转化生长因子β1促进大鼠子宫内膜纤维化

doi:10.16352/j.issn.1001-6325.2023.10.1557

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (10): 1557-1561.doi: 10.16352/j.issn.1001-6325.2023.10.1557

转化生长因子β1促进大鼠子宫内膜纤维化

金国钰¹, 杨春润^2,3, 何静¹, 张舒荣^2,3, 刘欢欢¹, 李长忠^3,4*

1.山东中医药大学第一临床医学院,山东济南 250000;
2.山东省立医院妇科,山东济南 250021;
3.山东大学医学融合与实践中心,山东济南 250021;
4.北京大学深圳医院妇产科,广东深圳 518036

收稿日期:2023-02-17 修回日期:2023-05-26 出版日期:2023-10-05 发布日期:2023-10-05
通讯作者: *15168888909@163.com
基金资助:
济南市科学计划项目(202019161);山东大学荣祥再生医学基金项目(2019SDRX-21)

Transforming growth factor-beta 1 promotes endometrial fibrosis in rats

JIN Guoyu¹, YANG Chunrun^{2, 3}, HE Jing¹, ZHANG Shurong^{2, 3}, LIU Huanhuan¹, LI Changzhong^{3, 4*}

1. the First Clinical Medical College, Shandong University of Traditional Chinese Medicine,Jinan 250000;
2. Department of Gynecology, Shandong Provincial Hospital, Jinan 250021;
3. Center for Medical Integration and Practice, Shandong University, Jinan 250021;
4. Department of Obstetrics and Gynecology, Peking University Shenzhen Hospital,Shenzhen 518036, China

Received:2023-02-17 Revised:2023-05-26 Online:2023-10-05 Published:2023-10-05
Contact: *15168888909@163.com

摘要/Abstract

摘要： 目的探讨大鼠子宫内膜纤维化中转化生长因子β1(TGF-β1)的表达及相关机制。方法将SD 雌性大鼠随机分成假手术组(n=12)和模型组(n=12)。术后7、14及28 d收集双侧子宫组织,HE染色观察子宫内膜形态,动态观察子宫内膜病理改变和子宫内膜腺体数目;Masson 染色检测子宫内膜纤维化程度;免疫组织化学法检测TGF-β1及α-SMA 蛋白表达。结果相比假手术组,模型组大鼠子宫结构破坏;术后7、14和28 d,子宫内膜TGF-β1蛋白表达显著升高(P＜0.05);术后14和28 d,子宫内膜腺体数目明显减少、子宫内膜纤维化面积比例明显增加,α-SMA蛋白表达上调(P＜0.05)。结论 TGF-β1可能通过激活肌成纤维细胞及相关信号通路进一步促进子宫内膜纤维化。

关键词: 宫腔黏连, 纤维化, 大鼠, 转化生长因子β1, α-平滑肌肌动蛋白

Abstract: Objective To investigate the expression of transforming growth factor-beta 1 in endometrial fibrosis in rats and related mechanisms. Methods Sprague-Dawley female rats were randomly divided into sham operation group (n=12) and model group (n=12). Uterine tissues were collected on days 7, 14 and 18 after surgery. HE staining and Masson staining were measured the number of endometrial glands and degree of endometrial fibrosis. The protein expression of TGF-β1 and α-SMA were measured by immunohistochemistry. Results Compared with the sham operation group, the uterine structure of the rats in the model group was significantly disrupted; there was a significant increase in TGF-β1 expression in the model group at 7 days,14 days, and 28 days of modeling; the number of endometrial glands decreased, the fibrotic area of endometrium increased and the expression of α-SMA was significantly higher at 14 days and 28 days of modeling. Conclusions TGF-β1 may promote endometrial fibrosis by activating myofibroblasts.

Key words: intrauterine adhesions, fibrosis, rat, TGF-β1, α-SMA

中图分类号:

R217.9

金国钰, 杨春润, 何静, 张舒荣, 刘欢欢, 李长忠. 转化生长因子β1促进大鼠子宫内膜纤维化[J]. 基础医学与临床, 2023, 43(10): 1557-1561.

JIN Guoyu, YANG Chunrun, HE Jing, ZHANG Shurong, LIU Huanhuan, LI Changzhong. Transforming growth factor-beta 1 promotes endometrial fibrosis in rats[J]. Basic & Clinical Medicine, 2023, 43(10): 1557-1561.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://journal11.magtechjournal.com/Jwk_jcyxylc/CN/10.16352/j.issn.1001-6325.2023.10.1557

