Table of Content

05 March 2017, Volume 37 Issue 3

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Clinical trial protocol for bone marrow mesenchymal stem cell transplantation to treat acute myocardial infarction

2017, 37(3):  291-295. 

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Clinical trial protocol for bone marrow mesenchymal stem cell transplantation to treat chronic graft versus host disease

2017, 37(3):  296-299. 

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Expression of inflammatory factors are increased by sorbitol in lumbar spinal stenosis of diabetic patients

2017, 37(3):  300-306. 

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Objective Across sectional study was undertaken to investigate the related mechanism of ligamentum flavum (LF) hypertrophy in diabetic patients with lumbar spinal canal stenosis (LSCS).Methods Twenty-four diabetes mellitus patients [DM (+)]and twenty normoglycemic patients [DM (-)]with LSCS were enrolled in this study. Sorbitol in LF was analyzed using D-Sorbitol/Xylitol test kit. The thickness of LF was measured by CT. The structure of LF was observed after HE and Masson’s trichrome staining. The cell cycle and proliferation of fibroblastic cell NIH3T3 line cultured in high glucose was analyzed. Sorbitol of NIH3T3 was detected under different backgrounds in vitro, normal glucose, high glucose and high glucose burdened with aldose reductase inhibitor(ARI), Epalrestat. The expressions of inflammatory factors were detected by qPCR and Western-blot under above different backgrounds. Results LF of diabetic patients exhibited significantly higher levels of sorbitol and pro-inflammatory cytokines, TGF-β and of CD68-positive staining than that of the normoglycemic subjects( P<0.01). The diabetic LF was significantly thicker than that of the controls, and showed evidence of degeneration. The high glucose-cultured fibroblasts exhibited significantly higher levels of sorbitol, pro-inflammatory factors, and TGF-β compared to the low glucose-cultured cells, and these levels were dose-dependently reduced by treatment with the aldose reductase inhibitor( P<0.05). Conclusions Sorbitol level of the LF is significantly increased in the DM patients with LSCS. Increased sorbitol recruites inflammatory factors and fibrogenic-related factor TGF-β in LF of DM patients with LSCS which contributes to the LF hypertrophy.

Senescence induced by D-galactose and its biological mechanism in rat bone marrow stromal cells

2017, 37(3):  307-312. 

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Objective To establish the aging model of rat bone marrow stromal cells (BMSCs) in vitro and in vivo, in order to study the senescence biology of aging BMSCs. Methods The control cell group (in vitro): isolating, purifying and culturing BMSCs from healthy male SD rats. collecting the third generation (P3) of BMSCs for analysis. The aging model group (in vitro): the P3 BMSCs was co-cultured with D-galactose (D-Gal, 30 g/L) for 48 hours. The aging rat model group (in vivo): the rat were given 120 mg D-Gal by the way of daily neck subcutaneous injection for 42 consecutive days. The control rat group (in vivo): the rats were administrated with the same volume of saline for the same times. On the second day after the aging model was established, the BMSCs were collecting and culturing for study. 1)The proliferative potency was detected by Cell Counting Kit-8(CCK-8); the distribution of cell cycle and apoptosis by flow cytometry (FCM); 2)the ratio of aging BMSCs by the senescence-associated β-galactosidase(SA-β-Gal) staining; 3)malonaldehyde(MDA) content and total superoxide dismutase(SOD) activity by enzymatic assay; the level of reactive oxygen species (ROS) by DCFH-DA fluorescent staining counted with FCM; 4)the expression level of senescence-related signaling proteins of P16,P21,P53,CDK2 and cyclin-D by Western blotting. Results Compared with the matched control group, the BMSCs of aging model group displayed a significant decrease in proliferation; the BMSCs were held in G1 phase arrest as the proportion of the cells in G1 phase increased, while that decreased in S phase(P<0.05); and the positive ratio of SA-β-Gal stained BMSCs also significantly increased(P<0.05); BMSCs in the aging model group showed an increasing level of ROS and MDA, meanwhile a decline in total SOD activity was decreased(P<0.05);P16,P21 and P53 protein expression in aging BMSCs was obviously enhanced(P<0.05), at the same time the expression of CDK2 and cyclin-D was also decreased(P<0.05). Conclusions D-Gal can be used to build the aging model of BMSCs equally in vitro and vivo, It acts through up-regulation of expressions of aging-related proteins and inhibition of level of oxidative stress injury and chronic inflammation.

