Abstract: Objective To investigate the effect of high level insulin on the vascular marker molecules Ang-Ⅱ in the endometrium of early pregnant mice. Methods The high insulin animal model was constructed by subcutaneous injection of insulin. The uterine tissues and serum of D6, D7, and D8 days of pregnancy which were collected after mating with normal male rats, and blood glucose was monitored simultaneously. In addition to the male rats with vasectomized mating to construct artificial decidualization. ELISA for the detection of serum insulin, progesterone, and estradiol changes, real time PCR to detect the Ang-Ⅱ mRNA expression in endometrial tissue. Western blot for the detection of Ang-Ⅱ protein expression in uterine tissue. Results Insulin in the high insulin group of pregnancy D6, D7, and D8 days increased significantly(P<0.001), estrogen levels in pregnancy D8 days appear significantly decreased(P<0.05) and progesterone levels in pregnancy D6 and D8 days appear to decline significantly(P<0.01). The expression of Ang-Ⅱ mRNA and protein significantly increased in the high insulin model(P<0.05). Conclusions High insulin can increase the expression of Ang-Ⅱ in the endometrium of pregnant rats during the process of pregnancy. It is suggested that insulin may have an effect on the angiogenesis in the endometrium of the uterus.

Key words: Key words: Insulin, Decidualization, ANG-Ⅱ, Angiogenesis