甲泼尼龙琥珀酸钠减轻下肢高压喷射伤模型大鼠的肢体损害

doi:10.16352/j.issn.1001-6325.2023.11.1680

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (11): 1680-1684.doi: 10.16352/j.issn.1001-6325.2023.11.1680

甲泼尼龙琥珀酸钠减轻下肢高压喷射伤模型大鼠的肢体损害

穆钛飞, 史业弘, 王成^*, 陈世玖

遵义医科大学第五附属(珠海)医院整形手外科,广东珠海 519100

收稿日期:2022-09-30 修回日期:2023-08-12 出版日期:2023-11-05 发布日期:2023-10-30
通讯作者: ^*906090860@qq.com
基金资助:
广东省医学科学技术研究基金(A2019322)

Methylprednisolone alleviates limb damage in rat models with high-pressure injection injuries of lower limbs

MU Taifei, SHI Yehong, WANG Cheng^*, CHEN Shijiu

Department of Plastic and Hand Surgery,the Fifth Affiliated(Zhuhai) Hospital of Zunyi Medical University,Zhuhai 519100,China

Received:2022-09-30 Revised:2023-08-12 Online:2023-11-05 Published:2023-10-30
Contact: ^*906090860@qq.com

摘要/Abstract

摘要： 目的研究甲泼尼龙琥珀酸钠(MP)对大鼠下肢高压喷射伤(HPII)的干预作用。方法将大鼠随机分为对照组、模型组(高压喷射伤模型)、治疗组(尾静脉注射MP 30 mg/kg)。6 h后观察喷射面积、肢体循环情况、HE染色检查血管、神经、肌肉病理变化,计算肝指数,ELISA检验血清TNF-α、IL-1β、IL-6含量。多组间比较采用单因素方差分析。结果与对照组相比,模型组肢体循环障碍,血管、神经和肌肉病理明显断裂损伤,肝指数下降,而血清TNF-α、IL-1β和IL-6含量明显增加(P＜0.05),与模型组相比,治疗组上述指标改善明显(P＜0.05),差异有统计学意义。结论高压喷射伤后注射MP可以减轻大鼠肢体损害,降低肝脏负荷,改善血清炎性指标变化。

关键词: 高压喷射伤, 甲泼尼龙琥珀酸钠, 动物模型

Abstract: Objective To study the intervention effect of methylprednisolone(MP) on high pressure injection injuries(HPII) of lower limbs of rats. Methods Thirty rats were randomly divided into control group, model group (high-pressure injection injury model) and treatment group (tail vein injection of MP 30 mg/kg). After 6 hours, the jet area and limb circulation were observed, blood vessels, nerve and muscle tissues were examined by HE staining microscopy. Liver index was calculated and serum TNF-α, IL-1β and IL-6 were tested by ELISA. Comparison between multiple groups was performed by One-way Anova. Results Compared with the control group, limb circulation , blood vessels, nerves and muscles were significantly damaged.The liver index decreased while the serum content of TNF-α, IL-1β, and IL-6 all increased significantly (P＜0.05). Compared with the model group, the above indicators improved significantly in the treatment group(P＜0.05). Conclusions Injection of MP after high-pressure injection injuries can alleviate limb damage, reduce liver load and improve serum inflammatory markers of injured rats.

Key words: high pressure injection injuries, methylprednisolone, animal model

中图分类号:

R642

穆钛飞, 史业弘, 王成, 陈世玖. 甲泼尼龙琥珀酸钠减轻下肢高压喷射伤模型大鼠的肢体损害[J]. 基础医学与临床, 2023, 43(11): 1680-1684.

MU Taifei, SHI Yehong, WANG Cheng, CHEN Shijiu. Methylprednisolone alleviates limb damage in rat models with high-pressure injection injuries of lower limbs[J]. Basic & Clinical Medicine, 2023, 43(11): 1680-1684.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://journal11.magtechjournal.com/Jwk_jcyxylc/CN/10.16352/j.issn.1001-6325.2023.11.1680