Preincubation with low dose of hydrogen peroxide enhance BMSCs anti-oxidative stress potential of mouse

2017, 37(3):  313-319. 

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Object To investigate the effects of preconditioning with low-concentration hydrogen peroxide （H2O2）on oxidative stress-induced bone marrow mesenchymal stem cells (BMSCs) apoptosis and its mechanism．Methods Mouse bone marrow mesenchymal stem cells (BMSCs) were isolated and purified by differential centrifugation, and were treated with 0，200，250，300， 500 μmol/L H2O2 after being preincubated with 50 μmol/L H2O2 or control medium. Apoptosis of these cells was measured by flow cytometry, and the expression of phosphorylated PI3K, Akt and mTOR was analyzed by Western blot; BMSCs were also primed with PI3K inhibitor LY294002 for 30 min, then preincubated with 50 μmol/L H2O2 or control medium for 12 h before treatment with 300 μmol/LH2O2. Expression of apoptosis proteins Bcl-2, Bax, caspaase-3, cleaved-caspase-3 and the key proteins of the PI3K/Akt/mTOR pathway were detected by Western blot. Results As demonstrated by flow cytometry results, H2O2 induced BMSCs apoptosis in a dose-dependent manner，and pretreatment of BMSCs with low concentration of H2O2 significantly decreased H2O2-induced apoptosis of the BMSCs. Western blot results revealed that preconditioning with low-concentration H2O2 remarkably reversed the decrease in Bcl-2, total and phosphorylated PI3K, Akt and mTOR levels, and the increase in Bax, cleaved-caspase-3 expression after high-dose H2O2 treatment. Such effects were abolished by PI3K inhibitor LY294002. Conclusions Preincubation with low-concentration H2O2 may grant resistance of BMSC to oxidative stress, and such effect may involve inhibition of pro-apoptotic proteins and activation of the PI3K/Akt/mTOR pathway.

Resveratrol inhibits high glucose-induced hypertrophy in myocardial cell line H9C2

2017, 37(3):  320-324. 

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Objective To investigate the effect of resveratrol on high glucose-induced oxidative stress and cardiomyocyte hypertrophy, together with its possible mechanism. Methods H9C2 cardiomyocytes were divided into normal glucose group, high glucose group, resveratrol group and resveratrol + DAPT group, respectively. The cell surface area was measured by rhodamine-labeled phalloidin. Reactive oxygen species generation was measured using DCFH-DA. The contents of SOD and MDA were evaluated by colorimetry. The mRNA and protein levels of Notch1, hes1, ANP and BNP were evaluated by RT-qPCR and western blot. Results Compared to normal glucose group, high glucose exposure significantly increased cell surface area（p < 0.01）and enhanced ROS and MDA generation（p < 0.01）with concomitant decreased SOD activity（p < 0.01）, as well as decreased the mRNA and protein expressions of Notch1 and hes1（p < 0.05）, and increased the expression levels of ANP and BNP（p < 0.05）. Resveratrol treatment suppressed increased cell surface area（p < 0.01）and decreased ROS and MDA generation（p < 0.01）with concomitant enhanced SOD activity（p < 0.01）, as well as increased the expression levels of Notch1 and hes1（p < 0.05）, and decreased the expression levels of ANP and BNP（p < 0.05）. Conclusions Resveratrol may inhibit high glucose induced H9C2 cardiomyocytes hypertrophy by improving oxidative stress and activating Notch1/hes1 signaling pathway．

Human embryonic stem cells inhibits the proliferation and promote apoptosis of SK-Hep human hepatoma cell line SK-Hep1

2017, 37(3):  325-329. 

