-quan, LI Chang-you, CUI Jun-wei, WEI Wei, WANG Shao-ping. hsa-miR-4483 promotes the proliferation and migration of breast cancer cell lines [J]. Basic & Clinical Medicine, 2020, 40(9): 1230-1236.
[15]	HU Qing-qing, LU Yan. Indoleamine 2,3-dioxygenase 1 inhibitor Epacadostat combined with IFN-γ induces apoptosis of triple-negative breast cancer cell lines [J]. Basic & Clinical Medicine, 2020, 40(5): 615-620.