摘要：
目的 探讨神经生长因子对糖皮质激素诱导的大鼠海马神经元凋亡的保护作用。 方法 体外分离原代培养18 只新生Wister 大鼠海马神经元，噻唑蓝法测定地塞米松诱导海马神经元凋亡的最低敏感剂量，观察不同质量浓度神经生长因子对地塞米松（0.10 × 106 mol/L）诱导海马神经元凋亡的保护作用。 结果 与阴性对照组相比，地塞米松Ⅰ组（10 × 106 mol/L）、Ⅱ组（1 × 106 mol/L）和Ⅲ组（0.10 × 106 mol/L）大鼠海马神经元活性均降低（P = 0.000，0.000，0.000）。予不同质量浓度神经生长因子后，神经生长因子0.18 ng/ml 组大鼠海马神经元活性低于阴性对照组（P = 0.000）和阳性对照组（P = 0.010），神经生长因子18 ng/ml 组大鼠海马神经元活性高于阳性对照组（P = 0.000）和神经生长因子0.18 ng/ml 组（P = 0.000）。 结论 糖皮质激素可以诱导体外培养的大鼠海马神经元凋亡，地塞米松0.10 × 106 mol/L是诱导海马神经元凋亡的最低敏感剂量，神经生长因子可以拮抗地塞米松诱导的大鼠海马神经元凋亡。
关键词:
神经生长因子,
糖皮质激素类,
海马,
细胞凋亡,
细胞, 培养的,
疾病模型, 动物
Abstract:
Objective To observe the protective effect of nerve growth factor (NGF) on apoptosis of primary cultured rat hippocampal neurons which were induced by glucocorticoids. Methods The neurons isolated from the hippocampus of 18 neonatal Wister rats were cultured in vitro. Methyl thiazolyl tetrazolium (MTT) analysis was used to detect the lowest concentration of dexamethasone-induced hippocampal neuronal apoptosis, so as to explore the protective effect of different concentrations of NGF on 0.10 × 106 mol/L dexamethasone-induced hippocampal neuronal apoptosis. Results Compared with negative control group, the activity of rat hippocampal neurons was reduced significantly in dexamethasone Ⅰ (10 × 106 mol/L), Ⅱ (1 × 106 mol/L) and Ⅲ (0.10 × 106 mol/L) groups (P = 0.000, 0.000, 0.000). After different concentrations of NGF were given, the activity of hippocampal neurons in NGF 0.18 ng/ml group was significantly lower than negative control group (P = 0.000) and positive control group (P = 0.010), while the activity of hippocampal neurons in NGF 18 ng/ml group was significantly higher than positive control group (P = 0.000) and NGF 0.18 ng/ml group (P = 0.000). Conclusions Glucocorticoids can induce the apoptosis of in vitro cultured rat hippocampal neurons, and 0.10 × 106 mol/L dexamethasone is the lowest sensitive dose. NGF plays a role of blocking dexamethasone-induced apoptosis.
Key words:
Nerve growth factor,
Glucocorticoids,
Hippocampus,
Apoptosis,
Cells, cultured,
Disease models, animal
夏颖华, 孔晓冬, 雷平, 张释双, 张明义, 赵子龙, 葛歆瞳. 神经生长因子对糖皮质激素诱导大鼠海马神经元凋亡的保护作用[J]. 中国现代神经疾病杂志, 2017, 17(03): 209-213.
XIA Ying-hua, KONG Xiao-dong, LEI Ping, ZHANG Shi-shuang, ZHANG Ming-yi, ZHAO Zi-long, GE Xin-tong. Protective effect of nerve growth factor on glucocorticoid-induced apoptosis of primary cultured rat hippocampal neurons[J]. Chinese Journal of Contemporary Neurology and Neurosurgery, 2017, 17(03): 209-213.