http://journal11.magtechjournal.com/Jwk_jcyxylc/CN/Y2023/V43/I11/1680

参考文献

[1]Feldman G, Rozen N, Eliyahu AC,et al. High-pressure injection injuries of the fingers: long-term follow-up in patients after extensive debridement[J]. Hand Surg Rehabil. 2019,38:312-316.
[2]孙衍峰,窦义臣,余欣.等.手部高压注射伤的诊治策略[J].中华显微外科杂志,2019,42:399-401.
[3]Barger K,Jenkins R,Weng B,et al.A unique case report of a high-pressure paint injection injury of the hand with associated carbapenemase resistant pseudomonas aeruginosa infection[J].J Surg Case Rep,2020,2020.1-4.doi: 10.1093/jscr/rjaa154.
[4]毛文豪. 高压注射伤大鼠模型的建立及评价[D].遵义:遵义医科大学,2020:3-4.
[5]李磊,聂兰军.手部高压热塑料液体注射性损伤一例[J].中华烧伤杂志,2017,33:460-461.
[6]龙忠恒,谢卫国.湿水泥皮肤烧伤[J].中华烧伤杂志,2018,34:190-192.
[7]Eells AC,McRae M,Buntic RF,et al.High-pressure injection injury:a case report and systematic review[J].Case Reports Plast Surg Hand Surg,2019,6:153-158.
[8]Siegelman JN,Warsofsky I,Loukas BN,et al.Man with oropharyngeal trauma.Oropharyngeal high-pressure injec-tion injury[J].Ann Emerg Med,2012,59:350-368.
[9]Ryan AT,Johnstone BR.High-pressure injection injury:benign appearance belies potentially devastating consequences[J]. Med J Aust,2017,206:477.doi:10.5694/mja16.00664.
[10]徐承新,陈黎明,陈亮,等.高压水泥浆致腹股沟与大腿内侧皮肤软组织损伤的救治与修复[J].中国美容医学,2019,28:8-10.
[11]Cannon TA.High-pressure injection injuries of the hand[J].Orthop Clin North Am,2016,47:617-624.
[12]Kelpin JP,Fahrenkopf MP,Van Pelt AE.Treatment of an uncommon high-pressure orbital injection injury[J].J Craniofac Surg,2018,29:1829-1831.
[13]李大为,余迎浩,姜德欣,等.手部高压油漆注射伤的手术疗效分析[J].中华手外科杂志,2020,36:227-228.
[14]Feldman G,Hitti S,Rozen N,et al.Molten metal high pressure injection injury of the hand[J].Hand Surg Rehabil,2020,39:328-331.
[15]Strehl C,Spies CM,Buttgereit F. Pharmacodynamics of glucocorticoids.[J]. Clin Exp Rheumatol,2011,29,13-18.
[16]曾灵,顾玮,严军,等.人糖皮质激素受体调控靶基因的生物信息学分析[J].基础医学与临床,2008,11:1146-1150.

甲泼尼龙琥珀酸钠减轻下肢高压喷射伤模型大鼠的肢体损害

Methylprednisolone alleviates limb damage in rat models with high-pressure injection injuries of lower limbs

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 12

编辑推荐

Metrics

本文评价

[1]	刘淑芬王琦马亚曾学军. 长期单纯高尿酸血症大鼠主动脉病理变化[J]. 基础医学与临床, 2018, 38(5): 692-695.
[2]	陈云飞马百涛薛春玲韩钦魏俊吉赵春华常健博. 人脂肪间充质干细胞来源的外排体促进大鼠创伤性脑损伤后神经功能恢复[J]. 基础医学与临床, 2017, 37(6): 802-807.
[3]	肖鲁瑶张杰徐志刚郑帅梅少帅. 雨蛙素及脂多糖诱导小鼠急性胰腺炎效果分析[J]. 基础医学与临床, 2017, 37(10): 1384-1388.
[4]	张颖高金环韩松李俊发. 一种改良的三血管闭塞小鼠缺血性脑卒中模型的建立[J]. 基础医学与临床, 2017, 37(1): 38-42.
[5]	许婷李昕冯异. 多囊卵巢综合征转基因动物模型的研究进展[J]. 基础医学与临床, 2013, 33(1): 119-122.
[6]	吴超余上斌. 临床相关的脊髓损伤动物模型新进展[J]. 基础医学与临床, 2012, 32(8): 968-970.
[7]	陈莹常志文杨琳翟艳苓张勇林文娟. 大鼠抑郁障碍参与启动心血管组织NF-κB表达增强 [J]. 基础医学与临床, 2011, 31(6): 700-703.
[8]	刘淑芬曾学军. 高尿酸血症动物模型研究进展 [J]. 基础医学与临床, 2011, 31(3): 344-347.
[9]	高海成高海梅皱颖刚任立群. 阿魏酸钠抑制大鼠缺血坏死模型中TGF-β1表达[J]. 基础医学与临床, 2010, 30(9): 940-943.
[10]	朱亮邢福祺全松张文颖杜建新. 多囊卵巢综合征大鼠模型LHR、INSR、AR基因甲基化的变化[J]. 基础医学与临床, 2010, 30(1): 63-66.
[11]	陈华夏高金明蒋磊聂莉郭子建. 粉尘螨蛋白致敏小鼠肺部变应性炎症模型的建立[J]. 基础医学与临床, 2009, 29(9): 901-904.
[12]	刘凤君刘丽蒋智刘聪唐红. 恩替卡韦抑制乙型肝炎病毒复制型小鼠体内病毒的复制[J]. 基础医学与临床, 2009, 29(10): 1017-1020.