S-腺苷同型半胱氨酸对大鼠主动脉内皮细胞增殖及凋亡的影响

基础医学与临床 ›› 2010, Vol. 30 ›› Issue (10): 1037-1040.

S-腺苷同型半胱氨酸对大鼠主动脉内皮细胞增殖及凋亡的影响

欧三桃柳飞刘琦张帆侯静温向琼

泸州医学院附属医院四川大学华西医院泸州医学院附属医院泸州医学院附属医院泸州医学院附属医院泸州医学院附属医院

收稿日期:2009-09-01 修回日期:2010-01-05 出版日期:2010-10-05 发布日期:2010-10-05
通讯作者: 欧三桃

Effects of S-adenosylhomocysteine on proliferation and apoptosis of rat SV40-transformed aortic rat endothelial cell

San-tao OU, Fei LIU, Qi LIU, Fan ZHANG, Jing HOU, Xiang-qiong WEN

Affiliated Hospital of Luzhou Medical College West China Hospital of Sichuan University

Received:2009-09-01 Revised:2010-01-05 Online:2010-10-05 Published:2010-10-05
Contact: San-tao OU,

摘要/Abstract

摘要： 目的研究S-腺苷同型半胱氨酸（AdoHcy）对大鼠主动脉内皮细胞增殖和凋亡的影响，以及对细胞培养上清液中VCAM-1含量的影响。方法将不同浓度的AdoHcy水解酶抑制剂3-deazaadenosine（DZA）作用于鼠主动脉内皮细胞(SVAREC) 24、48和72 h后，荧光偏振免疫分析法测定同型半胱氨酸（Hcy）浓度，反相高效液相色谱法测定AdoHcy浓度，WST-1法检测细胞增殖，TUNEL法检测细胞凋亡，ELISA法检测细胞培养上清液VCAM-1含量。结果 SVAREC经不同浓度DZA处理后，Hcy浓度降低而细胞内AdoHcy浓度上升，细胞增殖能力下降并出现凋亡，细胞培养上清液中VCAM-1含量升高。结论 AdoHcy可抑制SVAREC增殖，促进其凋亡及诱导炎性因子VCAM-1的表达，从而致主动脉内皮细胞损伤，参与动脉粥样硬化的形成。

关键词: S-腺苷同型半胱氨酸, 血管内皮细胞, 凋亡, VCAM-1

Abstract: Objective To study the effect of AdoHcy on proliferation and apoptosis of SVAREC and the content of VCAM-1 in the supernatant of SVAREC culture.Methods After SVAREC were treated with different concentrations of 3-deazaadenosine (DZA) for 24, 48 and 72h, the cellular AdoHcy level and the homocysteine (Hcy) were measured with different methods The cell growth and proliferation were determined by WST-1 assay. The percentage was analyzed with TUNEL methods. The content of VCAM-1 in the supernatant of SVAREC culture was analyzed with ELISA methods Results After SVAREC were treated with different concentrations of DZA, the content of Hcy decreased while the intracellular AdoHcy level increased.Compared with the control group, AdoHcy could inhibit the proliferation and induce apoptosis of SVAREC in a dose-dependent way and induce secretion of VCAM-1. Conclusions AdoHcy could inhibit the proliferation and induce apoptosis of SVAREC, induce secretion of VCAM-1.which may be one of mechanisms responsible for injury of aorta endothelial cell lead to atherosclerosis.

Key words: S-adenosylhomocysteine, vascular endothelial cell, apoptosis, VCAM-1

欧三桃柳飞刘琦张帆侯静温向琼. S-腺苷同型半胱氨酸对大鼠主动脉内皮细胞增殖及凋亡的影响[J]. 基础医学与临床, 2010, 30(10): 1037-1040.

San-tao OU; Fei LIU; Qi LIU; Fan ZHANG; Jing HOU; Xiang-qiong WEN. Effects of S-adenosylhomocysteine on proliferation and apoptosis of rat SV40-transformed aortic rat endothelial cell[J]. Basic & Clinical Medicine, 2010, 30(10): 1037-1040.

[1]	刘雯霞刘彩红郭蕊孟芝君高佳王亚静. 尼古丁促进高糖/高脂培养的大鼠心肌细胞系H9C2凋亡[J]. , 2020, 40(1): 24-29.
[2]	蒋庆锋于永魁程金华申思宁邢文群. lncRNA HEIH通过miR-98-5p调节肺癌细胞增殖和凋亡[J]. , 2020, 40(1): 30-36.
[3]	苗玉娟王淑坤孙丹丁康刘美逸张妍周风华. miR-181 通过 Prox1 促进人乳腺癌细胞系MCF-7的增殖并抑制凋亡[J]. 基础医学与临床, 2019, 39(9): 1265-1269.
[4]	童静凯江霞. 托伐普坦对小鼠内髓集合管细胞系水通道蛋白及肾小管功能的影响[J]. 基础医学与临床, 2019, 39(8): 1163-1167.
[5]	晋瑞金迎迎李嫚雷喆. 过表达脾酪氨酸激酶降低氧化应激诱导的小鼠成骨细胞凋亡[J]. 基础医学与临床, 2019, 39(7): 1013-1018.
[6]	任玮余宏川王萍刘英茹. miR-17-5p诱导小鼠肾足细胞系凋亡[J]. 基础医学与临床, 2019, 39(7): 973-977.
[7]	雷晓燕孙永红陈星星崔维静刘璟. 乙肝病毒X蛋白抑制小鼠足细胞系MPC5增殖并促进其凋亡[J]. 基础医学与临床, 2019, 39(5): 617-622.
[8]	马从乾王雅王娜杨柯陈德才. miR-181a过表达加重H2O2诱导的HUVECs损伤[J]. 基础医学与临床, 2019, 39(5): 690-695.
[9]	王圣凯胡苏琴吴玲吴杭菲郑靖宇蒋自培李剑敏. miR-138在乙醇诱导的大鼠心肌细胞系H9C2氧化应激中表达增加[J]. 基础医学与临床, 2019, 39(5): 677-681.
[10]	战仕胜李军杨婷婷王雅蓉王秀青. 抗菌肽merecidin诱导人肺腺癌细胞系A549凋亡[J]. 基础医学与临床, 2019, 39(4): 473-477.
[11]	刘莹张艳丽王秀芳. 沉默FOXO1抑制H2O2诱导的大鼠肺泡2型上皮细胞凋亡[J]. 基础医学与临床, 2019, 39(4): 519-523.
[12]	张娟冯燕和水祥. 过表达miR-5195-3p对结直肠癌细胞凋亡的影响[J]. 基础医学与临床, 2019, 39(3): 385-391.
[13]	张许梅王瑾霍海燕岳继萍张文婷焦向英. 血管紧张素II 1型受体自身抗体可上调TXNIP导致大鼠胰岛β细胞系INS-1凋亡[J]. , 2019, 39(11): 1548-1555.
[14]	宁娜侯晓鸿李杨何金玲燕子王晓晖王丽. 自噬降低激活β1-AA诱导的心肌细胞内质网应激相关凋亡途径[J]. 基础医学与临床, 2019, 39(1): 1-6.
[15]	赵承承陈建泉吕纯业. 转染miRNAs降低脂多糖诱导的大鼠肠微血管内皮细胞水通道蛋白1表达[J]. 基础医学与临床, 2019, 39(1): 42-46.