hsa-miR-4483促进人乳腺癌细胞系的增殖和迁移

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (9): 1230-1236.

hsa-miR-4483促进人乳腺癌细胞系的增殖和迁移

阳德全¹, 李常有², 崔军威², 韦伟², 王少平^1*

1.中山大学附属第八医院肝胆外科, 广东深圳 518000;
2.北京大学深圳医院甲乳外科,广东深圳 518000

收稿日期:2019-08-26 修回日期:2019-12-06 出版日期:2020-09-05 发布日期:2020-09-04
通讯作者: ^*snakewsp@163.com
基金资助:
深圳市科技项目(JCYJ20170307111713483)

hsa-miR-4483 promotes the proliferation and migration of breast cancer cell lines

YANG De-quan¹, LI Chang-you², CUI Jun-wei², WEI Wei², WANG Shao-ping^1*

1. Department of Hepatobiliary Surgery, the Eighth Affiliated Hospital,Sun Yat-sen University,Shenzhen 518000;
2. Department of Thyroid and Breast Surgery, Peking University Shenzhen Hospital,Shenzhen 518000, China

Received:2019-08-26 Revised:2019-12-06 Online:2020-09-05 Published:2020-09-04
Contact: ^*snakewsp@163.com

摘要/Abstract

摘要： 目的探讨hsa-miR-4483在乳腺癌细胞系中表达及作用。方法利用TargetScan找出hsa-miR-4483可作用c-Myc基因;利用RT-qPCR对hsa-miR-4483的表达量进行验证。转染MCF-7和MDA-MB-231,并设置NC阴性对照,Western blot检测hsa-miR-4483作用c-myc基因的表达量,利用MTT法和Transwell小室法验证乳腺癌细胞的增殖及迁移的能力。结果 hsa-miR-4483在MCF-7和MDA-MB-231中显著低表达(P＜0.001,P＜0.01);MCF-7和MDA-MB-231的c-myc蛋白表达量上调(P＜0.05);hsa-miR-4483促进了MCF-7和MDA-MB-231细胞增殖和迁移(P＜0.001)。结论 hsa-miR-4483可促进了乳腺癌细胞系的增殖以及迁移,其在乳腺癌的发展及其预后中起到调控的作用。

关键词: microRNA, 乳腺癌, 增殖, 迁移, 增殖曲线

Abstract: Objective To explore the expression and function of hsa-miR-4483 in breast cancer cells. Methods Using TargetScan to find out how can-miR-4483 act on the c-Myc gene, and RT-qPCR verified the expression of hsa-miR-4483. Transfected MCF-7 and MDA-MB-231 and set up NC-negative controls, Western blot was used to detect the expression of the hsa-miR-4483 gene, MTT assay and Transwell chamber experiment were applied to verify the proliferation and migration of breast cancer cells. Results The expression of hsa-miR-4483 was significantly lower (P＜0.001, P＜0.01) in MCF-7 and MDA-MB-231(P＜0.001, P＜0.01) The up-regulation of c-myc in MCF-7 and MDA-MB-231 promoted cell proliferation and migration (P＜0.001). Conclusions hsa-miR-4483 can promote the proliferation and migration of breast cancer cell lines, which plays a regulatory role in the development of breast cancer and its prognosis.

Key words: microRNA, breast cancer, proliferation, migration, proliferation curve

中图分类号:

R655.8

阳德全, 李常有, 崔军威, 韦伟, 王少平. hsa-miR-4483促进人乳腺癌细胞系的增殖和迁移[J]. 基础医学与临床, 2020, 40(9): 1230-1236.

YANG De-quan, LI Chang-you, CUI Jun-wei, WEI Wei, WANG Shao-ping. hsa-miR-4483 promotes the proliferation and migration of breast cancer cell lines[J]. Basic & Clinical Medicine, 2020, 40(9): 1230-1236.

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