中国现代神经疾病杂志 ›› 2020, Vol. 20 ›› Issue (9): 788-793. doi: 10.3969/j.issn.1672-6731.2020.09.006

• 神经免疫性疾病 • 上一篇    下一篇

2 CD4-CD8-双阴性T淋巴细胞在视神经脊髓炎谱系疾病与多发性硬化中的表达差异

沙晶, 杨丽娟, 玛依努尔·买买提, 李红燕   

  1. 830000 乌鲁木齐, 新疆维吾尔自治区人民医院神经内科
  • 收稿日期:2020-09-15 出版日期:2020-09-25 发布日期:2020-09-30
  • 通讯作者: 李红燕,Email:lhyxxy@vip.163.com
  • 基金资助:

    新疆维吾尔自治区自然科学基金资助项目(项目编号:2019D01C150)

The differential expression of CD4- CD8- double negative T lymphocytes in neuromyelitis optica spectrum disorders and multiple sclerosis

SHA Jing, YANG Li-juan, Mayinuer Maimaiti, LI Hong-yan   

  1. Department of Neurology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830000, Xinjiang, China
  • Received:2020-09-15 Online:2020-09-25 Published:2020-09-30
  • Supported by:

    This study was supported by Natural Science Foundation of Xinjiang Uygur Autonomous Region (No. 2019D01C150).

摘要:

目的 对比分析视神经脊髓炎谱系疾病(NMOSDs)与多发性硬化患者急性期外周血CD4-CD8-双阴性T细胞(DNT)表达差异。方法 纳入2017年1月至2019年12月诊断明确的NMOSDs(53例)和多发性硬化(20例)患者,以及性别、年龄相匹配的正常对照者(27例),流式细胞术测定外周血DNT细胞数目。结果 3组受试者外周血DNT细胞数目差异具有统计学意义(F=4.362,P=0.015),其中NMOSDs组DNT细胞数目分别高于正常对照组(t=2.415,P=0.023)和多发性硬化组(t=2.415,P=0.018),而多发性硬化组与正常对照组DNT细胞数目差异无统计学意义(t=0.149,P=0.881)。。结论 NMOSDs急性期患者外周血DNT细胞数目高于多发性硬化患者和正常对照者,推测DNT细胞可能参与NMOSDs的发病机制。

关键词: 视神经脊髓炎, 多发性硬化, T淋巴细胞亚群, 流式细胞术

Abstract:

Objective To compare the levels of CD4-CD8- double negative T lymphocytes(DNT) in the peripheral blood of patients with neuromyelitis optica spectrum disorders (NMOSDs) and multiple sclerosis (MS) in the acute phase. Methods From January 2017 to December 2019, a total of 53 patients with NMOSDs, 20 patients with MS and 27 normal controls were included. Peripheral blood DNT cells of the patients in 3 groups were measured by flow cytometry. Results The differences of DNT cells in the peripheral blood in 3 groups were statistically significant (F=4.362, P=0.015). Among them, the number of DNT cells in the NMOSDs group was higher than that in the control group (t=2.415, P=0.023) and the MS group (t=2.415, P=0.018), while the number of DNT cells in the MS group and the control group was not statistically different (t=0.149, P=0.881). Conclusions The number of DNT cells in the peripheral blood of patients with NMOSDs in the acute phase is higher than that in patients with MS and normal controls. It is speculated that DNT cells may be involved in the pathogenesis of NMOSDs.

Key words: Neuromyelitis optica, Multiple sclerosis, T-lymphocyte subsets, Flow cytometry