关键词: 食管癌, 细胞增殖, 细胞凋亡, MK886, 脂氧合酶

Abstract: Objective To investigate the effects of MK886,a 5-LOX-activating protein(FLAP)inhibitor on the proliferation and apoptosis of human esophageal carcinoma cells KYSE-150 and TE-3,and to explore the possible mechanism.Methods KYSE-150 and TE-3 cells were treated with different concentrations of MK886(2.5,5,10,20,40 and 80μmol/L).Used xCELLigence RTCA monitor real-time the growth of cells to detect the half maximal inhibitory concentration(IC50)of MK886 in various cells.After the treatment with different concentrations of MK886,the expression levels of proteins with apoptosis and autophagy were detected by Western blotting,and cell cycle were detected by flow cytometry.Result MK886 decreased the proliferation of human esophageal carcinoma cells KYSE-150 and TE-3 (both P＜0.05).The IC50 values of MK886 in KYSE-150 is 29.11μmol/L,in TE-3 is 27.47μmol /L.The proportion of KYSE-150 and TE-3 cells treated with concentrations of MK-886 25μmol/L,G0/G1 phase were increased（both P＜0.001）however treated with concentrations of MK-886 50μmol/L,G2/M phase were increased(both P＜0.005). Did not detected the proteins expression levels of PARP, caspase-9 were increased with different concentrations of MK886.The expression levels of cleaved Caspase-3 and LC-3A/B-Ⅱwere detected increased with MK886 (both P＜0.001).Conclusion MK886 can inhibit the proliferation of KYSE-150 and TE-3 cells induce apoptosis and cell cycle,however autophagy induced by high concentrations MK886,provided the theoretical reference for clinical medication.

Key words: esophageal carcinoma, cell proliferation, apoptosis, MK886, Lipoxygenase