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Objective To explore effects of human embryonic stem cells ( hESCs ) onhproliferation , invasion and migration abilities of SK-Hep1 humanhepatoma cells in the co-culture micro environmen of hESCs and SK - Hep1. Methods Single cultured SK-Hep1 cells were served as control group while SK-Hep1 which non-contact co-cultured with hESCs was regarded as experimental group.The hproliferation ability of SK-Hep1 was measured by MTT method; invasion and migration ability of SK-Hep1 cells were detected by Transwell chamber method ; the nucleus variation and cell apoptosis of SK-Hep1 were detected by Hoechst33258 chromosome and flow cytometry. ResultsThe proliferation ability of SK-Hep1 cells in the experimental group was inhibited obviously compared with control group (P <0.05); the number of SK - Hep1 cells which passed through the Transwell chambers were reduced significantly compared with control group in invasion and migration experiment(P<0.05); more nucleus pycnosis and deformation appeared in experimental group than that in control group .And apoptosis rate of SK- Hep1 cells in the experimental group was significantly higher than that of in the control group (P <0.05). Conclusions Human embryonic stem cells have inhibitory effect on SK-Hep1 human hepatoma cell line.

Clinical profile and visual outcomes after treatment in patients with dysthyroid optic neuropathy

2017, 37(3):  330-334. 

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Objective:To report the clinical data and visual outcomes after treatment of patients with dysthyroid optic neuropathy (DON).Methods We retrospectively examined the clinic data of the 38 patients (67 eyes) suffering from DON and analysed the effect of glucocorticoids, radiation and orbital decompression.ResultsThe study included 14 men and 24 women. 32 patients (84.2%) received multiple treatment modalities.LogMAR vision acuity changed from (0.60±0.59) before treatment to (0.18±0.09) after treatment(P<0.01) . There were 59 eyes (88.1%) in the treatment effective group, 8 eyes (11.9%) in the ineffective group.38 eyes underwent initial treatment with intravenous steroid pulse therapy and 35 eyes (92.1%) were effective.29 eyes were treated with other modalities and 24 eyes(82.8%) were effective（P<0.01）. There were 3 eyes (5.1%) suffering from fixed eyeball movement in the effective group, while 4 eyes (50%) in the ineffective group (P<0.05). The thickness of the supraocular muscle group in the effective group was（7.63±1.19）mm, （8.81±0.83）mmin the ineffective group(P<0.05).Mean defect of the visual field was（2.41±2.82） in the effective group and （11.98±7.07） in the ineffective group(P<0.05).Conclusions:Treatment with multiple modalities effectively improved visual outcomes in cases of DON.Intravenous steroid pulse therapy was suggested as the initial modality.

Effects of ZEB1 gene knockdown on proliferation,cell cycle and apoptosis of human adipose-derived mesenchymal stem cells

2017, 37(3):  335-340. 

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Objective To investigate the effects of ZEB1 (Zinc finger E-box binding homeobox 1) gene knockdown on proliferation, cell cycle and apoptosis of human adipose-derived mesenchymal stem cells(hADSCs). MethodsPrimary mesenchymal stem cells were obtained from human adipose tissue by collagenase digestion and identified by immunological phenotype and differentiation potential of osteogenic and adipogenic lineages. The siRNA was transfected into hADSCs by lipofectamine 2000.The expression of ZEB1 and key proliferation, cell cycle and apoptosis-related genes were detected by real-time PCR and Western blot at mRNA and protein level respectively. The cell proliferation capacity was assessed by MTS. The apoptosis and cell cycle of hADSCs were evaluated by flow cytometry. ResultsThe ZEB1 expression at mRNA and protein level was significantly decreased in si-ZEB1 transfection cells compared to transfection with si-NC. Inhibition of ZEB1 decreased the cell proliferation ability, and significantly inhibited the expression of cell proliferation related genes,including CCND1,MKI67, MYC and PCNA. After transfection withsi-ZEB1, the percentage of cells in G1 phase increased from 50.17% to 58.94%, and S phase and G2 phase decreased by 6.16% and 2.07% separately.Meanwhile, the apoptosis rate increased by 10.2%, the expression of apoptosis related genes TP53 and BAX was up-regulated, and the expression of anti-apoptotic genes MCL1 and BCL2was down-regulated in si-ZEB1 transfection cells compared to transfection with si-NC. ConclusionsZEB1 may play an important role in promoting hADSCs proliferation, cell cycle progression and inhibit their apoptosis.

