Abstract: Objective To explore effects of human embryonic stem cells ( hESCs ) onhproliferation , invasion and migration abilities of SK-Hep1 humanhepatoma cells in the co-culture micro environmen of hESCs and SK - Hep1. Methods Single cultured SK-Hep1 cells were served as control group while SK-Hep1 which non-contact co-cultured with hESCs was regarded as experimental group.The hproliferation ability of SK-Hep1 was measured by MTT method; invasion and migration ability of SK-Hep1 cells were detected by Transwell chamber method ; the nucleus variation and cell apoptosis of SK-Hep1 were detected by Hoechst33258 chromosome and flow cytometry. ResultsThe proliferation ability of SK-Hep1 cells in the experimental group was inhibited obviously compared with control group (P <0.05); the number of SK - Hep1 cells which passed through the Transwell chambers were reduced significantly compared with control group in invasion and migration experiment(P<0.05); more nucleus pycnosis and deformation appeared in experimental group than that in control group .And apoptosis rate of SK- Hep1 cells in the experimental group was significantly higher than that of in the control group (P <0.05). Conclusions Human embryonic stem cells have inhibitory effect on SK-Hep1 human hepatoma cell line.

Key words: embryonic stem cells, hepatoma cell, inhibition