艾司氯胺酮减轻大鼠骨骼肌缺血再灌注损伤

doi:10.16352/j.issn.1001-6325.2023.12.1822

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (12): 1822-1826.doi: 10.16352/j.issn.1001-6325.2023.12.1822

艾司氯胺酮减轻大鼠骨骼肌缺血再灌注损伤

袁培根^*, 陈顺利, 单鸳露, 薛彬彬, 叶玉柱, 林丽娜

温州医科大学附属第一医院麻醉科,浙江温州 325000

收稿日期:2023-07-31 修回日期:2023-10-25 出版日期:2023-12-05 发布日期:2023-11-29
通讯作者: ^* zs202121@163.com
基金资助:
温州市科技计划项目(Y2020777)

Esketamine reduces ischemia-reperfusion injury of skeletal muscle of rats

YUAN Peigen^*, CHEN Shunli, SHAN Yuanlu, XUE Binbin, YE Yuzhu, LIN Lina

Department of Anesthesiology, the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325000,China

Received:2023-07-31 Revised:2023-10-25 Online:2023-12-05 Published:2023-11-29
Contact: ^* zs202121@163.com

摘要/Abstract

摘要： 目的观察低剂量艾司氯胺酮(ESK)预处理对于肢体缺血再灌注损伤(LI/R)的作用。方法对大鼠进行如下分组:sham组(即假手术组)、I/R组(即缺血再灌注组,对单侧肢体先实施3 h缺血处理,再实施2 h灌注)、ESK组(即ESK预处理组,经腹腔注入ESK,用量是5 mg/kg)。切取部分骨骼肌用于湿干重比检测;经由比色法测定血清内肌酸激酶(CK)、乳酸脱氢酶(LDH)的水平;再对部分骨骼肌行冻存处理(-80 ℃),用于测定丙二醛(MDA)和超氧化物歧化酶(SOD)及Western blot检测胞核核因子E2相关因子2(Nrf2)、胞质血红素加氧酶(HO-1)蛋白的表达;通过免疫组化染色(IHC),对胞核Nrf2和胞质HO-1蛋白进行检测。结果相较sham组,I/R组湿干比、CK、LDH、MDA、Nrf2和HO-1均上调,SOD下调(P＜0.05);相较I/R组,ESK组湿干比、CK 、LDH与MDA皆明显降低;而SOD、Nrf2和HO-1明显升高(P＜0.05)。结论艾司氯胺酮减轻骨骼肌缺血再灌注损伤,可能是通过提高细胞核内Nrf2水平,增强HO-1蛋白的表达来提升抗氧化的能力。

关键词: 艾司氯胺酮, 缺血/再灌注损伤, 氧化应激, 核因子E2相关因子2(Nrf2)

Abstract: Objective To investigate the role of low dose esketamine in pretreated limb ischemia-reperfusion injury. Methods The rats were divided into sham-operated group (Sham group), ischemia-reperfusion group (I/R group, 3 h of ischemia and 2 h of reperfusion), esketamine group (ESK group, ip, 5 mg/kg). The plasma concentrations of creatine kinase (CK) and lactate dehydrogenase (LDH) were measured. The wet/dry weight ratio of skeletal muscle was immediately detected. The gastrocnemius muscle was harvested and the level of malondialdehyde (MDA) and superoxide dismutase (SOD) was detected by colorimetric assay. The expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were detected by Western blot and immunohistochemical staining. Results Compared with Sham group, the index of wet/dry weight ratio, MDA, CK, LDH, Nrf2 and HO-1 were all increased, but SOD was decreased in I/R group(P＜0.05). Compared with I/R group, the index of wet/dry weight ratio, MDA, CK and LDH were significantly lower, but SOD and Nrf2 and HO-1 were significantly higher in ESK group (P＜0.05). Conclusions ESK may increase Nrf2 in the nucleus, thereby increase the HO-1 protein as an antioxidant agent.

Key words: esketamine, ischemia-reperfusion injury, oxidative stress, Nrf2

中图分类号:

R364

袁培根, 陈顺利, 单鸳露, 薛彬彬, 叶玉柱, 林丽娜. 艾司氯胺酮减轻大鼠骨骼肌缺血再灌注损伤[J]. 基础医学与临床, 2023, 43(12): 1822-1826.

YUAN Peigen, CHEN Shunli, SHAN Yuanlu, XUE Binbin, YE Yuzhu, LIN Lina. Esketamine reduces ischemia-reperfusion injury of skeletal muscle of rats[J]. Basic & Clinical Medicine, 2023, 43(12): 1822-1826.

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艾司氯胺酮减轻大鼠骨骼肌缺血再灌注损伤

Esketamine reduces ischemia-reperfusion injury of skeletal muscle of rats

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