High level insulin increases expression of angiogenensis II in the endometrium of early pregnant mice

2017, 37(3):  341-345. 

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Objective To investigate the effect of high level insulin on the vascular marker molecules Ang-Ⅱ in the endometrium of early pregnant mice. Methods The high insulin animal model was constructed by subcutaneous injection of insulin. The uterine tissues and serum of D6, D7, and D8 days of pregnancy which were collected after mating with normal male rats, and blood glucose was monitored simultaneously. In addition to the male rats with vasectomized mating to construct artificial decidualization. ELISA for the detection of serum insulin, progesterone, and estradiol changes, real time PCR to detect the Ang-Ⅱ mRNA expression in endometrial tissue. Western blot for the detection of Ang-Ⅱ protein expression in uterine tissue. Results Insulin in the high insulin group of pregnancy D6, D7, and D8 days increased significantly(P<0.001), estrogen levels in pregnancy D8 days appear significantly decreased(P<0.05) and progesterone levels in pregnancy D6 and D8 days appear to decline significantly(P<0.01). The expression of Ang-Ⅱ mRNA and protein significantly increased in the high insulin model(P<0.05). Conclusions High insulin can increase the expression of Ang-Ⅱ in the endometrium of pregnant rats during the process of pregnancy. It is suggested that insulin may have an effect on the angiogenesis in the endometrium of the uterus.

Effect of simvastatin on angiotensin II-stimulated secretion and proliferation of adrenocortical carcinoma H295R cells

2017, 37(3):  346-350. 

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Objective To investigatethe potential effects of simvastatin on angiotensin II-stimulatedsecretion and proliferation of adrenocortical carcinoma H295R cells. Methods The H295R cells were divided into control group, Angiotensin II group, simvastatin group and Angiotensin II plus simvastatin group. Cortisol in medium was determined by chemiluminescent method, and aldosterone was determined by radioimmunoassay. The mRNA expressions of 11 beta-hydroxylase(CYP11B1) and aldosterone synthase(CYP11B2) were examined by real-time quantitative PCR. Cell proliferation was detected by MTS method. Results Compared with control group, angiotensin II increased the secretion of cortisol and aldosterone, and the expression of CYP11B1 and CYP11B2. Simvastatin decreased cortisol secretion and CYP11B1 mRNA expression (P < 0.05). Simvastatin also inhibited angiotensin II-induced the secretion of cortisol and aldosterone, and the expression of CYP11B1 and CYP11B2 compared with Angiotensin II group(P < 0.05). Angiotensin II had no effects on the cell proliferation, while simvastatin significantlyinhibited cell proliferation. The inhibitory effect of simvastatin on proliferation was enhanced when simvastatin combined with angiotensin II(P < 0.05). Conclusions Simvastatin can inhibit angiotensin II-induced secretion of cortisol and aldosterone in H295R cells. Simvastatin inhibits cell proliferation, which could be enhanced by simvastatin combined with angiotensin II.

Overexpression of MST1 inhibits the proliferation, migration and invasion of cervical cancer cell line SiHa

2017, 37(3):  351-354. 

