CRISPR/Cas9技术在CAR-T细胞治疗肿瘤中作用的研究进展

doi:10.16352/j.issn.1001-6325.2023.08.1313

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (8): 1313-1316.doi: 10.16352/j.issn.1001-6325.2023.08.1313

CRISPR/Cas9技术在CAR-T细胞治疗肿瘤中作用的研究进展

伍琦, 王绍波^*

昆明理工大学附属医院云南省第一人民医院 PET/CT中心,云南昆明 650032

收稿日期:2022-03-10 修回日期:2023-01-06 出版日期:2023-08-05 发布日期:2023-07-26
通讯作者: ^*15812082912@126.com
基金资助:
国家自然科学基金(81760306);云南省卫生高层次人才(医学学科带头人)(D-2018011);云南省“万人计划”青年拔尖人才项目(YNWR-QNBJ-2018-243)

Progress in CRISPR/Cas9 for CAR-T cell therapy of tumors

WU Qi, WANG Shaobo^*

PET/CT Center, the Affiliated Hospital of Kunming University of Science and Technology, the First People's Hospital of Yunnan Province, Kunming 650032, China

Received:2022-03-10 Revised:2023-01-06 Online:2023-08-05 Published:2023-07-26
Contact: ^*15812082912@126.com

摘要/Abstract

摘要： CRISPR/Cas9基因编辑技术能有效解决嵌合抗原受体T细胞(CAR-T)疗法中T细胞来源不足的问题,并通过敲除T细胞活性抑制的相关基因,增强其肿瘤杀伤作用。此外,该技术还可用于构建具有双重靶向作用的T细胞,阻止肿瘤免疫逃逸,减少治疗后复发、耐药等情况的发生。相较于其他基因工程技术的复杂过程,CRISPR/Cas9具有便捷高效及多重基因组编辑的优点,对完善CAR-T治疗策略具有重要意义。

关键词: 嵌合抗原受体T细胞, CRISPR/Cas9, 免疫治疗

Abstract: CRISPR/Cas9 gene editing technology can effectively solve the problem of insufficient source of T cells in chimerical antigen receptor-T (CAR-T) therapy and enhance their anti-tumor abilities by knocking out the genes related to the suppression of T cells activity. The technology can also be used to construct T cells with dual-targeting effect to stop tumor immune escape and inhibit recurrence and drug resistance development during chemotherapy. Compared with the other genetic engineering technologies that have complex processes, multiple genome editing by CRISPR/Cas9 is a technology of efficient and convenient to operate.So it is believed to be valuable and potential target molecules for CAR-T therapy.

Key words: CAR-T cells, CRISPR/Cas9, immunotherapy

中图分类号:

伍琦, 王绍波. CRISPR/Cas9技术在CAR-T细胞治疗肿瘤中作用的研究进展[J]. 基础医学与临床, 2023, 43(8): 1313-1316.

WU Qi, WANG Shaobo. Progress in CRISPR/Cas9 for CAR-T cell therapy of tumors[J]. Basic & Clinical Medicine, 2023, 43(8): 1313-1316.

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CRISPR/Cas9技术在CAR-T细胞治疗肿瘤中作用的研究进展

Progress in CRISPR/Cas9 for CAR-T cell therapy of tumors

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