基础医学与临床 ›› 2022, Vol. 42 ›› Issue (9): 1333-1338.doi: 10.16352/j.issn.1001-6325.2022.09.1333

• 研究论文 • 上一篇    下一篇

HMGB1减弱丹参酮ⅡA对大鼠心肌缺血/再灌注损伤的缓解作用

钟金鹏1, 杨莉2, 王辉波1, 陈红健1, 李金伟1, 文明洪1, 吕云波1*   

  1. 1. 三峡大学人民医院 宜昌市第一人民医院 心内科, 湖北 宜昌 443000;
    2. 三峡大学人民医院 宜昌市第一人民医院 神经内科, 湖北 宜昌 443000
  • 收稿日期:2021-10-11 修回日期:2021-12-31 出版日期:2022-09-05 发布日期:2022-09-02
  • 通讯作者: 62435582@qq.com
  • 基金资助:
    国家自然科学基金(82100270);湖北省卫生健康委员会联合基金(WJ2019H511)

HMGB1 attenuates the mitigative effect to myocardial ischemia-reperfusion injury by tanshinone ⅡA in rats

ZHONG Jin-peng1, YANG Li2, WANG Hui-bo1,CHEN Hong-jian1, LI Jin-wei1, WEN Ming-hong1, LYU Yun-bo1*   

  1. 1. Department of Cardiology, the People's Hospital of China Three Gorges University, Yichang 443000, China;
    2. Department of Neurology, the People's Hospital of China Three Gorges University, Yichang 443000, China
  • Received:2021-10-11 Revised:2021-12-31 Online:2022-09-05 Published:2022-09-02

摘要: 目的 研究外源性高迁移率族蛋白B1(HMGB1)对丹参酮ⅡA(TSA)缓解大鼠心肌缺血/再灌注(I/R)损伤的影响。方法 将大鼠分为假手术(sham)组、I/R组(结扎前降支冠脉)、TSA组(I/R+静脉注射TSA 10 mg/kg)、TSA+HMGB1组(I/R+静脉注射TSA 10 mg/kg+腹腔注射重组HMGB1 100 μg/kg)。再灌注24 h后,用ELISA测定肌酸激酶同工酶(CK-MB)、TCC测心梗面积,RT-qPCR及Western blot测定炎性指标表达。结果 I/R组与sham组比较,CK-MB升高(P<0.01),心肌梗死面积增大,白介素-6(IL-6)、白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的转录水平增加(均P<0.01),HMGB1、NOD样受体蛋白3(NLRP3)和胱冬肽酶-1(caspase-1)表达增加(均P<0.05);TSA组与I/R组比较,CK-MB下降(P<0.01),心梗面积缩小(P<0.01),IL-6、IL-1β和TNF-α的转录水平下降(均P<0.05),HMGB1、NLRP3、caspase-1和核因子-κB(NF-κB)表达减少(均P<0.01);而TSA+HMGB1组与TSA组比较,CK-MB升高(P<0.01),心肌梗死面积增大(P<0.01),IL-6、IL-1β和TNF-α转录水平增加(均P<0.01),HMGB1、NLRP3、caspase-1和NF-κB表达增加(均P<0.05)。结论 外源性HMGB1通过上调炎性因子表达减弱TSA缓解大鼠的心肌I/R损伤。

关键词: 高迁移率族蛋白B1, 丹参酮ⅡA, 心肌缺血/再灌注损伤, 炎性因子

Abstract: Objective To study the influence of exogenous high-mobility group box 1(HMGB1) on the mitigative effect of tanshinone ⅡA (TSA) to myocardial ischemia-reperfusion (I/R) injury. Methods Rats were divided into sham, I/R (ligation of left anterior descending coronary artery), TSA (I/R + TSA intravenous injection) and TSA+HMGB1 (I/R+TSA intravenous injection + recombination HMGB1 intraperitoneal injection) groups. Serum CK-MB was measured by ELISA. Myocardial infarct area was measured by TCC. The expression of inflammatory indicators were measured by RT-qPCR and Western blot. Results Compared with sham group, CK-MB, myocardial infarct area, transcription level of IL-6, IL-1β and TNF-α, expression of HMGB1, NLRP3 and caspase-1 all increased in I/R group(P<0.05). Compared with I/R group, CK-MB, myocardial infarct area, transcription levels of IL-6, IL-1β and TNF-α, expressions of HMGB1, NLRP3, caspase-1 and NF-κB decreased in TSA group (P<0.05). However, compared with TSA group, CK-MB, myocardial infarct area, transcription levels of IL-6, IL-1β and TNF-α, expressions of HMGB1, NLRP3, caspase-1 and NF-κB increased in TSA+HMGB1 group(P<0.05). Conclusions Exogenous HMGB1 attenuates the mitigative effect of tanshinone ⅡA to rat myocardial I/R injury through up-regulation the expressions of inflammatory factors.

Key words: high-mobility group box 1, tanshinone ⅡA, myocardial ischemia-reperfusion injury, inflammatory factor

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