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Objective To discuss the influence of MST1 (mammalian sterile 20-like kinase 1，MST1) on the proliferation，migration and invasion of SiHa cervical cancer. Methods Western blot was used to detect the expression of MST1 in cervical epithelial cells H8 and cervical cancer cells SiHa; PJ3H-HA-MST1 was constructed and transfected it to SiHa cell by LipofectamineＴＭ3000; MST1、 HA、Ki-67 and MMP9 protein expression were evaluated by Western blot; While the proliferation，migration and invasion of SiHa cell were assessed by MTS、scratch adhesion test and Transwell assay respectively. Results Compared SiHa cells with H8 cells，MST1 expression in SiHa cells was significantly lower than that of H8 cells．The plasmid was successfully transfected into SiHa cells, MST1 expression was significantly higher, while the expression of Ki-67 and MMP9 were sharply lower, and the proliferation、migration and invasion ability were all suppressed significantly. Conclusions Overexpression of MST1 can inhibit the proliferation、migration and invasion of cervical cancer cell line SiHa.

Association between the polymorphisms of HIF-1α gene -1496 and hypoxic tolerance in cyanotic congenital heart disease

2017, 37(3):  355-359. 

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Objective To identify the gene polymorphism of HIF-1α associated with hypoxic tolerance in CCHD. Methods HIF-1α was selected sequenced in 97 CCHD cases and 108 ACHD cases. Different distribution of polymorphic site was detected after bioinformatics analysis. The related variants were confirmed using Sanger Sequencing. For the polymorphic site in promoter region of HIF-1α, different genotype human HIF-1α promoter-luciferase reporter gene vectors were constructed respectively, and the vectors were transiently transfected into HEK293T and H9C2 cells. Dual-Luciferase assay was performed to reflect the transcriptional activity of different genotype HIF-1α promoter. Results The mutation rate of HIF-1α -1496 in CCHD population was significantly higher than that in ACHD population (P<0.05). Transcriptional activity of DD genotype was higher than that of AA genotype (P<0.05). Conclusions HIF-1α -1946 is associated with hypoxia tolerance of CCHD. Transcription activity of DD genotype was significantly increased, which may be one of the genetic mechanisms of hypoxia tolerance.

UVB induces morphological changes and the expression of MMPs in human fibroblasts

2017, 37(3):  360-363. 

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Objective To observe the changes of telomere length and MMPs level in human fibroblasts induced by UVB, and to explore their roles onskin photoaging. MethodsHuman skin fibroblasts was extracted and culture.The 5th fibroblasts were irradiated by UVB. The morphology of fibroblasts were observed, and the length of telomere and the mRNA expression of COL1a1 and hTERT were detected by QRT-PCR. The expression of MMP-3 and MMP-1 were detected by Western blot. Results The fibroblastsgradually became round, wrinkled and disordered after 30mJ/cm2 UVB irradiation for 24h. The mRNA level of COL1a1 and hTERT and the expression of MMP-3 and MMP-1 weresignificantly increased after UVB irradiation compared with control, and the length of telomere was shortened. Conclusions UVB might initiate the early process of photoaging by the morphological changes of human skin fibroblasts and increasing the expression of MMP-3 and MMP-1.

Down-regulation of PTEN expression promotes the adhesion in activated rat hepatic stellate cells in vitro

2017, 37(3):  364-368. 

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Objective To investigate the down regulation of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) gene by adenovirus mediated short hairpin RNA ( shRNA) on the adhesion in activated hepatic stellate cells（HSC）in vitro and the related signal transduction mechanism. Methods The recombinant adenovirus (Ad-shRNA/PTEN) with shRNA targeting PTEN and expressing green fluorescent protein (GFP) were transient transfected into the cultural activated HSC in vitro. The experimental group as follows: 1) Control group, viral medium was replaced by DMEM at virus transfection step. 2) Ad-GFP group, HSC were infected with adenovirus expressing GFP alone. 3) Ad-shRNA/PTEN group, HSC were infected with adenovirus both taking shRNA targeting PTEN and expressing GFP. PTEN mRNA expression was detected by real-time fluorescent quantitation PCR, and western blot was used for detecting protein expressions of PTEN, focal adhesion kinase (FAK) and phosphorylated FAK (Thr397) ［p-FAK（Tyr397）］in HSC. The toluidine blue stain method and MTT colorimetric method were used to determine the adhesion ability of HSC. Results When HSC were infected by adenovirus for 48 hours, PTEN protein and mRNA expressions in Ad-shRNA/PTEN group significantly decreased (P＜0.05), compared to control group and Ad-GFP group, and the expressions of p-FAK (Tyr397) in Ad-shRNA/PTEN group were significantly higher than those in control group and Ad-GFP group (P＜0.05). The adhesion cell number and the adhesion rate of HSC in Ad- shRNA/PTEN group significantly increased, compared with control group and Ad-GFP group (P < 0.05). Conclusions The down-regulation of PTEN expression can promote the adhesion by increasing the activation of FAK signaling transduction in activated HSC in vitro.

