Lunasin减轻大鼠运动性关节软骨损伤

基础医学与临床 ›› 2017, Vol. 37 ›› Issue (5): 691-695.

Lunasin减轻大鼠运动性关节软骨损伤

刘粟¹,邱勇²,刘红²,贾绍辉³,刘万红⁴

1. 湖北民族学院科技学院
2. 湖北民族学院
3. 武汉体育学院
4. 武汉大学基础医学院

收稿日期:2016-10-20 修回日期:2017-01-22 出版日期:2017-05-05 发布日期:2017-04-19
通讯作者: 贾绍辉 E-mail:479275148@qq.com
基金资助:
国家体育总局科研项目

Lunasin alleviates the sports articular cartilage injury of rats

Received:2016-10-20 Revised:2017-01-22 Online:2017-05-05 Published:2017-04-19

摘要/Abstract

摘要： 目的探讨Lunasin对运动性关节软骨损伤的治疗作用及机制。方法将大鼠随机分为对照组和模型组，在注射给予木瓜蛋白酶造模后，标准饲养28 d，然后将模型大鼠分为单纯模型组、0.01、0.05和0.1 mmol/L Lunasin治疗组（每组10只，治疗时间1个月）。治疗结束后分别用ELISA和RT-PCR方法检测血清及组织中IL-1β、IL-6、TNF-α、MMP-6及MMP-8 mRNA的表达；用试剂盒检测SOD活性及iNOS，用Western Blot检测NRF2、Keap1、LC-3Ⅱ、Bax、Beclin1、p-AMPK和AMPK蛋白的表达。结果模型组中IL-1β、IL-6、TNF-α、MMP-6以及MMP-8在血清及组织中含量较对照组均显著升高（P < 0.05），Lunasin治疗后上述因子均显著回降（P < 0.05）；并且Lunasin治疗能显著提高SOD的活性（P < 0.05），上调NRF2的表达并降低iNOS的产生（P < 0.05）；另外，Lunasin能够上调受损关节软骨组织中自噬蛋白Beclin1以及LC-3Ⅱ的表达，抑制软骨细胞的凋亡蛋白Bax的表达。结论 Lunasin能够通过降低炎性介质的产生、激活氧化应激系统和自噬通路从而有利于受损关节的修复。

关键词: Lunasin, 凋亡, 自噬, 运动性关节软骨损伤

Abstract: Objective To exploer the function and underlying mechanism of Lunasin on sports articular cartilage injury. Methods Wistar rats were randomly divided into negative control group and model group; after injection molding for 3 times, the model rats were feed for 28 days. Then the model rats were divided into sham model group, 0.01, 0.05 and 0.1 mmol/L Lunasin treatment group respectively. After treatment, ELISA was used to analyze the production of IL-1β、IL-6、TNF-α、MMP-6 and MMP-8. The SOD activity and iNOS were evaluated by their ELISA kit. Western blot was used to detect the expression of NRF2, Keap1, LC-3Ⅱ, Bax, Beclin1, p-AMPK, AMPK. Results Compared with control, the dose of IL-1β、IL-6、TNF-α、MMP-6 and MMP-8 in serum of model rats were significantly increased (P < 0.05), however, after treatment with lunasin for 1 month, these inflammatory factors were obviously reduced then that of model rats (P < 0.05); Furthermore Lunasin treatment obviously increase SOD activity、up-regulate NRF2 expression and down-regulate the generation of nitric oxide synthase (iNOS) (P < 0.05); Additionally, Lunasin can raise the expression of autophagy-related protein(beclin1 and LC-3Ⅱ), reduce the expression of apoptosis protein (Bax) in damaged articular cartilage. Conclusion Lunasin benefit the repair of damaged joints by reducing the production of inflammatory mediators, activating of oxidative stress system and autophagy pathway.

Key words: Luansin, apoptosis, autophagy, sports articular cartilage injury

刘粟邱勇刘红贾绍辉刘万红. Lunasin减轻大鼠运动性关节软骨损伤[J]. 基础医学与临床, 2017, 37(5): 691-695.

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