Abstract: Objective To investigate the effect and mechanism of silencing ATP1A1 gene on invasion ability of human U251 glioma cells. Methods The human U251 glioma cells were infected with lentivirus expressing shRNA-ATP1A1. The mRNA and protein expressions of ATP1A1 in U251 glioma cells were detected by RT-qPCR and Western blot，respectively. The proliferation of U251 glioma cells was determined by MTT assay.The migration and invasion ability were detected by cell scratch test and Transwell chamber. The protein expression of matrix metallo proteinase 2 (MMP-2) and MMP-9 in U251 glioma cells were detected by Western blot. Results The mRNA and protein expression of ATP1A1 in the silence group were significantly inhibited,The ability of proliferation, migration and invasion were also significantly inhibited (P<0.05),The expression of MMP-2 and MMP-9 were also significantly reduced,there was a significant difference(P<0.05). Conclusions RNA interference targeting ATP1A1 gene can significantly inhibit the proliferation, migration and invasion of glioma U251 cells.The mechanism might be related to the down-reguLation of MMP-9 and MMP-2.ATP1A1 can be used as a potential target for the treatment of glioma.

Key words: Key words: ATP1A1, RNA interference, glioma, invasion, mechanism