Construction of BCR-ABL SH3-T79Y mutant recombinant adenovirus vectors and its promotion on apoptosis of K562/G01 cells

2017, 37(3):  369-375. 

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Objective: To construct BCR-ABL SH3-T79Y mutant recombinant adenovirus vectors and investigate its effects on apoptosis of K562/G01 cells. Methods: SH3-T79Y mutant was amplified by overlapping PCR with pMig210 as template and cloned into recombinant adenovirus vectors. After identifying, packaging and amplifying, the recombinant adenovirus vectors containing SH3-T79Y mutant was obtained. Recombinant adenovirus vectors were transferred into K562/G01 cells. Then transfection efficiency was determinated, changes of cell morphology were observed by Wright's staining, cell apoptosis was evaluated by flow cytometry, BCR-ABL and CrkL phosphorylation was detected by Western-blot. Results: The vectors were successfully constructed. Transfection efficiency was more than 80% after transferring into K562/G01 cells for 72h; there was obvious apoptosis phenomenon, cell apoptosis significantly increased to 32.46% compared with the control groups (P＜0.05), BCR-ABL and CrkL phosphorylation significantly decreased and so did the expression of BCR-ABL(P＜0.05). Conclusion: Successfully constructed the SH3-T79Y mutant recombinant adenovirus vectors and proved it could promote the apoptosis of K562/G01 cells by inhibiting BCR-ABL and CrkL phosphorylation.

Expression of PTPN13 in human gastric cancer and gastric cancer SGC-7901 cell line and its association with proliferation and invasion

2017, 37(3):  376-381. 

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Objective: to investigate the expression of PTPN13 in human gastric cancer and gastric cancer SGC-7901 cell line and its association with proliferation and invasion. Methods: 106 cases gastric cancer tissue samples and matched normal peritumorial tissues were collected. SGC-7901 cells were cultured and divided into two groups including pcDNA3.1-PTPN13 transfection group and without transfection group. Immunohistochemical technique were used to detected protein expression of PTPN13. The association of PTPN13 expression with tumor location, tumor size, depth of invasion and tumor metastasis was analyzed. The survival rate of patients with different PTPN13 expression was calculated by Kaplan-Meier curves. CCK-8 assay was used to estimate the proliferation changes. Using Trans-well assay analyzed the invasion of SGC-7901 cells. Moreover, Western blot was performed to detect the markers of EMT including E-cadherin, Snail and MMP9.Results: Immunohistochemistry showed the expression rate of PTPN13 in gastric cancer tissues was lower than normal tissue (31 % vs.83%, P<0.05). The expression of PTPN13 in patients was related to with different tumor size, depth of invasion and tumor metastasis (P<0.05). The 2-years survival rate of patients with negative PTPN13 expression were lower. Overexpression reduced both proliferation and invasion in SGC-7901 cells. Western blot showed that up-regulated PTPN13 could increase E-cadherin level but decreased the level of Snail and MMP9.Conclusions: PTPN13 play a role in gastric cancer tissue and cells as a tumor suppressor. Lower PTPN13 may indicate a poor prognosis. PTPN13 could be used as therapy target in gastric cancer.

Analysis and strategies of doctor-patient nervous relationship of psychological factors at current society

2017, 37(3):  382-385. 

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Improving the doctor-patient relationship is a long-term and complex process.that can't be available and unilateral in a short time. But we can’t do nothing because of the difficult. The psychological cause of the doctor-patient nervous relationship is a pointcut that starts from me and now on.