http://journal11.magtechjournal.com/Jwk_jcyxylc/CN/Y2023/V43/I10/1557

参考文献

[1]刘静乔,孟亚丽,徐淑稳,等.丹参酮ⅡA对宫腔黏连模型大鼠子宫内膜容受性的改善作用及其机制[J].吉林大学学报(医学版),2021,4:118-124.
[2]笪琳萃,郑备红,孙艳,等.化学和热传导双重损伤构建大鼠宫腔黏连模型的方法及其评价[J].吉林大学学报(医学版),2023,49:215-221.
[3]Lee WL, Liu CH, Cheng M, et al. Focus on the primary prevention of intrauterine adhesions: current concept and vision[J]. Int J Mol Sci, 2021, 22:5175. doi: 10.3390/ijms22105175.
[4]Hou TY, Wu SB, Kau HC, et al. JNK and p38 inhibitors prevent transforming growth factor-β1-induced myofibro-blast transdifferentiation in human graves′ orbital gibroblasts[J]. Int J Mol Sci, 2021, 22:2952.doi: 10.3390/ijms22062952.
[5]Feng Q, Gao B, Zhao X, et al. Establishment of an animal model of intrauterine adhesions after surgical abortion and curettage in pregnant rats[J]. Ann Transl Med, 2020.doi:10.21037/ATM.2020.01.134
[6]姜艳玲,丁丽,李发余,等.榆栀止血颗粒减轻宫腔粘连大鼠子宫内膜纤维化[J].基础医学与临床,2022,42:1220-1224.
[7]Evangelia P, Gonzalo SD, Maria GP, et al. TGF-β-induced endothelial-mesenchymal transition in fibrotic diseases[J]. Int J Mol Sci, 2017,18:2157. doi: 10.3390/ijms18102157
[8]Marconi GD, Fonticoli L, Rajan TS, et al. Epithelial-mesenchymal transition (EMT): the type-2 EMT in wound healing, tissue regeneration and organ fibrosis[J].Cells, 2021,10:1587.doi:10.3390/cells10071587.
[9]Elson EL, Hong Q, Fee JA, et al. A model for positive feedback control of the transformation of fibroblasts to myofibroblasts[J]. Prog Biophys Mol Biol, 2019, 144:30-40.
[10]Fukuto A, Kim S, Kang J, et al. Metal oxide engineered nanomaterials modulate rabbit corneal fibroblast to myofibroblast transformation[J]. Transl Vis Sci Technol, 2021,10:23.doi:10.1167/tvst.10.12.23
[11]郭意欣,关婷.机械损伤联合不同感染方法建立大鼠宫腔黏连模型的对比研究[J].现代妇产科进展,2018,27:693-695.

[1]	陈新军, 王卿语, 陈乐, 古春昱, 武卓. 紫檀茋减轻百草枯诱导的模型大鼠肺纤维化[J]. 基础医学与临床, 2023, 43(9): 1383-1389.
[2]	方路, 刘睿奇, 梁鹏, 岑瑛. 外泌体miRNAs在器官纤维化中作用的研究进展[J]. 基础医学与临床, 2023, 43(9): 1472-1476.
[3]	杨雅倩, 潘艳芳, 屈梦扬, 方艳, 应小平, 张玫倩, 魏静. 外泌体在肝癌前病变发生发展中作用的研究进展[J]. 基础医学与临床, 2023, 43(9): 1453-1456.
[4]	周王继, 孙艳艳, 程重生, 赵阳, 韩江娜, 戴金荣, 孟淑珍, 徐凯峰, 田欣伦. 中国囊性纤维化患者单中心焦虑抑郁状况调查[J]. 基础医学与临床, 2023, 43(4): 647-650.
[5]	王琴, 张晓艳, 刘雪婷, 李炳蔚, 李宏伟, 杨义力, 韩建群. 全杜仲胶囊对自发性高血压大鼠肠壁微血流灌注的影响[J]. 基础医学与临床, 2023, 43(3): 386-392.
[6]	徐倩, 段华, 甘露, 刘麒薇. RhoGTP酶活化蛋白在上皮细胞间质转化中作用的研究进展[J]. 基础医学与临床, 2023, 43(2): 301-305.
[7]	李美崎, 邓俊, 王宋平. 苦参碱减轻百草枯诱导的模型小鼠肺纤维化[J]. 基础医学与临床, 2022, 42(9): 1385-1390.
[8]	姜艳玲, 丁丽, 李发余, 解世雷, 张一琼. 榆栀止血颗粒减轻宫腔粘连大鼠子宫内膜纤维化[J]. 基础医学与临床, 2022, 42(8): 1220-1224.
[9]	田平平, 宁洁, 邹琴, 卢雨微, 郭兵, 石明隽. 5-氮杂-2’-脱氧胞苷对高糖培养的NRK-52E细胞中分泌型卷曲相关蛋白1表达的影响[J]. 基础医学与临床, 2022, 42(8): 1194-1199.
[10]	王立铨, 黄久佐, 俞楠泽, 马旭达, 李天浩, 龙笑. hsa-Let-7a-5p靶向结合TGF-βR1抑制局限性硬皮病成纤维细胞纤维化[J]. 基础医学与临床, 2022, 42(12): 1841-1849.
[11]	祁俊清, 曹梦菲, 赵倩茹, 尹俊. 线粒体SIRT3在器官纤维化中作用的研究进展[J]. 基础医学与临床, 2022, 42(11): 1799-1803.
[12]	易达松, 袁善斌, 曾俊义, 赵健清, 丁露, 文通, 聂俊刚. 自发性高血压大鼠年龄相关性左心室肥厚的动态变化[J]. 基础医学与临床, 2022, 42(10): 1481-1486.
[13]	刘胜来, 田大伟, 吴长利. 梗阻性膀胱逼尿肌功能失调分子机制的研究进展[J]. 基础医学与临床, 2022, 42(10): 1606-1610.
[14]	李建国, 王颖, 李玥天, 燕子, 赵欣, 张宇. MK-801对皮质酮诱导抑郁样行为大鼠海马mBDNF和proBDNF表达的影响[J]. 基础医学与临床, 2021, 41(8): 1097-1102.
[15]	贾春松, 赵彦坡, 尚振华, 邢添瑛, 欧彤文. RNA干扰降低膀胱TGF-β1表达能够改善脊髓损伤大鼠的膀胱功能和纤维化[J]. 基础医学与临床, 2021, 41(7): 1007-1012.

转化生长因子β1促进大鼠子宫内膜纤维化

Transforming growth factor-beta 1 promotes endometrial fibrosis in rats

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价