人羊膜间充质干细胞对大鼠子宫瘢痕的修复作用

doi:10.16352/j.issn.1001-6325.2024.07.1002

基础医学与临床 ›› 2024, Vol. 44 ›› Issue (7): 1002-1007.doi: 10.16352/j.issn.1001-6325.2024.07.1002

人羊膜间充质干细胞对大鼠子宫瘢痕的修复作用

宋佳¹, 赵峰², 张婷³, 徐静¹, 孙静莉¹, 陈震宇^1*

1.北部战区总医院妇产科,辽宁沈阳 110016;
2.中国医科大学干细胞与再生医学研究室,辽宁沈阳 110122;
3.锦州医科大学妇产科学系,辽宁锦州 121001

收稿日期:2024-01-10 修回日期:2024-03-29 出版日期:2024-07-05 发布日期:2024-06-26
通讯作者: ^*czy740704@163.com
基金资助:
辽宁省科学技术计划(2022JH2,101500029)

Repair effect of human amniotic mesenchymal stem cells on uterine scars in rats

SONG Jia¹, ZHAO Feng², ZHANG Ting³, XU Jing¹, SUN Jingli¹, CHEN Zhenyu^1*

1. Department of Obstetrics and Gynecology, General Hospital of Northern Theater Command, Shenyang 110016;
2. Department of Stem Cells and Regenerative Medicine,China Medical University,Shenyang 110122;
3. Department of Obstetrics and Gynecology,Jinzhou Medical University,Jinzhou 121001, China

Received:2024-01-10 Revised:2024-03-29 Online:2024-07-05 Published:2024-06-26
Contact: ^*czy740704@163.com

摘要/Abstract

摘要： 目的探究人羊膜间充质干细胞(hAMSCs)对大鼠子宫瘢痕的修复作用。方法分离培养hAMSCs,选雌性SPF级SD大鼠行子宫壁全层切开术,术中注射hAMSCs,于术后第30天对子宫切开部位进行组织学检查。用ImageJ图像分析软件进行分析比较各组子宫肌层厚度、纤维化面积百分比。免疫组化法分别检测子宫肌层α平滑肌肌动蛋白(α-SMA)、转化生长因子β1(TGF-β1)、肿瘤增殖抗原Ki-67(Ki-67)阳性面积百分比。结果与磷酸缓冲盐溶液(PBS)组相比,hAMSCs组子宫肌层明显增厚、纤维化面积减少,α-SMA、Ki-67阳性表达明显增加(P＜0.05),而TGF-β1阳性表达明显减少(P＜0.05)。结论人羊膜间充质干细胞(hAMSCs)可能通过减少瘢痕纤维化的形成、促进子宫瘢痕处平滑肌细胞的增殖等作用机制促进子宫切口组织修复。

关键词: 人羊膜间充质干细胞, 剖宫产, 子宫瘢痕

Abstract: Objective To exploring the effect of human amniotic mesenchymal stem cells(hAMSCs) on the repair of rat uterine scars. Methods The hAMSCs were isolated and cultured,Female SPF grade SD rats were selected for full-thickness incision of uterine wall and then implanted with hAMSCs. On the 30th day after operation, the uterine incision was examined histologically.ImageJ image analysis software was used to analyze and to compare the thickness of uterine myometrium and the percentage of fibrotic area in each group.Immunohistochemical method was used for detecting the percentage of positive areas of α-SMA, TGF-β1, and Ki-67. Results Compared with the PBS group, the hAMSCs group showed significant thickening of the uterine muscle layer and fibrotic area was decreased, The positive expression of α-SMA, and Ki-67 significantly increased(P＜0.05), while the expression of TGF-β1 was significantly reduced(P＜0.05). Conclusions The hAMSCs may promote the repair of uterine incision scars by reducing the formation of scar fibrosis and promoting the proliferation of smooth muscle cells in uterine scars.

Key words: human amniotic mesenchymal stem cell, cesarean section, uterine scar

中图分类号:

R719.8

宋佳, 赵峰, 张婷, 徐静, 孙静莉, 陈震宇. 人羊膜间充质干细胞对大鼠子宫瘢痕的修复作用[J]. 基础医学与临床, 2024, 44(7): 1002-1007.

SONG Jia, ZHAO Feng, ZHANG Ting, XU Jing, SUN Jingli, CHEN Zhenyu. Repair effect of human amniotic mesenchymal stem cells on uterine scars in rats[J]. Basic & Clinical Medicine, 2024, 44(7): 1002-1007.

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人羊膜间充质干细胞对大鼠子宫瘢痕的修复作用

Repair effect of human amniotic mesenchymal stem cells on uterine scars in rats

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