Bisoprolol increases myocardial SERCA2a activity in rats with heart failure

2017, 37(3):  386-390. 

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Objective To investigate the effects of bisoprolol on myocardial SERCA2a activity in rats with heart failure．Methods Male SD rats were randomly divided into normal control group(control group), sham operation group(sham group), model group, bisoprolol group（Bis group）, captopril group(Cap group) and bisoprolol plus captopril group[(Bis＋Cap）group], heart failure rat model was induced by intraperitoneal injections of doxorubicin. Distilled water, bisoprolol, captopril or bisoprolol plus captopril were administrated by gastrogavage for 35 days， respectively. Indices of cardiac function and plasma levels of B-type natriuretic peptide (BNP) were measured, myocardial expression of miR-25-3p was detected by Stem-loop RT-qPCR, myocardial levels of SERCA2a and phospholamban(PLB) were detected by western blot analysis, myocardial SERCA2a activity was determined by the inorganic phosphorus method. Results Cardiac function in model group decreased significantly while plasma levels of BNP were significantly higher than those of control group(P＜0.01). Myocardial expression of miR-25-3p in model group was significantly higher while myocardial levels of SERCA2a and PLB，SERCA2a activity were significantly lower than those of control group(P＜0.01). Cardiac function in Bis group, Cap group and Bis＋Cap group improved significantly while plasma levels of BNP were significantly lower than those of model group(P＜0.01). Myocardial expression of miR-25-3p in Bis group, Cap group and Bis＋Cap group were significantly lower while myocardial levels of SERCA2a and PLB were significantly higher than those of model group(P＜0.01). The SERCA2a/PLB ratio and SERCA2a activity in Bis group and Bis＋Cap group were significantly higher than those of model group(P＜0.05). Conclusions Bisoprolol therapy improves cardiac function in rats with heart failure, which may be related to inhibition of myocardial miR-25-3p, increasing myocardial SERCA2a and PLB levels, enhancing SERCA2a activity.

Nicotine promotes the apoptosis of human umbilical vein endothelial cells

2017, 37(3):  391-395. 

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Objective To research different concentrations of nicotine promotes the apoptosis of human umbilical vein endothelial cells and its mechanism. Methods Human umbilical vein endothelial cells were cultured in vitro. According to the concentration of nicotine, grouped as 10-6, 10 -7, 10-8 mol/L, and control.After 24 h of nicotine treatment, using CCK-8 assay tests the proliferation/activity; Annexin V - FITC fluorescence staining detects the apoptotic cells. Western blot detects the expression of protein Bax, Bcl - 2 and PARP-1, to study the mechanism of apoptosis.Results Compared with control group, in 10-6,10-7mol/L groups the proliferation/activity of HUVECs decreased(P<0.05). And the number of apoptotic cells increased(P<0.01).The target proteins expression of 10-6、10-7mol/L groups were changed as follow: Bax increased(P<0.01)，Bcl-2 decreased (P<0.01) and PARP-1 increased(P<0.01). Conclusion Concentrations of nicotine could promote the apoptosis of vascular endothelial cells, which accelerates the development of atherosclerotic disease.

Dynamic monitoring platelet function in a traumatic brain injury patient after percutaneous coronary intervention

2017, 37(3):  396-398. 

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Traumatic brain injury is tricky to deal wih due to various factors like severe condition, complicated diagnosis and treatment,rapid development of illness,multiple complications at the later stage along with high fatality rate. In this case, the patient had taken dual anti-platelet drugs in long term after operation.It’s of tremendous importance to closely monitor the platelet function of the patient so as to balance between the prevention of bleeding and possible heart diseases.The assay reports one case of applying dynamic platelet function monitoring after operation.

A novel method to predict potential target genes of piRNAs using “ping-pong” theory

2017, 37(3):  399-401. 

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miR-148a inhibits the migration and invasion of lung adenocarcinoma cell

2017, 37(3):  402-404. 

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Research progress on mechanism of autophagy in intestinal mucosal barrier function

2017, 37(3):  405-409. 

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Autophagy is a biological process which cells maintain homeostasis through degradation of cytoplasmic macromolecules and damaged organelles by membrane vesicle structure. Autophagy plays a critical role in maintaining survival of intestinal epithelial cells during intestinal mucosal barrier dysfunction. A negative regulator of autophagy can lead to intestinal inflammation and tumorigenesis.

Role and molecular mechanism of miR-320 in the genesis and progression of tumors

Hui TANG

2017, 37(3):  410-414. 

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miR-320 is a newly discovered microRNA. In recent years, the abnormal expression of miR-320 has been found in various kinds of tumors. Therefore, its role and molecular mechanism in the tumorigenesis and tumor progression has getting more and more attention.

Controversy of soluble urokinase-type plasminogen activator receptor in gastrointestinal cancer

2017, 37(3):  415-418. 

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Soluble urokinase- type plasminogen activator receptor (suPAR) is the soluble form of urokinase- type plasminogen activator receptor (uPAR). Besides being a new biomarker of inflammation, suPAR plays an important role in the growth, development, invasion and metastasis of gastrointestinal cancer. Recent research suggests that suPAR has significant value in the molecular diagnosis of incipient tumors, monitoring of the therapeutic response and further development of the molecular targeted new agents.

Progress in the study of epigenetic regulation of airway smooth muscle cells in asthma

2017, 37(3):  419-421. 

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Hyperplasia of smooth muscle cells is a major aspect of airway remodeling in asthma.In recent years, people gradually found that the epigeneticsplays an important role on the proliferation of airway smooth muscle cells and the secretion of inflammatory cytokines, including DNA methyltransferase inhibitors to inhibit the phenotype transformation; histone acetylation related with hypertrophy. In addition, the Mir-RNA can be related with the regulation of a variety of physiological functions of airway smooth muscle cells of asthma model, including inhibition of proliferation and release of inflammatory factors. Hope that epigenetics can become a new target for the treatment of asthma.

Research progress in mechanisms of opioids-induced respiratory depression

2017, 37(3):  422-426. 

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Respiratory depression is a common adverse reaction of opioids.Currently, the mechanisms and treatment of opioid-induced respiratory depression (OIRD) are one of the research highlights. OIRD arises from stimulation of μ-opioid receptors in the pre-Botzinger complex and the Kolliker-Fuse neurons. Adenylyl cyclase, calcium channels, and G-protein–gated inwardlyrectifying potassium (GIRK) channels may be the key cellular signaling mechanisms of OIRD.

"Internet + precision medicine " promote informationlization and integration of medical courses

2017, 37(3):  427-430. 

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In the context of medical higher education and information depth integration, we carry out the "pathology" blended-teaching based on the Blackboard. The " precision medicine " philosophy as a guide, in accordance with the different characteristics of students individual teaching design. At the same time, we cultivate students comprehensive thinking mode and guide students through the analysis of the clinical data to integrate medical disciplines related knowledge through blended learning. And establish evaluation scheme of students, a comprehensive analysis of the use of big data of student assessment results. In order to promote the student individual as the center of the "precision teaching" information technology development process.

Exploration of online learning mode for refresher doctors prehospital training and evaluation of learning effect in Peking Union Medical College Hospital

2017, 37(3):  431-434. 

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Objective Carry out the reform of the online learning mode of prehospital training for refresher doctors, and evaluatethe effect of reform in Peking Union Medical College Hospital. Methods An exploration was carried out on the refresher doctors prehospital training mode from traditional classroom teaching to online learning, Established the online learning system. Evaluated the effect of online learning by the way of questionnaire investigation and reexamination among 289 refresher doctors.Results 289 refresher doctors completed the structured online courses of prehospital training, and the average pass rate of the first test was 42.2%. The questionnaire and retest was conducted ,A total of 262 doctors completed the work, The recovery rate was 90.7%,and the average pass rate of retest was 74.9%, improved 32.7%. Conclusions Online learning mode is an effective tool for knowledge dissemination,and has a better learning effect.